Skip to main content
Addgene

We narrowed to 8,865 results for: BLI;

Showing: 3751 - 3800 of 8865 results
  1. pAAV-TRE-TdTomato

    Plasmid
    #110338
    Purpose
    TdTomato reporter vector of iTango and Cal-Light system for AAV production
    Depositor
    Insert
    tetO-TdTomato
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.3_SARS2_BA.2.86

    Plasmid
    #208441
    Purpose
    expressing SARS-CoV-2 BA.2.86 spike protein for pseudovirus production
    Depositor
    Insert
    Spike (BA.2.86) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    see GISAID sequence
    Promoter
    CMV
    Available since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pSNAP-CaaX Control Plasmid

    Plasmid
    #101133
    Purpose
    Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells
    Insert
    CaaX Box
    Use
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. pET28a-mOct4 Full length(FL)

    Plasmid
    #206394
    Purpose
    Expresses Oct4 with a 6xHis tag for protein purification
    Depositor
    Insert
    Oct4 (Pou5f1 Mouse)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pGFP FAK Y397F

    Plasmid
    #50516
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Insert
    protein tyrosine kinase, mutated
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Y397F
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pQCXIP-INHBA

    Plasmid
    #73015
    Purpose
    Retroviral expression of human activin A
    Depositor
    Insert
    INHBA (INHBA Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. pZDonor rosa26 LSL-mMALAT1

    Plasmid
    #140691
    Purpose
    generate mouse MALAT1 conditional transgenic mice using CRISPR system targeting mouse Rosa 26 region
    Depositor
    Insert
    mouse MALAT1 (Malat1 Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pFLAG-6a-Ataxin3Q80

    Plasmid
    #22129
    Depositor
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 21, 2009
    Availability
    Academic Institutions and Nonprofits only
  9. pSK-mPar1

    Plasmid
    #53227
    Purpose
    bacterial expression of mouse Par1
    Depositor
    Insert
    Par1 (F2r Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 14, 2014
    Availability
    Academic Institutions and Nonprofits only
  10. SETD7

    Plasmid
    #40746
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    SETD7 (SETD7 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 14, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. His-RIAM

    Plasmid
    #32803
    Depositor
    Insert
    RIAM (APBB1IP Human)
    Use
    Tags
    His and XPress
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Nov. 18, 2011
    Availability
    Academic Institutions and Nonprofits only
  12. pScarlessHD-2xHA-DsRed

    Plasmid
    #80822
    Purpose
    Vector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Insert
    2xHA-piggyBac-DsRed
    Use
    Unspecified
    Tags
    2xHA
    Expression
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. pET28a-HIV p24

    Plasmid
    #212195
    Purpose
    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus
    Depositor
    Insert
    Chain A, Capsid protein p24, HIV type 1
    Use
    Tags
    Polyhistidine
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. alpha5-EGFP

    Plasmid
    #118956
    Purpose
    GABAA receptor expression (alpha5 subunit). EGFP tag at the C-terminus.
    Depositor
    Insert
    GABRA5 (Gabra5 Rat)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. pCGN-MEF2D

    Plasmid
    #32963
    Purpose
    For mammalian expression of mouse MEF2D with an HA tag
    Depositor
    Insert
    MEF2D (Mef2d Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    E287G
    Promoter
    CMV
    Available since
    Feb. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
  16. pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP

    Plasmid
    #52053
    Purpose
    Used in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.
    Depositor
    Insert
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tetR/pLtetO
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  17. p14-9VN

    Plasmid
    #50930
    Purpose
    Expresses human SRP14 and SRP9 proteins as a fusion protein in mammalian cells.
    Depositor
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Use
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  18. pCMV-Neo-Bam E2F3b

    Plasmid
    #37975
    Depositor
    Insert
    E2F3b (E2F3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2012
    Availability
    Academic Institutions and Nonprofits only
  19. pML104-NatMx3

    Plasmid
    #83477
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
    Depositor
    Insert
    NatMx3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
  20. pAAV-Ef1a-vCreDIO hChR2(H134R)-eYFP 2.0

    Plasmid
    #126081
    Purpose
    vCre-Dependent ChR2(H134R)-eYFP with the vLox sites in the reverse orientation
    Depositor
    Insert
    hChR2(H134R)-eYFP
    Use
    AAV
    Tags
    eYFP
    Expression
    Mutation
    H134R
    Promoter
    Ef1a
    Available since
    May 22, 2019
    Availability
    Academic Institutions and Nonprofits only
  21. YWHAE

    Plasmid
    #39146
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    YWHAE (YWHAE Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  22. pAP84

    Plasmid
    #86554
    Purpose
    Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile
    Depositor
    Insert
    Pveg-sLucopt
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  23. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Insert
    SA-GFP reporter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  24. pLEW-pHluorin2-PTS1

    Plasmid
    #213776
    Purpose
    Expresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal brucei
    Insert
    pHlorin2
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Appended a 2 glycine linker and a T. brucei perox…
    Promoter
    T. brucei rRNA promotor
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  25. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

    Plasmid
    #176932
    Purpose
    Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus
    Depositor
    Insert
    hGEM(1-110)_mNG_hSLBP(18-108)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK1
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  26. PTPRB

    Plasmid
    #39165
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    PTPRB (PTPRB Human)
    Use
    Tags
    GST-PreScission
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  27. pcDNA3.1-Letm1

    Plasmid
    #191962
    Purpose
    Expression of Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1).
    Depositor
    Insert
    Letm1 (LETM1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
  28. pLV V5-lamin B2

    Plasmid
    #175109
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb2
    Depositor
    Insert
    Lmnb2 (Lmnb2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
  29. pcU6.3_gRNA_c1

    Plasmid
    #221381
    Purpose
    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    G. gallus U6.3
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  30. AXL pBABE

    Plasmid
    #105936
    Purpose
    retroviral expression of AXL
    Insert
    AXL (AXL Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  31. pE-SUMO CRYAA

    Plasmid
    #80756
    Purpose
    Expression of human alpha A-crystallin in E. coli
    Depositor
    Insert
    CRYAA (CRYAA Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  32. PSIP1

    Plasmid
    #40744
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    PSIP1 (PSIP1 )
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  33. pcDNAI Neo human PECAM FL

    Plasmid
    #42902
    Depositor
    Insert
    PECAM (PECAM1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 20, 2013
    Availability
    Academic Institutions and Nonprofits only
  34. YWHAQ

    Plasmid
    #38931
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    YWHAQ (YWHAQ Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  35. pDON-AI-Npm2(N533)

    Plasmid
    #51475
    Purpose
    Express mouse phosphorylation-mimicking Npm2 (p-Npm, N533)
    Depositor
    Insert
    Nucleoplasmin 2 (Npm2 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Eleven codons (5, 6, 7, 8, 10, 82, 95, 104, 184, …
    Promoter
    Available since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
  36. pSMH1741

    Plasmid
    #160930
    Purpose
    Expression of soluble Sc octamer (H3)
    Depositor
    Inserts
    HHF1
    HTA1
    HTB1
    HHT1
    Use
    Tags
    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  37. SLC9A3R2-TwinStrepII

    Plasmid
    #83200
    Purpose
    Expression of tagged protein in mammalian cells
    Insert
    SLC9A3R2 (Slc9a3r2 Mouse)
    Use
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  38. pcDNA-Dlx6

    Plasmid
    #99480
    Purpose
    Mammalian expression of the Distal-less Homeobox 6 gene
    Depositor
    Insert
    Dlx6 (Dlx6 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  39. pcDNA5/FRT/TO/Flag-tGFP

    Plasmid
    #110375
    Purpose
    Mammalian expression of turboGFP with FLAG tag, Flp-In T-Rex system
    Insert
    turboGFP
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  40. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Use
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  41. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
  42. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  43. pDual_dsCas9_Blast_hs_TP73

    Plasmid
    #214691
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  44. CMV-AausFP1-Z1

    Plasmid
    #135048
    Purpose
    AausFP1 - bright green fluorescent protein expression from A. australis under CMV promoter in minimal backbone plasmid.
    Depositor
    Insert
    AausFP1
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Promoter
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  45. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  46. CMV-dCas9_puroR

    Plasmid
    #219825
    Purpose
    CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)
    Depositor
    Insert
    dCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    CMV
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
  47. EF1α-eGFP-Z1

    Plasmid
    #135612
    Purpose
    Enhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.
    Depositor
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-HTLV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  48. SEPX1

    Plasmid
    #39048
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    SEPX1 (MSRB1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    SeCys95-S
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only
  49. pSBbi-Pur/GDI1-splitTurboID

    Plasmid
    #198219
    Purpose
    Transposon vector (Addgene #60523) for expression of part of TurboID (up to E256) and GID1, for gibberellic acid-inducible biotin labeling
    Depositor
    Insert
    GID1
    Use
    Transposon
    Tags
    5’ part of TurboID, up to E256
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  50. pDONR221-TMPRSS4

    Plasmid
    #158446
    Purpose
    Gateway Cloning compatible entry vector for the human TMPRSS4 gene.
    Insert
    TMPRSS4 (TMPRSS4 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 3751 - 3800 of 8865 results