We narrowed to 76,945 results for: rest

1. pX459-HypaCas9-mR2-TUBA1B_sgRNA

   Plasmid

   #183888

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 20XUAS IVS Jaws mCherry_tr

   Plasmid

   #111549

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

   Depositor

   Vivek Jayaraman

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws_mCherry_trafficked
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. HOXB7 (human) pCMV

   Plasmid

   #8539

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB7 (HOXB7 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

4. HOXB4 (human) FLAG MSCV

   Plasmid

   #8528

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB4 (HOXB4 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Missing N-terminal 14 amino acids; improved Ribos…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

   Plasmid

   #129531

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIC3Nme
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. 10XUAS IVS Jaws mVenus_tr

   Plasmid

   #111553

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

   Depositor

   Vivek Jayaraman

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws_mVenus_tr
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS2044

   Plasmid

   #79652

   Purpose

   ssAAV genome with Ple255 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

   Insert

   ssAAV Ple255-EmGFP WPRE
   

   Use

   AAV

   Promoter

   PAX6 HS234Z Enhancer

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. 13XLexAop2 IVS Jaws mCherry_tr

   Plasmid

   #111551

   Purpose

   Optogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherry

   Depositor

   Vivek Jayaraman

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws_mCherry_trafficked
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FusXTBE-1397-C

   Plasmid

   #201398

   Purpose

   Expresses C terminus half of DddAtox in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

   Insert

   COX8A MTS-LacZ-G1397 DddAtox-C-UGI-ATP5B 3'UTR
   

   Expression

   Mammalian

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. FusXTBE-1397-N

    Plasmid

    #201397

    Purpose

    Expresses N terminus half of DddAtox in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    COX8A MTS-LacZ-G1397 DddAtox-N-UGI-ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HOXB4 (human) HA-TAT-tag pET

    Plasmid

    #8526

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Tags

    HA

    Expression

    Bacterial

    Mutation

    replace T7 and HIS tags of pET with an HA tag;

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. p221- purVSG117UTR

    Plasmid

    #59732

    Purpose

    Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

    Depositor

    Gloria Rudenko

    Article

    Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

    Insert

    VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
    

    Use

    For homologous recombination into vsg221 expressi…

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-sgCCR5

    Plasmid

    #83930

    Purpose

    Lentiviral vector expressing an sgRNA targeting CCR5 NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCCR5
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFLAG-4S Tetherin

    Plasmid

    #41073

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S74, S84, 137S, and 144S

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-HDAC4 C-terminus

    Plasmid

    #71400

    Purpose

    CAG promoter, mammalian expression

    Depositor

    Bo Chen

    Article

    Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

    Insert

    HDAC4 C-terminus (HDAC4 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pEMS2049

    Plasmid

    #79657

    Purpose

    ssAAV genome with Ple260 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    ssAAV Ple260-EmGFP WPRE
    

    Use

    AAV

    Promoter

    PAX6 Retinal Enhancer

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFLAG-137.144S Tetherin

    Plasmid

    #41072

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    137S, 144S

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pEMS2045

    Plasmid

    #79653

    Purpose

    ssAAV genome with Ple256 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    ssAAV Ple256-EmGFP WPRE
    

    Use

    AAV

    Promoter

    PAX6 enhancer

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS2048

    Plasmid

    #79656

    Purpose

    ssAAV genome with Ple259 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    ssAAV Ple259-EmGFP WPRE
    

    Use

    AAV

    Promoter

    PAX6 MiniPromoter

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-HDAC4 1-747

    Plasmid

    #71399

    Purpose

    CAG promoter, mammalian expression

    Depositor

    Bo Chen

    Article

    Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

    Insert

    HDAC4 1-747 (HDAC4 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only