We narrowed to 12,157 results for: pcDNA

Showing: 2681 - 2720 of 12157 results

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pcDNA5FRT/TO-UBE2QL1-Strep/HA

Plasmid

#124666

Purpose

Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4-GFP-Metavinculin

Plasmid

#107151

Purpose

mammalian expression

Depositor

Tina Izard

Article

Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

Insert

metavinculin (VCL Human)

Tags

(His)10-GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K5-9R

Plasmid

#63562

Purpose

Expression of human H2A with K5R and K9R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 5 and Lysine 9 are now Arginine

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-ORF10-2xCSTREP

Plasmid

#136174

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF10

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF10

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ShhN-Ren

Plasmid

#37677

Depositor

Philip Beachy

Article

Ma et al Cell. 2002 Oct 4;111(1):63-75.

Insert

shh (Shh Mouse)

Tags

renilla luciferase

Expression

Mammalian

Mutation

soluble form (see comments)

Promoter

CMV

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-Hbxadr-Hatag

Plasmid

#24930

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Tags

HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CTLA4-mgSrtA

Plasmid

#125794

Purpose

for co-expression of CTLA4 and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD80/86 positive cells.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CTLA4-mgSrtA

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_DW164AA USP7

Plasmid

#131252

Purpose

Mammalian expression of N-terminally Myc-tagged DW164AA USP7

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Contains a point mutation that converts USP7 aspa…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp9

Plasmid

#157703

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp9 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA HA FAK Y925F

Plasmid

#50509

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y925F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)- e18 LOF

Plasmid

#131729

Purpose

Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-Kif18b-24xGCN4_v1

Plasmid

#72228

Purpose

A fusion of the Kif18b motor protein to the v1 SunTag peptide array

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Kif18b (KIF18B Human)

Expression

Mammalian

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 1-400

Plasmid

#45754

Depositor

Robert Lefkowitz

Article

Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

Insert

MDM2 1-400 (MDM2 Human)

Expression

Mammalian

Mutation

aa 1-400, lacks catalytic RING domain

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-Cezanne-Strep-HA

Plasmid

#113492

Purpose

Expression of human Cezanne with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

Cezanne (OTUD7B Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag-Eya2

Plasmid

#49264

Purpose

expresses Flag tagged Eya2

Depositor

Heide Ford

Insert

Eya2 (EYA2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-mito-ZifCY1

Plasmid

#36317

Depositor

Amy Palmer

Article

Dittmer et al J Biol Chem. 2009 Jun 12;284(24):16289-9

Insert

mito-ZifCY1 genetically encoded Zn(II) sensor

Tags

see comment

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.T7 delta-NFAT3

Plasmid

#28224

Depositor

Sebastien Jauliac

Article

Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

Insert

Delta NFAT3 (NFATC4 Human)

Tags

T7

Expression

Mammalian

Mutation

hNFAT3 WT deleted amino acids 1-342

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPH2

Plasmid

#19454

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 4 (HSPA4 Human)

Expression

Mammalian

Mutation

mutations A381T, T732A, and an insertion of "…

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp10

Plasmid

#157706

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp10 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 MDM2 S186D

Plasmid

#16235

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Ser186 replaced by Asp

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-AktAR-Kras

Plasmid

#123357

Purpose

FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

Plasmid

#129513

Purpose

Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 C88S (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-PKHD1-myc

Plasmid

#108407

Purpose

Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

Depositor

Gregory Germino

Article

Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

Insert

PKHD1 (PKHD1 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H2A-TORCAR

Plasmid

#175016

Purpose

TORCAR targeted to the nucleus via histone H2A.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

H2A-TORCAR

Tags

Histone H2A

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Setd3-Flag-pcDNA3.0

Plasmid

#49144

Purpose

mammalian expression of Flag tagged Setd3

Depositor

Jing H Wang

Article

Chen et al Haematologica. 2013 May;98(5):739-43. do

Insert

Setd3 (Setd3 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-mTagBFP2-MRTF-B

Plasmid

#182062

Purpose

Mammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mTagBFP2-MRTF-B (Mrtfb Mouse)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag7_SKL

Plasmid

#175532

Purpose

Peroxysomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-SKL

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS4A-2K-NS4B

Plasmid

#120828

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS4A-2K-NS4B ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pZ143-pcDNA3-BvCas12b

Plasmid

#122445

Purpose

Expresses BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BvCas12b-NLS-3xHA

Tags

3XHA

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-WT-VLCAD-FLAG

Plasmid

#55823

Purpose

Expresses WT-VLCAD in mammalian cells

Depositor

Yoshimi Homma

Article

Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

Insert

Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA HisB TO rFez1

Plasmid

#17385

Depositor

Stephen Goff

Article

Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

Insert

FEZ1 (Fez1 Rat)

Tags

his and myc

Expression

Mammalian

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-Dronpa-Kras

Plasmid

#182329

Purpose

Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Dronpa-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-Venus

Plasmid

#87613

Purpose

Actin-targeted Venus (YFP).

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-Venus

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nprot

Plasmid

#157729

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nprot (N SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp9-HF

Plasmid

#157705

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp9-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3 Flag MKK7a1

Plasmid

#14538

Depositor

Roger Davis

Article

Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

MKK7a1 (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-FH-Nsp9

Plasmid

#157704

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp9 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-FRT-TO-FH-Nsp10

Plasmid

#157707

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp10 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1-CnA-H151A

Plasmid

#191956

Purpose

Expression of phosphatase dead mutant of calcineurin A (CnA-H151A).

Depositor

Luca Scorrano

Insert

Calcineurin subunit alpha H151A

Expression

Mammalian

Mutation

H151A

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-sfGFP-24xGCN4_v1

Plasmid

#61056

Purpose

A direct fusion of sfGFP to the 24xGCN4_v1 peptide array

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

sfGFP-24xGCN4_v1 peptide array

Expression

Mammalian

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

Showing: 2681 - 2720 of 12157 results

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We narrowed to 12,157 results for: pcDNA