We narrowed to 18,373 results for: Met

1. pETSUMO2_Bacteroidetes-bGSDM

   Plasmid

   #212124

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Bacteroidetes bGSDM
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS313_ATP3

   Plasmid

   #120253

   Purpose

   Expression of ATP3 in yeast

   Depositor

   Markus Ralser

   Article

   Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

   Insert

   ATP3 (ATP3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ATP3

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH4504

   Plasmid

   #191702

   Purpose

   Pnmr-1 long::TeTx::wCherry unc-54 3' UTR C.elegans AVA/other neurons expression of TeTx RFP

   Depositor

   Mei Zhen

   Article

   Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

   Insert

   Tetanus Toxin Light Chain
   

   Use

   Tags

   wCherry

   Expression

   Worm

   Mutation

   Promoter

   Pnmr-1 long

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSKA397

   Plasmid

   #80380

   Purpose

   mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

   Depositor

   Mustafa Khammash

   Article

   Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

   Inserts

   cat
   metE
   

   Use

   Synthetic Biology; Mini-tn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cat promoter and cpcG2Δ59

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH-eCFP

   Plasmid

   #176555

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of histidine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pL-eCFP

   Plasmid

   #176556

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of leucine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU-eCFP

   Plasmid

   #176557

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of uracil

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU-mRuby2

   Plasmid

   #176549

   Purpose

   Expression of mRuby2 for auxotrophic selection in the absence of uracil

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   yomRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH-mRuby2

   Plasmid

   #176547

   Purpose

   Expression of mRuby2 for auxotrophic selection in the absence of histidine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   yomRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

    Plasmid

    #84260

    Purpose

    Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgSV40
    PYL1-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and mouse U6

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-synp-F-H2B-GCaMP6f

    Plasmid

    #102994

    Purpose

    Expresses histone fused GCaMP6f under the synapsin promoter

    Depositor

    David Cox

    Article

    Skocek et al Nat Methods. 2018 Jun;15(6):429-432. doi

    Insert

    H2B-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xMx PylT C41CA_GFP 150TAG

    Plasmid

    #140017

    Purpose

    Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p35S::IRE1a

    Plasmid

    #135232

    Purpose

    constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    IRE1a (IRE1A Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pECE-HA-FOXO3 R248K R250K

    Plasmid

    #52427

    Purpose

    mammalian expression of FOXO3 methylation resistant mutant

    Depositor

    Steven Weinman

    Article

    Tikhanovich et al Hepatology. 2014 Jan;59(1):58-70. doi: 1

    Insert

    FOXO3a (FOXO3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R248K R250K, mutant methylation sites

    Promoter

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. p35S::HY5

    Plasmid

    #135233

    Purpose

    constitutive expression of HY5 from A. thaliana (AT5G11260; downregulates UPR signaling)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    HY5 (HY5 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-hRPL3-FLAG_WT

    Plasmid

    #233616

    Purpose

    Expression of Flag-tagged hRPL3

    Depositor

    Shintaro Iwasaki

    Article

    Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

    Insert

    Flag-tagged hRPL3 (RPL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-hRPL3-FLAG_H245A

    Plasmid

    #233617

    Purpose

    Expression of Flag-tagged hRPL3 with H245A mutation

    Depositor

    Shintaro Iwasaki

    Article

    Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

    Insert

    Flag-tagged hRPL3 with H245A mutation (RPL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H245A

    Promoter

    Available Since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG94-9

    Plasmid

    #228968

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-9
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaG94-10

    Plasmid

    #228969

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-10
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21-pelB-LaG94-12

    Plasmid

    #228970

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-12, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-12
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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