We narrowed to 9,586 results for: Coli
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Plasmid#72943PurposeLevel 1 Destination vector - Position D1DepositorInsertLevel 1 - D1 site
UseSynthetic BiologyMutationBeta-lactamase - G717C (nucleotide) - silent muta…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-E-RFP
Plasmid#72944PurposeLevel 1 Destination vector - Position EDepositorInsertLevel 1 - E site
UseSynthetic BiologyMutationBeta-lactamase - G717C (nucleotide) - silent muta…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF-eGFP
Plasmid#72960PurposeLevel 0 Entry vector" ORF - eGFP "DepositorInsertEnhanced green flourescent protein (eGFP)
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-Pl-lacO1-deGFP-T500 (123p)
Plasmid#70199PurposePromoter panelDepositorInsertdeGFP
Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23113-deGFP-T500 (124p)
Plasmid#70200PurposePromoter panelDepositorInsertdeGFP
Available SinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-pNull-deGFP-T500 (133p)
Plasmid#70209PurposePromoter panelDepositorInsertdeGFP
Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23102-deGFP-T500 (132p)
Plasmid#70208PurposePromoter panelDepositorInsertdeGFP
Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23100-deGFP-T500 (130p)
Plasmid#70206PurposePromoter panelDepositorInsertdeGFP
Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23151-deGFP-T500 (129p)
Plasmid#70205PurposePromoter panelDepositorInsertdeGFP
Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23106-deGFP-T500 (128p)
Plasmid#70204PurposePromoter panelDepositorInsertdeGFP
Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23150-deGFP-T500 (127p)
Plasmid#70203PurposePromoter panelDepositorInsertdeGFP
Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23116-deGFP-T500 (126p)
Plasmid#70202PurposePromoter panelDepositorInsertdeGFP
Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23114-deGFP-T500 (125p)
Plasmid#70201PurposePromoter panelDepositorInsertdeGFP
Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK591
Plasmid#71589PurposeProduces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)DepositorInsertacetyl-CoA:oxalate CoA-transferase
ExpressionBacterialMutationchanges threonine-2 to alanine and aspartate-177 …PromoterT7Available SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVK_FG
Plasmid#66070PurposeMoClo Destination Vector: (based on pSB1K3) [F:LacZa:G]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVK_GH
Plasmid#66071PurposeMoClo Destination Vector: (based on pSB1K3) [G:LacZa:H]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
SepOTSι
Plasmid#68290PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EFSep21, 2xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSη
Plasmid#68288PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EF-Sep21, 2xtRNA-Sep
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSζ
Plasmid#68287PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 2xtRNA-Sep
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSδ
Plasmid#68285PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 1xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5woH
Plasmid#65019PurposeExpresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureusDepositorInsertsortase A
TagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1148
Plasmid#53006PurposepCon:OxyS, pLlacO-1:sodB::gfpDepositorInsertsOxyS sRNA
sodB
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon and pLlacO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1057
Plasmid#53001PurposepCon:RhyB, pLlacO-1:rpoS::gfpDepositorInsertsRhyB sRNA
rpoS
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontainsn −150 to +30 bp relative to start codonPromoterpCon and pLlacO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1561
Plasmid#37571DepositorAvailable SinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL1559
Plasmid#37570DepositorAvailable SinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL1324
Plasmid#37638DepositorInsertsGFP
TetR
CsrA
UseSynthetic BiologyExpressionBacterialPromoterpCon and pConNoHindAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 3076 (=HL 716 + pHL 1069)
Plasmid#30036DepositorInsertpCon:MicC, pLlacO-1:sodB::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialAvailable SinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4179 (=HL 865 + pHL 1381)
Plasmid#30098DepositorInsertpLlacO-1:hfq, pLtetO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialAvailable SinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4202 (=HL 716 + pHL 1389)
Plasmid#30100DepositorInsertpLtetO-1:DsrA, pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialAvailable SinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4178 (=HL 862 + pHL 1360)
Plasmid#30097DepositorInsertpLlacO-1:hfq, pLtetO-1:ompC::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialAvailable SinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSL5 SMR1713
Plasmid#28028DepositorInsertMutL (mutL E. coli)
ExpressionBacterialAvailable SinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII282Pglu-4m
Plasmid#13564DepositorInsertFLIPglu F16A T282S-ECFP-N283R
TagsCitrineExpressionBacterialMutationF16A, ECFP insert in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pESdelta 799
Plasmid#13042DepositorInsertMutS
TagsHis6ExpressionBacterialMutationC-terminal deletion from aa 800 through 853.Available SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
Cytoplasmic PancACe
Plasmid#215708PurposeExpresses acetyl-CoA biosensor in the cytoplasmDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and cytoplasmic localization sequenceExpressionMammalianPromoterCMVAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Nuclear PancACe
Plasmid#215706PurposeExpresses acetyl-CoA biosensor in the nucleusDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and NLSExpressionMammalianPromoterCMVAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mitochondrial PancACe
Plasmid#215710PurposeExpresses acetyl-CoA biosensor in the mitochondriaDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianPromoterCMVAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseExpressionBacterialPromoternative promoterAvailable SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas
Plasmid#62225PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRDepositorHas ServiceCloning Grade DNAInsertcas9
UseCRISPR; Lambda red recombinaseExpressionBacterialPromoternative cas9 promoterAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)
Plasmid#70210Purpose1 Part of 4-part switchDepositorInsertdeGFP
Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Alive
Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
TagsHisExpressionBacterialMutationWt geneAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Dead
Plasmid#20859PurposeDead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).DepositorInsertStreptavidin-Dead
ExpressionBacterialMutationTriple mutant of wt Streptavidin N23A,S27D,S45AAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
LM670
Plasmid#191552PurposeLM670_MSG-AGGA-promoter-ccdb-TTCC-GShisDepositorInsertccdb
TagsHIS-tagExpressionBacterialAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
aav-CAG-tdNfsB(F124W)-mCherry (eNTR)
Plasmid#113761PurposeAAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoterDepositorInserttdNfsB(F124W)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationF124WPromoterCAGAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGM1190
Plasmid#69994PurposeOriT containing mobilizable vector for thiostrepton induced gene expression in StreptomycesDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
ExpressionBacterialAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidExpressionBacterialAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTP264
Plasmid#149334PurposeBacterial expression of Biotin ligase BirADepositorInsertBiotin ligase BirA
TagsHis14 and bdNEDD8ExpressionBacterialAvailable SinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-2 sfGFP
Plasmid#154464PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPBCAG-rtTAM2-IH
Plasmid#140827PurposeExpresses rtTAM2-IRES-HygR in mammalian cellsDepositorInsertsrtTAM2
HygR
ExpressionMammalianAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PsppA-mCherry
Plasmid#225428PurposePlasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherryDepositorInsertmCherry, Cas9, SgRNA
UseCRISPRExpressionBacterialPromoterPsppA upstream of mCherry and Cas9, P3 upstream o…Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 rmtB
Plasmid#112880PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only