We narrowed to 18,831 results for: Tec

1. pBT120a17

   Plasmid

   #138510

   Purpose

   P1

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   gIII-xLuxAB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQE30-His-IF3

   Plasmid

   #124125

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Initiation factor 3 (infC Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG1188 mCherry-cagedFIREtag

   Plasmid

   #205177

   Purpose

   Expresses mCherry-cagedFIREtag in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   mCherry-FIREtag-DEVDG-MoA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQE30-His-RF3

   Plasmid

   #124131

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 3 (prfC Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQE60-EFTs-His

   Plasmid

   #124128

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Elongation factor Ts (tsf Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET15b-His-RF2

   Plasmid

   #124130

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 2 (prfB Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQE60-RRF-His

   Plasmid

   #124132

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Ribosome recycling factor (frr Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTXB1-nbMmIgG2a-Tn5

   Plasmid

   #184288

   Purpose

   Anti-mouse IgG2a Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

   Depositor

   New York Genome Center, Ivan Raimondi

   Article

   Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

   Insert

   nbMmIgG2a-Tn5
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDAC446

   Plasmid

   #195239

   Purpose

   Mammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT2ST

    Plasmid

    #59384

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-dfrA
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYMA1_SidK_mCH

    Plasmid

    #184906

    Purpose

    Legionella pneumophila SidK (1-278) - mCherry

    Depositor

    John Rubinstein

    Article

    Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

    Insert

    SidK (1-278) (sidK Legionella pneumophila)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pS24·Pm-GFP

    Plasmid

    #122594

    Purpose

    Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    Pm promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC19/N-Vc

    Plasmid

    #183157

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPAGFP-C1

    Plasmid

    #11910

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

    Has Service

    Cloning Grade DNA

    Insert

    PAGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    These plasmids are essentially the same as the Cl…

    Promoter

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pColE1-ISceI

    Plasmid

    #179578

    Purpose

    Expresses I-SceI from an arabinose promoter

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    I-SceI (I-SceI Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-dGBP1-TagBFP

    Plasmid

    #80086

    Purpose

    GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Insert

    dGBP1-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EasyFusion T2A-H2B-TagBFP

    Plasmid

    #113086

    Purpose

    General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-TagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBT128c

    Plasmid

    #138512

    Purpose

    P2

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    inteinC-dSpCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTlpA_mCherry

    Plasmid

    #229146

    Purpose

    Promoter strength evaluation of PtlpA

    Depositor

    Shrikrishnan Sankaran

    Article

    Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No

    Promoter

    PtlpA

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JD553

    Plasmid

    #160392

    Purpose

    Wheat GRF4-GIF1 chimera in pDONR-zeo

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    wheat GRF4-GIF1 chimera
    

    Use

    Entry clon

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pX330A_dCas9-1x4

    Plasmid

    #63598

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 4xNLS-pMJ915v2

    Plasmid

    #88917

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ABE-RA3.0

    Plasmid

    #208301

    Purpose

    Expresses ABE-RA3.0 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA3.0-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBB572

    Plasmid

    #27397

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    ibpA, ibpB (ibpB, ibpA E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC19/Vc-C

    Plasmid

    #183158

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET19b-rDAD2 cPGFP(G164)

    Plasmid

    #165994

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rDAD2 cPGFP(G164)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTol2-hspDRv7_scGstlt

    Plasmid

    #108870

    Purpose

    scGESTALT V7 lineage barcode for zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Insert

    scGESTALT lineage tracing target V7
    

    Use

    Tol2 integration into zebrafish genome

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJK_kanR_proJK1_PAD

    Plasmid

    #60630

    Purpose

    Constitutively expresses PAD (phenolic acid decarboxylase)

    Depositor

    Timothy Whitehead

    Article

    Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

    Insert

    Phenolic acid decarboxylase padC (padC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K146Y

    Promoter

    proJK1

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRAM24

    Plasmid

    #234313

    Purpose

    pBBR1 origin cat-RNA U1376

    Depositor

    James Chappell

    Article

    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

    Insert

    U1367 Targeting RAM, Barcode 1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P. CymRC

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBI-MCS-EYFP

    Plasmid

    #58855

    Purpose

    The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCRISPomyces-SaCas9

    Plasmid

    #129553

    Purpose

    Contains codon-optimized SaCas9 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    SaCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HA-RNF4/pRK5

    Plasmid

    #59743

    Purpose

    Expresses RNF4 in mammalian cells

    Depositor

    Xiaolu Yang

    Article

    Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBB535

    Plasmid

    #27392

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    dnaK, dnaJ (dnaJ, dnaK E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC19/N-eCFP

    Plasmid

    #183155

    Purpose

    Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFP

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1 BoMo N-1F

    Plasmid

    #203761

    Purpose

    For expression of N-flag tagged BoMo R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Reporter-1

    Plasmid

    #180218

    Purpose

    Surrogate reporter plasmid for the assay of targeted RNA editing

    Depositor

    Wensheng Wei

    Article

    Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

    Insert

    Surrogate reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p15A_SpyCas9_CmR

    Plasmid

    #119162

    Purpose

    Expresses S. pyogenes Cas9

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    Cas9 from S. pyogenes (NEWENTRY )
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBT376

    Plasmid

    #125617

    Purpose

    Expresses BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    BE4max-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDAC569

    Plasmid

    #195241

    Purpose

    Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pYL322d1/N-Vn

    Plasmid

    #183153

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge