We narrowed to 22,079 results for: his

1. pEXP5-NT/6xHis mCherry F30 Broccoli

   Plasmid

   #169234

   Purpose

   Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with Broccoli RNA aptamer.

   Depositor

   Dora Tang

   Article

   Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

   Insert

   6xHis mCherry F30-Broccoli
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCI-T7Max-UTR1-NTerminus8xHis-T500

   Plasmid

   #178421

   Purpose

   Empty backbone. T7Max promoter with N-His Tag

   Depositor

   Kate Adamala

   Article

   Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7Max

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSumo-His6-SUMO-AtTPR2(1-209)

   Plasmid

   #177860

   Purpose

   Bacterial Expression of TPR2

   Depositor

   Karsten Melcher

   Article

   Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

   Insert

   TPR2 (TPR2 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSumo-His6-SUMO-OsTPR2(1-210)

   Plasmid

   #177859

   Purpose

   Bacterial Expression of TPR2

   Depositor

   Karsten Melcher

   Article

   Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

   Insert

   TPR2
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSUMO His6 SUMO SnRK2.3 (19-361)

   Plasmid

   #177846

   Purpose

   Bacterial Expression of SnRK2.3

   Depositor

   Karsten Melcher

   Article

   Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

   Insert

   SNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET Duet1 His6 SUMO D14

   Plasmid

   #177854

   Purpose

   Bacterial Expression of D14

   Depositor

   Karsten Melcher

   Article

   Zhao et al Cell Res. 2013 Mar;23(3):436-9. doi: 10.

   Insert

   D14
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET24a His6 GST PYL2(14-188)

   Plasmid

   #177842

   Purpose

   Bacterial Expression of PYL2

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   PYR1-like 2 (PYL2 Mustard Weed)
   

   Use

   Tags

   6xHis-GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pnEA-vH_His-TEV-DSep1-msfGFP

   Plasmid

   #174494

   Purpose

   bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Insert

   Sep1 (Sep1 Fly)
   

   Use

   Tags

   His6-TEV and msfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-Fgβ-anticalin-His-ybbr

   Plasmid

   #168040

   Purpose

   Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag, Fgβ peptide, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b p97-Strep-His E314Amb

    Plasmid

    #169021

    Purpose

    Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Use

    Tags

    His and SBP

    Expression

    Bacterial

    Mutation

    E314TAG

    Promoter

    T7

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-EGFP-DmTral_394-543-V5His6_D

    Plasmid

    #146134

    Purpose

    Insect Expression of DmTral_394-543

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral_394-543 (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent mutations (nucleotides A198C, A387G) a…

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169162

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    6His-3xFlag

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

    Plasmid

    #169163

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E

    Plasmid

    #146166

    Purpose

    Insect Expression of DmUPF2-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmUPF2-siRNAres (Upf2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    3 non silent mutations: L1022P, V1097L and N1213S…

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C

    Plasmid

    #146013

    Purpose

    Insect Expression of DmSmaug_763-999

    Depositor

    Elisa Izaurralde

    Insert

    DmSmaug_763-999 (smg Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    2 silent mutations and a deletion of the AA 961-9…

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

    Plasmid

    #146001

    Purpose

    Insect Expression of DmSmaug_1-762

    Depositor

    Elisa Izaurralde

    Insert

    DmSmaug_1-762 (smg Fly)
    

    Use

    Tags

    V5-6xHis

    Expression

    Insect

    Mutation

    NP_523987.1, 7 silent mutations compared to the s…

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET51b-His-TEV-SNAP-tag_dead_mt

    Plasmid

    #167270

    Purpose

    Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    C145A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET51b-His-TEV-SNAP-tag_fast_dead_mt

    Plasmid

    #167272

    Purpose

    Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R-C145A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQC hKGA.S286A V5-His IRES G418

    Plasmid

    #110391

    Purpose

    γ-Retroviral transfer vector for expressing glutminase isoform KGA (S286A), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    KGA glutaminase (GLS Human)
    

    Use

    Retroviral

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    S286A

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDB.His.MBP.3C CARD8 CARD A452C R459E

    Plasmid

    #164024

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    A452C, R459E

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDB.His.MBP.3C CARD8 CARD A452C R464E

    Plasmid

    #164025

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    A452C, R464E

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDB.His.MBP.3C CARD8 CARD A452C E485R

    Plasmid

    #164026

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    A452C, E485R

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDB.His.MBP.3C CARD8 CARD A452C E490R

    Plasmid

    #164027

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    A452C, E490R

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDB.His.MBP.3C CARD8 CARD A452C R495E

    Plasmid

    #164028

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    A452C, R495E

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDB.His.MBP.3C CARD8 CARD A452C D511K

    Plasmid

    #164029

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    A452C, D511K

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDB.His.MBP.3C CARD8 CARD A452C Y527A

