Addgene

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pCellFree_G03 STAT6

Plasmid

#67123

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT6 (STAT6 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pSuper-TatSF1 GH

Plasmid

#31120

Depositor

Mariano Garcia-Blanco

Article

Miller et al PLoS One. 2009 . 4(5):e5710.

Insert

TatSF1 shRNA (HTATSF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pSuper-TatSF1 AB

Plasmid

#31119

Depositor

Mariano Garcia-Blanco

Article

Miller et al PLoS One. 2009 . 4(5):e5710.

Insert

TatSF1 shRNA (HTATSF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
Stat92e pBluescript SK(+)

Plasmid

#8712

Depositor

Jim Darnell

Article

Yan et al Cell 1996 Feb 9;84(3):421-30.

Insert

Stat92e (Stat92E Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2005

Availability

Academic Institutions and Nonprofits only
pUASTattB-cytoFLARE1.0-TF

Plasmid

#234521

Purpose

Transcriptional reporter for detecting calcium transients in Drosophila larvae (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al bioRxiv [Preprint]. 2025 Jan 18:2025.01.

Insert

ERT2-MK2-hLOV-TEVcs-FLAG-LexA-VP16

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pUASTattB-degradALFA

Plasmid

#219663

Purpose

Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

degradALFA

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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WDV-Echistatin

Plasmid

#226985

Purpose

Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

Depositor

Wendy Gordon

Article

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Inserts

WDV (repA )
Echistatin

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p1X-UASTattB

Plasmid

#189861

Purpose

Fly cloning and transformation

Depositor

Jung Hwan Kim

Article

Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-Mcl1_deltaTM

Plasmid

#187118

Purpose

insect cell expression of Mcl1-deltaTM

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

MCL-1 (BCL2 Human)

Use

Tags

Strep

Expression

Insect

Mutation

delta328-350

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-GTATT

Plasmid

#170099

Purpose

deGFP reporter plasmid with GTATT PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRcCMV-STAT31F

Plasmid

#134725

Purpose

Expresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707

Depositor

Curt Horvath, Jim Darnell

Article

Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Insert

STAT3-STAT1 Fusion (STAT3, STAT1 Human)

Use

Tags

FLAG and human STAT3 aa 1-514 joined to human STA…

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRcCMV-STAT13F

Plasmid

#134723

Purpose

Expresses chimeric STAT1-STAT3 protein with human STAT1 amino acids 1-508 joined to human STAT3 at amino acid 515, with a FLAG epitope tag at the C-terminus. Based on addgene plasmids #8691 and #8707

Depositor

Curt Horvath, Jim Darnell

Article

Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Insert

STAT1-STAT3 fusion (STAT3, STAT1 Human)

Use

Tags

FLAG and human STAT1 aa1-508 joined to human STAT…

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGDP1 stat

Plasmid

#112886

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

stat

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Pbla

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pVL12 - tAt4g24550

Plasmid

#116032

Purpose

entry vector for GreenGate cloning method, terminator

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

tAt4g24550

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 STAT1

Plasmid

#67116

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT1 (STAT1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pUASTattb AaegOr4-G

Plasmid

#59798

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele G

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele G

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-F

Plasmid

#59797

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele F

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele F

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASTattb AaegOr4-D

Plasmid

#59795

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele D

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele D

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASTattb AaegOr4-B

Plasmid

#59793

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele B

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele B

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
III-ATAT

Plasmid

#43526

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

III-ATAT

Use

Bacterial cloning

Tags

Expression

Mutation

Promoter

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only