We narrowed to 4,881 results for: phy

1. LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine

   Plasmid

   #104122

   Purpose

   synOptopatch construct

   Depositor

   Adam Cohen

   Article

   Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

   Insert

   spine-jRGECO1a-P2A-QuasAr-dark Citrine
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MAC_C_LMTK2

   Plasmid

   #187752

   Purpose

   MAC-tagged gene expression of human LMTK2

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   LMTK2 (LMTK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. POLArIS_NbVim-cp10GFP

   Plasmid

   #171020

   Purpose

   Expresses NbVim-cp10GFP#2 in mammalian cells

   Depositor

   Sumio Terada

   Article

   Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

   Insert

   anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
   

   Use

   Tags

   cp10GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. attB-­GFP-­Poly(A)

   Plasmid

   #65524

   Purpose

   Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-GFP
   

   Use

   Mouse Targeting; Bxb1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MAC_C_IGF1R

   Plasmid

   #187770

   Purpose

   MAC-tagged gene expression of human IGF1R

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   IGF1R (IGF1R Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFUGW-RapACR-EYFP

   Plasmid

   #111075

   Purpose

   Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RapACR
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZeo-Cas9-gate

   Plasmid

   #113744

   Purpose

   Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   Cas9
   

   Use

   CRISPR; Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Z. Mays ubiquitin promoter, 35S promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQmod2S-GG

   Plasmid

   #191346

   Purpose

   Clostridium expression vector (pBP1 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   Plac-RFP (IPTG-inducible red fluorescent protein)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFUGW-RapACR_T111C-EYFP

   Plasmid

   #119195

   Purpose

   Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RapACR fast mutant T111C
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   changed threonine 111 to cysteine

   Promoter

   Ubiquitin

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR-D-TOPO-kcnn4

    Plasmid

    #164960

    Purpose

    Zebrafish kcnn4 gene CDS

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

    Insert

    kcnn4 (kcnn4 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ROZA-XL

    Plasmid

    #64194

    Purpose

    Fluorescent reporter for ZAP-70 tyrosine kinase activity

    Depositor

    Annemarie Lellouch

    Article

    Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.

    Insert

    ROZA-XL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ROZA-XL

    Promoter

    CMV

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pTYF-super-mfvAChT-1.8k-Venus-WPRE

    Plasmid

    #156389

    Purpose

    Expresses Venus under vAChT gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-HA-ERK2 L198A L232A

    Plasmid

    #8978

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L198A, L232A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pY017 (pcDNA3.1-hPcCpf1)

    Plasmid

    #69989

    Purpose

    Expresses humanized PcCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPcCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA6-LRRC55-V5-Flag-His

    Plasmid

    #127819

    Purpose

    Expression of human LRRC55 in mammalian cells

    Depositor

    Jiusheng Yan

    Article

    Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

    Insert

    LRRC55 (LRRC55 Human)
    

    Use

    Tags

    V5, FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pP19 (GB0038)

    Plasmid

    #68195

    Purpose

    Provides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    P19 coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pgWIZ-SGCAgl

    Plasmid

    #131456

    Purpose

    Encodes Alpha Sarcoglycan 3xFLAG tagged protein (Therapeutic gene) to cure LGMD2D and luciferase enzyme for in vivo live imaging

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    SGCA (SGCA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPKm-202

    Plasmid

    #90492

    Purpose

    Expresses pcDNA3 - CMVmin - 5X UAS - pFR - CMVmin Luciferase

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    CMVmin Luciferase Fluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Plasmid

    #163074

    Purpose

    Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP1-iEER
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SmtA Synechococcus elongatus PCC 7942 Metallothionein

    Plasmid

    #200298

    Purpose

    Bacterial expression plasmids for production of recombinant Synechococcus elongatus PCC 7942 Metallothionein

    Depositor

    Artur Krężel

    Article

    Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

    Insert

    Gene of metallothionein with Chiting Binding Domanin-Intein (SYNPCC7942_RS13750 Synechococcus elongatus)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRKB134_GI-DUX4-intron2(-)

    Plasmid

    #83968

    Purpose

    to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

    Depositor

    Robert Bradley

    Article

    Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

    Insert

    DUX4-3'UTR-intron2(-) (DUX4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouseCMV

    Available since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

22. pRKB133_GI-DUX4-intron2(+)

    Plasmid

    #83967

    Purpose

    to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

    Depositor

    Robert Bradley

    Article

    Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

    Insert

    DUX4-3'UTR-intron2(+) (DUX4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouseCMV

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pAL190

    Plasmid

    #22279

    Purpose

    Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    TimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pU6-26 (GB1001)

    Plasmid

    #68208

    Purpose

    Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtU6-26 promoter
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJRTCLCrVA.008

    Plasmid

    #22841

    Depositor

    Niki Robertson

    Article

    Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

    Insert

    A DNA (with MCS)
    

    Use

    Cotton vigs vector

    Tags

    Expression

    Bacterial

    Mutation

    Coat Protein (AR1 gene) of Cotton Leaf Crumple Vi…

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHsp18.2 (GB0153)

    Plasmid

    #68171

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp18.2 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-HsSENP3(307-574)

    Plasmid

    #71481

    Purpose

    Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 3 (SENP3 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-306 (catalytic domain only)

    Promoter

    T7

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pCAG-T3-st1CAS9-pA

    Plasmid

    #131542

    Purpose

    Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

    Insert

    Codon optimized st1Cas9
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGB Tnos:NptII:Pnos (GB0226)

    Plasmid

    #68212

    Purpose

    Transcriptional unit for kanamycin resistance gene plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    NptII
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MTK3_009

    Plasmid

    #123724

    Purpose

    Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PHYB-VP16-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Dronpa-Mito-7

    Plasmid

    #57288

    Purpose

    Localization: Mitochondria, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    Dronpa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

32. pEGFPC1/GgVcl 1-258 A50I mutation

    Plasmid

    #46271

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-258 (aka VD1) A50I mutation (VCL Chicken)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    1-258 (VD1 domain) A50I mutation

    Promoter

    CMV

    Available since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTRE-FDXR wt

    Plasmid

    #16610

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    FDXR (FDXR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mEos2-Beta-Catenin-20

    Plasmid

    #57350

    Purpose

    Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Beta-Catenin (Ctnnb1 Mouse)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pHsp70B (GB0155)

    Plasmid

    #68172

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp70B promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDelila (GB0079)

    Plasmid

    #68200

    Purpose

    Provides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Delila coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRosea (GB0026)

    Plasmid

    #68194

    Purpose

    Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    A. majus Rosea coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPKm-102

    Plasmid

    #90493

    Purpose

    pcDNA3 - CMVmin - mOrange, plasmid expressing orange fluorescent protein (OFP)

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    mOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGP8GF-AtTIR1

    Plasmid

    #188427

    Purpose

    Arabidopsis Nuclear Auxin Signaling Receptor TIR1

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA6-LRRC38-V5-His

    Plasmid

    #127820

    Purpose

    Expression of human LRRC38 in mammalian cells

    Depositor

    Jiusheng Yan

    Article

    Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

    Insert

    LRRC38 (LRRC38 Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge