1,927 results
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Plasmid#66824PurposeYPET^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertYPET-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized YPETExpressionWormAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJA58
Plasmid#59933PurposegRNA for cleavage at dpy-10(cn64) locus in C elegansDepositorInsertdpy-10(cn64) gRNA
UseCRISPRPromoterU6Available SinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLT 651
Plasmid#59998PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorExpressionBacterialMutationpartial genomicAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ212 - pDESTR4-R3(cxTi10816, IV)
Plasmid#34864DepositorInsertcxTi10816 targeting
UseWorm targetingAvailable SinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pAD43-daf-16 RNAi
Plasmid#34833DepositorAvailable SinceJune 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)
Plasmid#107939Purposepie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.DepositorInsertmCherry(dpiRNA)::ANI-1(AHPH)
ExpressionWormPromoterpie-1Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPK605
Plasmid#38148DepositorInsertsunc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR) (unc-119 Nematode)
pie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR
ExpressionWormPromoterpie-1 promoter and unc-119Available SinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
Punc-4::NGL-1::spGFP1-10
Plasmid#65827PurposeFor trans-synaptic labelingDepositorAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDYH5
Plasmid#52032PurposeExpression of GFP-tagged DAF-16 in C. elegansDepositorAvailable SinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
dg265_pENTR_CoChRCel
Plasmid#66103PurposeEntry clone containing CoChR coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2DepositorInsertCoChR
UseGateway CloningAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1310
Plasmid#61253PurposeTemplate for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusionDepositorInsertU6 promoter
UseCRISPR; Cloning templateAvailable SinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDD285
Plasmid#66826PurposemKate2^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmKate2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized mKate2ExpressionWormAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP
Plasmid#24033DepositorAvailable SinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDD315 (mTurquoise2^SEC^2xHA)
Plasmid#73343PurposemTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology armsDepositorInsertmTurquoise2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags2xHA and C. elegans codon-optimized mTurquoise2ExpressionWormAvailable SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJR031
Plasmid#198825PurposeStable step function ChR2 variant. Activated by bluelight, terminated by green light.DepositorInsert15xUAS::ChR2(C128S)-EYFP::let-858 3'UTR
ExpressionWormAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJJR83
Plasmid#75028PurposemCherry^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmCherry^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized mCherryExpressionWormAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_RGG-GFP-RGG
Plasmid#124930PurposeExpression of RGG-GFP-RGGDepositorInsertRGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsGFPExpressionMammalianPromoterCMVAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNM3823
Plasmid#154123Purposemec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vectorDepositorInsertmec-4p::tetR-L-QFAD::act-4 '3
UseRmceExpressionWormPromoternot applicableAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
Plasmid#159849PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM228 - [Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR]
Plasmid#159895PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR
ExpressionWormAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBN42
Plasmid#86713PurposeC. elegans injection markerDepositorInsertPmyo-3::GFP
ExpressionWormAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr
Plasmid#19326DepositorArticleInsertPmyo-3::twk-18(cn110)cDNA
ExpressionWormAvailable SinceOct. 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
JH01
Plasmid#73510PurposeGFPo_PH pCFJ150 - contains C. elegans sequence optimized GFPDepositorInsertpmex-5::GFPo::PH::tbb-2
TagsGFPPromotermex-5Available SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]
Plasmid#159808PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-B
Plasmid#177785PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCM1.36
Plasmid#17249DepositorInserttbb-2 3'UTR in pDONRP2RP3
UseGateway CloningAvailable SinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3163
Plasmid#101248PurposeGFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.DepositorAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET_MBP-RGG-GFP-RGG
Plasmid#124949PurposeExpression of MBP-RGG-GFP-RGGDepositorInsertMBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsEGFP, His, and MBPExpressionBacterialAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNM3689
Plasmid#154111PurposeminiMos single component RMCE landing site vectorDepositorInsertloxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
UseMinimosPromoternot applicableAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only