Skip to main content
Addgene
Showing: 301 - 350 of 612 results

  5. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

    Plasmid
    #128604
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Aug. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    128604-plasmid-map-sequence-id-457471

    Enlarge

  14. 12xMoonTag-12xSunTag-kif18b-24xPP7

    Plasmid
    #128606
    Purpose
    Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    12xMoonTag-12xSunTag
    kinesin family member 18b (KIF18B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128606-plasmid-map-sequence-id-254156

    Enlarge

  20. pAAV-DYRK1A-2A-mCherry

    Plasmid
    #118272
    Purpose
    Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV with beta global intron
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118272-plasmid-map-sequence-id-229471

    Enlarge

  26. pAAV-DYRK1A-2A-GFP

    Plasmid
    #118270
    Purpose
    Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
    Use
    AAV
    Tags
    2A peptide and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV with beta global intron
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118270-plasmid-map-sequence-id-229494

    Enlarge

  42. pAAV-DCX(S306D)-2A-mCherry

    Plasmid
    #118292
    Purpose
    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acid
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    Doublecortin (DCX Human)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Mutation
    Serine 306 changed to Aspartic Acid
    Promoter
    CMV with beta global intron
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118292-plasmid-map-sequence-id-229506

    Enlarge

  43. pAAV-DCX(S306A)-2A-mCherry

    Plasmid
    #118291
    Purpose
    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanine
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    Doublecortin (DCX Human)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Mutation
    changed Serine 306 to Alanine
    Promoter
    CMV with beta global intron
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118291-plasmid-map-sequence-id-229504

    Enlarge

  44. Negative Control Binder

    Plasmid
    #179140
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptide
    Depositor
    Klaus Hahn
    Article
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Use
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9. Addi…
    Promoter
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179140-plasmid-map-sequence-id-353410

    Enlarge

Showing: 301 - 350 of 612 results