    Plasmid

    #164030

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    A452C, Y527A

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDB.His.MBP.3C NLRP1 UPA-CARD E1461R

    Plasmid

    #164007

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    E1461R

    Promoter

    Available since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDB.His.MBP.3C NLRP1 UPA-CARD W1460A

    Plasmid

    #164006

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    W1460A

    Promoter

    Available since

    Feb. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDB.His.MBP.3C NLRP1 UPA-CARD M1457A

    Plasmid

    #164005

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    M1457A

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDB.His.MBP.3C NLRP1 UPA-CARD Y1445A

    Plasmid

    #164004

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Y1445A

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pQE80L-His-SpyTag-XMod-Doc(WT)

    Plasmid

    #157675

    Purpose

    Expression of XMod-Doc wild type with His6x tag and SpyTag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    XMod-Doc Wild type with Histag and SpyTag
    

    Use

    Tags

    Histag and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPICZ-His6-K2P4.1A-mCherry-StreptagII

    Plasmid

    #158743

    Purpose

    Expresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.

    Depositor

    Arthur Laganowsky

    Article

    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

    Insert

    K2P4.1-A (KCNK4 Human)
    

    Use

    Tags

    His6 and mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Residues 1-264 with N-linked glycosylation sites …

    Promoter

    AOX1

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPICZ-His6-K2P4.1B-mCherry-StreptagII

    Plasmid

    #158744

    Purpose

    Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

    Depositor

    Arthur Laganowsky

    Article

    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

    Insert

    K2P4.1-B (KCNK4 Human)
    

    Use

    Tags

    His6 and mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Residues 1-290 and N-linked glycosylation sites r…

    Promoter

    AOX1

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET15-His-∆NGlpG(C104A/M247C)

    Plasmid

    #155133

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C104A/M247C, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-Avi-Coh (E154C)-HIS

    Plasmid

    #153447

    Purpose

    E. coli expression of Rc. CohE construct with E154C mutation.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.CohE
    

    Use

    Tags

    6xHis and Avi-tag

    Expression

    Bacterial

    Mutation

    E154C mutation on CohE

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1/NDUFS2 _34-66/myc-His

    Plasmid

    #127495

    Purpose

    Expresses myc-tagged _34-66 NDUFS2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 _34-66 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 _34-66

    Promoter

    CMV

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1/NDUFS2 E57A/myc-His

    Plasmid

    #127497

    Purpose

    Expresses myc-tagged NDUFS2/E57A in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 E57A (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 E57A

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1/NDUFS2 T58A/myc-His

    Plasmid

    #127498

    Purpose

    Expresses myc-tagged NDUFS2/T58A in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 T58A (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 T58A

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1/NDUFS2 H60A/myc-His

    Plasmid

    #127499

    Purpose

    Expresses myc-tagged NDUFS2/H60A in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 H60A (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 H60A

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1/NDUFS2 W61A/myc-His

    Plasmid

    #127500

    Purpose

    Expresses myc-tagged NDUFS2/W61A in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 W61A (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 W61A

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1/NDUFS2 K62A/myc-His

    Plasmid

    #127501

    Purpose

    Expresses myc-tagged NDUFS2/K62A in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 K62A (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 K62A

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1/NDUFS2 P63A/myc-His

    Plasmid

    #127502

    Purpose

    Expresses myc-tagged NDUFS2/P63A in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 P63A (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 P63A

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. KG703: pMVP (L5-L4) 6x His epitope tag

    Plasmid

    #121706

    Purpose

    pMVP L5-L4 entry plasmid, contains 6x His epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    6x His epitope tag (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. ELK1 A box-NLS pcDNA3.1/HisC

    Plasmid

    #121096

    Purpose

    Expression of the A box fragment of ELK1

    Depositor

    Paul Herring

    Article

    Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Tags

    6XHis-Omni-xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. ELK1 B box-NLS pcDNA3.1/HisC

    Plasmid

    #121095

    Purpose

    Expression of the B box fragment of ELK1

    Depositor

    Paul Herring

    Article

    Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Tags

    6XHis-Omni-xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347)

    Plasmid

    #115429

    Purpose

    Selection for in-frame fusion expression constructs

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Type

    Empty backbone

    Use

    Tags

    T2A-SpHIS5 and λN

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

    Plasmid

    #115432

    Purpose

    Vector for expressing RPL28 with mutagenized exon2

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    exon 2 deleted, with MfeI / NheI cloning site

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. TRSP-COMP-blac-flag-his

    Plasmid

    #110964

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    thrombospondin related sporozoite protein (TRSP) (PF3D7_0104000 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. PF3D7_0910300-COMP-blac-flag-his

    Plasmid

    #110959

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    conserved Plasmodium protein, unknown function (PF3D7_0910300 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. PF3D7_1242000-COMP-blac-flag-his

    Plasmid

    #111025

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    conserved Plasmodium protein (PF3D7_1242000 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge