We narrowed to 120,127 results for: CIL

1. pJMP1

   Plasmid

   #79873

   Purpose

   Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

   Depositor

   Carol Gross, Jason Peters, Stanley Qi

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   xylA

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

   Plasmid

   #138148

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NES

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CoV2-M-IRES-E

   Plasmid

   #177938

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbA5a-sufCDSUB

   Plasmid

   #195055

   Purpose

   Expresses SUF operon from B. subtilis from IPTG-inducible promoter

   Depositor

   Greg Bokinsky

   Article

   D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

   Insert

   sufCDSUB
   

   Expression

   Bacterial

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOP01-UTC-7.0

   Plasmid

   #216625

   Purpose

   NanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test construct

   Depositor

   Henrike Niederholtmeyer

   Article

   Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

   Insert

   T7p-5'g10-NanoLuc-3'TMV
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCWori cytochrome P450-BM3h 9D7

   Plasmid

   #61308

   Purpose

   Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

   Depositor

   Alan Jasanoff

   Article

   Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

   Insert

   Cytochrome P450-BM3 heme domain 9D7
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T268A, I263A, T438V, A328G

   Promoter

   Tac

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF5B-FRT-GFP-αTAT1[D157N]

   Plasmid

   #27100

   Depositor

   Maxence Nachury

   Article

   Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

   Insert

   αTubulin K40 acetyltransferase (ATAT1 Human)
   

   Tags

   GFP and S-tag

   Expression

   Mammalian

   Mutation

   D157N

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Luc-PS9

   Plasmid

   #177942

   Purpose

   Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   Luc2 (ORF1ab )
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Luc-T20

    Plasmid

    #177941

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2 (ORF1ab )
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF5-FRT-TagRFP-T-IFT88

    Plasmid

    #61684

    Purpose

    Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    IFT88 (Ift88 Mouse)
    

    Tags

    TagRFP.T

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IPU-GFP-MYH9

    Plasmid

    #168273

    Purpose

    Expresses MYH9 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    MYH9 (MYH9 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBSII-PGK-FLPo-IRES-Puro

    Plasmid

    #205989

    Purpose

    Expresses Flp recombinase and puromycin resistance

    Depositor

    Sharon Torigoe

    Article

    Waite et al PLoS One. 2024 Sep 30;19(9):e0311120. do

    Inserts

    PGK promoter
    FLPo (flp )
    IRES-PuroR cassette
    

    Expression

    Mammalian

    Promoter

    PGK promoter

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5FRT/TO-p47-Strep-HA

    Plasmid

    #113475

    Purpose

    Expression of human p47 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    p47 (NSFL1C Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-TPH2-Cre

    Plasmid

    #189616

    Purpose

    Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Cre Recombinase
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Tph2

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSP6-RNAP

    Plasmid

    #48143

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase SP6
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    V314A (numbering based on NCBI Sequence ID: NP_85…

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiRNAGuide_003

    Plasmid

    #192505

    Purpose

    For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. TALED_Left-mouse-ATP6-1397N.

    Plasmid

    #212951

    Purpose

    Expresses TALED_Left-mouse-ATP6-1397N in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_Left-mouse-ATP6-1397N (ATP6 Synthetic)
    

    Expression

    Mammalian

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

    Plasmid

    #176280

    Purpose

    Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mNaChBac-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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