We narrowed to 9,389 results for: Coli

1. pHelper_W

   Plasmid

   #173784

   Purpose

   The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   Wphi Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHelper_D

   Plasmid

   #173781

   Purpose

   The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   D145 Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGR-ECK120015170

   Plasmid

   #46006

   Depositor

   Christopher Voigt

   Article

   Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

   Insert

   ECK120015170 Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p15aC-4D1D

   Plasmid

   #107864

   Purpose

   Bacterial expression of PIS and PI4K

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Inserts

   PIS
   phosphatidylinositol 4-kinase β
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   proD

   Available since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TDGS50

   Plasmid

   #183189

   Purpose

   TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

   Depositor

   Hsiao-Chun Huang

   Article

   Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

   Inserts

   DivIVA-GFP(tevR M0050)
   TEV-S219V
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKS043

   Plasmid

   #65471

   Purpose

   pTrc plasmid expressing clpS

   Depositor

   Keith Tyo

   Article

   Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

   Insert

   clpS (clpS )
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSELt_S7.A

   Plasmid

   #125835

   Purpose

   Negative selection plasmid for evolution of an amber stop codon suppression tRNA

   Depositor

   Andrew Ellington

   Article

   Maranhao et al ACS Synth Biol. 2017 Jan 20;6(1):108-119

   Insert

   ACP-2xAmb-pheS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pr.trpL*

   Available since

   May 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pZH509

   Plasmid

   #102664

   Purpose

   Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAK02

   Plasmid

   #19001

   Depositor

   Jay Keasling

   Article

   Khlebnikov et al J Bacteriol. 2000 Dec . 182(24):7029-34.

   Insert

   arabinose transporter araE under control of Ptac (araE E. coli)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pBP-TL4

    Plasmid

    #72988

    Purpose

    RBS_H5

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    H5 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pBP-TL8

    Plasmid

    #72992

    Purpose

    RBS_L1

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    L1 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pBP-L1U1H09

    Plasmid

    #73008

    Purpose

    L1U1H09 terminator

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    L1U1H09 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3XFlag-YedK

    Plasmid

    #84567

    Purpose

    Expresses YedK in mammalian cells

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    YedK
    

    Use

    Tags

    3xFlag and C-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEL1324

    Plasmid

    #139874

    Purpose

    Expression plasmid for WT LetB (without TM region) for cryo and negative stain EM

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGR-ECK120010783

    Plasmid

    #46009

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Insert

    ECK120010783 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDUET-ctCPR-nAMO

    Plasmid

    #20110

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and native Amorphadiene oxidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pGR-ECK125108723

    Plasmid

    #46008

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Insert

    ECK125108723 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSB1A3-AD010

    Plasmid

    #116851

    Purpose

    Expresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010

    Depositor

    Friedrich Simmel

    Article

    Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

    Inserts

    AraC
    alpha-HL
    mScarletI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKS012

    Plasmid

    #65465

    Purpose

    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherry

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    Ntag-mCherry
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pl-TetO

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pSaI14

    Plasmid

    #8614

    Depositor

    Robert Hartley

    Article

    Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

    Insert

    Ribonuclease inhibitor SaI14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N terminal threonine changed to alanine

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pTH848-gst-disec

    Plasmid

    #89589

    Purpose

    Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase codon-dis-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDF-EcAvs4

    Plasmid

    #188806

    Purpose

    Codon optimized EcAvs4 with C-terminal His tag

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    EcAvs4
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZH512

    Plasmid

    #102666

    Purpose

    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGR-ECK120010800

    Plasmid

    #46010

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Insert

    ECK120010800 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDUET-ctCPR-BovAMO

    Plasmid

    #20113

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase, and amorphadiene oxidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pKB234.2

    Plasmid

    #170023

    Purpose

    Encodes LacI-controlled expression of surface-displayed hBD3 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    hBD3 Δ alpha-helix
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    Dec. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pES E38A

    Plasmid

    #13049

    Depositor

    Peggy Hsieh

    Article

    Schofield et al J Biol Chem. 2001 Dec 7. 276(49):45505-8

    Insert

    MutS
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    mutation Glu38 to Ala

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pGC4780

    Plasmid

    #113408

    Purpose

    Translation reporter with inducible translation coupling (high native GFP expression)

    Depositor

    Guillaume Cambray, Adam Arkin

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Insert

    158_33121123_1
    

    Use

    Synthetic Biology

    Tags

    fused to codon deptimized sfGFP with Flexible lin…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDUET-ctCPR-sAMO

    Plasmid

    #20111

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and codon optimized amorphadiene oxidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pZH511

    Plasmid

    #102665

    Purpose

    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a-ClbN-NHis

    Plasmid

    #51497

    Purpose

    NRPS module containing CATE domains with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbN
    

    Use

    Tags

    6x His tag and T7 tag

    Expression

    Bacterial

    Mutation

    contains aa30-1094 of NP_754347.1

    Promoter

    T7

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDUET-ctCPR-A13AMO

    Plasmid

    #20114

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and amorphadiene oxidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

33. pEc-crRNA1

    Plasmid

    #170088

    Purpose

    encodes E. coli type I-E single spacer array

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    E. coli type I-E single spacer array
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDUET-ctCPR-trAMO

    Plasmid

    #20112

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and truncated amorphadiene oxidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA5/FRT/TO GFP DNAK

    Plasmid

    #19490

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    dnaK molecular chaperone DnaK
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSaI20

    Plasmid

    #8615

    Depositor

    Robert Hartley

    Article

    Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

    Insert

    Ribonuclease inhibitor SaI20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N terminal threnine changed to alanine

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. circRAJ31-Toehold_mutant

    Plasmid

    #69930

    Purpose

    Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.

    Depositor

    Alfonso Jaramillo

    Article

    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

    Insert

    plTet-circRAJ31_toeholdmutant
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    4 bases mutated in toehold of riboregulator

    Promoter

    PltetO1-2

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTEI122

    Plasmid

    #126062

    Purpose

    Module 0 vector of PlaMiNGo toolkit

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    ccdB Cassette
    

    Use

    Synthetic Biology; Module 0

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBP-TL9

    Plasmid

    #74098

    Purpose

    TL9 RBS (AAAAAG)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    TL9 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5/FRT/TO HIS DNAK

    Plasmid

    #19544

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    dnaK molecular chaperone DnaK
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge