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We narrowed to 8,196 results for: FIE

Showing: 3041 - 3080 of 8196 results

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pBabe_puro_DEST_Flag_ARPM1

Plasmid

#45259

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

ARPM1 (ACTRT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pLVX-TRE3G-UCP1

Plasmid

#234979

Purpose

Expresses UCP1 in mouse cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

UCP1 (Ucp1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231360

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231361

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.minBG-EGFP-W3SL_BbsI(GGA)

Plasmid

#231362

Purpose

minBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231363

Purpose

Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231364

Purpose

Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231367

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231368

Purpose

Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231351

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231354

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231355

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CAG-EGFP-W3SL-BC(p1-10)

Plasmid

#231347

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3_5UTR_Myc_FLuc RBM42 deletion

Plasmid

#229503

Purpose

Myc 5' untranslated region firefly luciferase reporter with deletion of region bound by RBM42.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_Del 363

Use

Tags

Expression

Mammalian

Mutation

Deletion of bp 363-394

Promoter

SV40

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3_5UTR_Myc_FLuc Compensatory Mutant

Plasmid

#229504

Purpose

Myc 5' untranslated region firefly luciferase reporter with compensatory mutations in the region bound by RBM42

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_CompMutant

Use

Tags

Expression

Mammalian

Mutation

Mutations in bp 363-394

Promoter

SV40

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-eA3A-BE5-(NG)-PX458-EGFP

Plasmid

#229687

Purpose

CRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.

Depositor

Deborah Marsh

Article

Dickson et al Hum Mol Genet. 2023 Jun 27:ddad105. doi:

Type

Empty backbone

Use

CRISPR

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MKI-MUT-P2A-GFP

Plasmid

#229406

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-MUT-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

interaction mutant L109G; L143G (Control mutant)

Promoter

EFS

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MKI-MUT-P2A-GFP-DOX-N

Plasmid

#229408

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-MUT-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

interaction mutant L109G; L143G (Control mutant)

Promoter

TRE3G

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MAP4K1

Plasmid

#229396

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MAP4K1 (MAP4K1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-CDC25C

Plasmid

#229410

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

CDC25C (CDC25C Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A empty

Plasmid

#213164

Purpose

Empty vector (no sgRNA) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa and U6

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A+sgGFP

Plasmid

#213165

Purpose

Vector with sgGFP for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgGFP

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa and U6 and PGK

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A+sgAAVS1_145

Plasmid

#213166

Purpose

Vector with sgAAVS1 for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgAAVS1_145

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa and U6

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SAM-DNMT3A+sgAAVS1_118

Plasmid

#213167

Purpose

Vector with sgAAVS1 for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgAAVS1_118

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa and U6

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SAM-DNMT3A-inactiveEmpty

Plasmid

#213168

Purpose

Empty Vector (no sgRNA) used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa and U6

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGI_Myr-YFP-MiniTurboID

Plasmid

#222432

Purpose

Potential plasma membrane control.

Depositor

Dominique Bergmann

Article

Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

Insert

MiniTurboID (BirA mutant)

Use

Golden gate / green gate compatible cloning vector

Tags

Linker, Myr, and YFP

Expression

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS

Plasmid

#194753

Purpose

Lentiviral expression of BioID2-HA-13 x Linker- RBP(V) on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS

Plasmid

#194752

Purpose

Lentiviral expression of RBP(V)-13 x Linker-BioID2-HA on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS1983 Lenti-dead_GFP_ABE reporter-BlastR

Plasmid

#211820

Purpose

Lentiviral vector expressing a dead GFP adenine base editing reporter

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

dead GFP reporter

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter and SV40 promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS-HZ08-pAAVsc.U6-tracrRNA

Plasmid

#211816

Purpose

AAV vector expressing tracrRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

U6-tracrRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMtnA FLAG-IntS7 puro

Plasmid

#208405

Purpose

Expresses FLAG-tagged Drosophila IntS7 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

pMT FLAG MCS puro (defl Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

MtnA

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMtnA FLAG-IntS13 puro

Plasmid

#208406

Purpose

Expresses FLAG-tagged Drosophila IntS13 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS13 (asun Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

MtnA

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMtnA FLAG-IntS14 puro

Plasmid

#208407

Purpose

Expresses FLAG-tagged Drosophila IntS14 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS14 (IntS14 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

MtnA

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMtnA FLAG-IntS6 puro

Plasmid

#195076

Purpose

Expresses FLAG-tagged Drosophila IntS6 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

MtnA

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMtnA FLAG-IntS12 puro

Plasmid

#195077

Purpose

Expresses FLAG-tagged Drosophila IntS12 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator 12 (IntS12 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

MtnA

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUb FLAG-IntS6 AA 1-400

Plasmid

#195071

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 1-400

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ubi-p63e

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUb FLAG-IntS6 AA 1-600

Plasmid

#195072

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 1-600

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ubi-p63e

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUb FLAG-IntS6 AA 101-1284

Plasmid

#195073

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 101-1284

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ubi-p63e

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUb FLAG-IntS6 AA 1197-1284

Plasmid

#195074

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 1197-1284

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ubi-p63e

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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