We narrowed to 15,547 results for: pET
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Plasmid#141322PurposePlasmid for bacterial expression and purification of RfxCas13d proteinDepositorInsertRfx-Cas13d
UseCRISPRTags6xHisExpressionBacterialPromoterT7Available SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP2 (catalytic domain)
Plasmid#16357DepositorInserthuman SENP2 catalytic domain (SENP2 Human)
TagsHisExpressionBacterialMutationAmino Acid 365 to 590 catalytic domain of human S…Available SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET14b His Rab8a Q67L
Plasmid#186014PurposeHis Rab8a Q67LDepositorInsertHis Rab8a Q67L (RAB8A Human)
ExpressionBacterialAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-AsCas12f-2NLS
Plasmid#171613PurposeNLS-AsCas12f1-NLS heterologous expression plasmid for RNPDepositorInsertNLS-AsCas12f1-NLS
ExpressionBacterialAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)
Plasmid#205049PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)
TagsMGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-2 sfGFP
Plasmid#154464PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-Cas9-His
Plasmid#47327PurposeFor in vitro expression and purification of Cas9 proteinDepositorInsertCas9
UseCRISPRTagsHisExpressionBacterialAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-His-TEV-NQO1
Plasmid#119058PurposeFor expression in E.coli and affinity purification of N-terminally His tagged NQO1DepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
Tags6His, TEV siteExpressionBacterialPromoterT7Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-Cas9-NLS-6xHis
Plasmid#62933PurposeExpression of Cas9-NLS-6xHis in bacterial cellsDepositorInsertCas9-NLS-6xHis
Tags6xHis tag and NLSExpressionBacterialPromoterT7Available SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-ARH3-His-Sumo-HA
Plasmid#111578PurposeExpresses ARH3 (amino acids 14-363) in E. coliDepositorInsertARH3 (ADPRS Human)
Tags6xHis-Sumo and HAExpressionBacterialMutationdeleted amino acids 1-13PromoterT7Available SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-NLS-Cas9-6xHis
Plasmid#62934PurposeExpression of NLS-Cas9-6xHis in bacterial cellsDepositorInsertNLS-Cas9-6xHis
Tags6xHis tag and NLSExpressionBacterialPromoterT7Available SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP-HMGB1-WT
Plasmid#194543PurposeBacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion ProteinDepositorAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ULP1-405-621
Plasmid#229224PurposeFor expression of ULP1 proteinDepositorAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-ERK2-MEK1_R4F_coexpression
Plasmid#39212DepositorTagsHis6 and RBSExpressionBacterialMutationMEK1(R4F) = S218E, S222D and deletion of AA32-51PromoterT7 and T7 via RBSAvailable SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-His6MBP-A3A-Gly6His6
Plasmid#187822PurposeExpression human APOBEC3A in E.coli.DepositorInsertAPOBEC3A (APOBEC3A Human)
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationnonePromoterT7 promoter (on the backbone)Available SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*-XcP4H-MH4R
Plasmid#178043PurposeExpress green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTPDepositorInsertssuperfolder green fluorescent protein with TAG at 151 position
XcP4H with W179F mutation
dihydropterine reductase from human
pterin-4a-carbinolamine dehydratase from human
TagsHis tagExpressionBacterialAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop A
Plasmid#171779PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23DepositorInsertsfGFP-DogTag Loop A
TagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Val2…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-TEV-C9R
Plasmid#118754PurposeTEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activityDepositorInsertTobacco Etch Virus protease (NEWENTRY Synthetic)
TagsC9R and His tagExpressionBacterialPromoterT7Available SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29b-dspB(E184Q)-6xHis
Plasmid#175802PurposePlasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsHexahistidine tagExpressionBacterialMutationE184QPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos15)GFP
Plasmid#89248Purpose[Edit] Expresses GFP with +15 theoretical charge in E.ColiDepositorInsertHis6-(pos15)-GFP
TagsHis6ExpressionBacterialPromoterT7Available SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET23b-15F11-HA-mEGFP-6xHis
Plasmid#129593PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
Tags6xHis-tagExpressionBacterialPromoterT7Available SinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET302-6His-dCas9-Halo
Plasmid#72269PurposeEncodes a Halo-labeled fusion of nuclease deficient Cas9DepositorInsertHis6-dCas9-Halo
Available SinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos9)GFP
Plasmid#89247PurposeExpresses GFP with +9 theoretical charge in E.ColiDepositorInsertHis6-(pos9)-GFP
TagsHis6ExpressionBacterialPromoterT7Available SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-D-TOPO-PARP14-SΔK
Plasmid#216658PurposeExpresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.DepositorInsertPARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
Tags6xHis-V5-TEVExpressionBacterialMutationDeletion starting from Q1390 to D1448 and substit…PromoterT7Available SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
Tags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET-dCas9-VP64-6xHis
Plasmid#62935PurposeExpression of dCas9-VP64-6xHis in bacterial cellsDepositorInsertdCas9-VP64
Tags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A and H840A amino acid changes render Cas9 nuc…PromoterT7Available SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-His6-TEV_SacB
Plasmid#164588PurposeBacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.DepositorInsertTEV-SacB
TagsTrx-His6 followed by TEV siteExpressionBacterialPromoterT7Available SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mCherry-Cbx5
Plasmid#194547PurposeBacterial Expression of 6xHis mCherry-HP1a Fusion ProteinDepositorInsertCbx9 (HP1a)
TagsmCherryExpressionBacterialAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RNA Ligase
Plasmid#119941PurposeExpress TS2126 RNA ligase in E. coliDepositorInsertTS2126 RNA ligase
TagsHis6ExpressionBacterialPromoterT7Available SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pet16b ALIX Bro1 (1-359)
Plasmid#80641PurposeResidues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428DepositorInsertALIX Bro1, residues 1-359 (PDCD6IP Human)
TagsHis TagExpressionBacterialMutationResidues 1-359PromoterT7Available SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pet28a-His-tag-BS2
Plasmid#220347PurposeE. coli expression vector to purify BS2DepositorInsertBS2
TagsHis tagExpressionBacterialAvailable SinceJune 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7Available SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HypaSpCas9-NLS-6xHis
Plasmid#207386PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HypaSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN692A, M694A, Q695A, H698A, amino acids 1005-1013…PromoterT7Available SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
1a. pETM11-SUMO-SNCA-GFP
Plasmid#107292PurposePlasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-HaloTag7
Plasmid#167266PurposeOverexpression of HaloTag7 in E. coliDepositorInsertHaloTag7
TagsHis x10ExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132)
Plasmid#73742PurposeExpression of human RPN13 PRU domain in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationPRU domain only (residues 19-132)PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_NSP4ss_SerM_NucA-myc
Plasmid#218191PurposeBacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)DepositorArticleInsertnucA
TagsNSP4 signal secretion peptide and mycExpressionBacterialMutationcodon optimized ecoli residues - 22-266PromoterT7Available SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-HLA-A0201-ST3
Plasmid#223220PurposeExpression of human HLA A2 heavy chain fused to Spytag003 for expression in bacteriaDepositorAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB3-mCherry-6xHis
Plasmid#130985PurposeBacterial expression of mouse EB3-mCherry-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only