We narrowed to 256 results for: Map2

1. pWZLblasti-HA-MEK5

   Plasmid

   #53167

   Purpose

   Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK5 (MAP2K5 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

   Plasmid

   #87776

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK4 (MAP2K4 Human)
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF0702

   Plasmid

   #144361

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NCOR2 (NCOR2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs2-MEK1

   Plasmid

   #86141

   Purpose

   Retroviral vector expressing MEK1-IRES-Bsd-p2A-RFP

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-Mkk7-JNK2 fusion-pcw107

   Plasmid

   #64618

   Purpose

   Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   Map2k7 linker MAPK9
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   mouse-human fusion

   Promoter

   PGK

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

6. TFORF0703

   Plasmid

   #144072

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NCOR2 (NCOR2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF0704

   Plasmid

   #143726

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NCOR2 (NCOR2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NES-EGFP-cPHx2

   Plasmid

   #116854

   Purpose

   PI(3,4)P2 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Use

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   amino acids 169-329 (tandem dimer)

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MBP-MEK1 S222TAG

   Plasmid

   #68302

   Purpose

   N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   MBP-MEK1 S222TAG (MAP2K1 Synthetic, Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEBG-MKK4-S255D/T259E (GST)

    Plasmid

    #21566

    Depositor

    Leonard Zon

    Article

    Sanchez et al Nature. . 372(6508):794-8.

    Insert

    mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Serine 255 to Aspartic Acid, Threonine 259 to Gl…

    Promoter

    Available since

    July 17, 2009

    Availability

    Academic Institutions and Nonprofits only

11. MBP-MEK1 S218TAG

    Plasmid

    #68301

    Purpose

    N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    MBP-MEK1 S218TAG (MAP2K1 Synthetic, Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEBG-MKK4-S255E/T259D (GST)

    Plasmid

    #21565

    Depositor

    Leonard Zon

    Article

    Sanchez et al Nature. . 372(6508):794-8.

    Insert

    mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Serine 255 to Glutamic Acid, Threonine 259 to As…

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pHH0103_ASAP2-1/1

    Plasmid

    #91504

    Purpose

    Protein expression and purification of human SH3 domain construct ASAP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ASAP2-1/1 (ASAP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLIK 3XFLAG-MKK7-EE-NLS hygro

    Plasmid

    #87780

    Purpose

    Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK7 (Map2k7 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pGEX6P1-HA-MEK5 CBM-E

    Plasmid

    #53168

    Purpose

    Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    L280A/H281A/M323A/Q332A

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pWZLblasti-HA-MEK5 CBM-E

    Plasmid

    #53169

    Purpose

    Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L280A/H281A/M323A/Q332A

    Promoter

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

18. NES-EGFP-PH-ARNO2G-I303Ex2

    Plasmid

    #116868

    Purpose

    PIP3 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    CYTH2 (CYTH2 Human)
    

    Use

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    amind acids 252-399, isoleucine 303 changed to gl…

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-myc-ERK2-MEK1_fusion

    Plasmid

    #39194

    Depositor

    Melanie Cobb

    Article

    Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

    Insert

    ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pWZLblasti-HA-MEK1-MEK5DD

    Plasmid

    #53177

    Purpose

    Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    MEK1 (1-201) MEK5(498–1344 with S311D/T315D)

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pEN_TT 3xHA-MKK6-EE

    Plasmid

    #47544

    Purpose

    Gateway entry vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

    Insert

    3xHA-MKK6-EE (MAP2K6 Human)
    

    Use

    Entry vector

    Tags

    3XHA

    Expression

    Mutation

    S207E, T211E

    Promoter

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR223-MEKDD

    Plasmid

    #31202

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    MAPKK1 (MAP2K1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    S218D S222D

    Promoter

    Available since

    June 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pINDUCER22-MKK6E-Flag

    Plasmid

    #99259

    Purpose

    Lentiviral vector encoding a doxycycline-inducible Flag-tagged constitutively active MKK6.

    Depositor

    Peter Vincent, Alejandro Adam

    Article

    Adam et al PLoS One. 2016 Sep 7;11(9):e0161975. doi

    Insert

    MKK6E (MAP2K6 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S207E T211E

    Promoter

    TRE2

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-myc-ERK2-L4A-MEK1_fusion

    Plasmid

    #39197

    Depositor

    Melanie Cobb

    Article

    Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

    Insert

    ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L4A = L33A, L37A, L40A, L42A

    Promoter

    CMV

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSLIK 3xHA-MKK6-EE neo

    Plasmid

    #47546

    Purpose

    Lentiviral expression vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

    Insert

    MKK6-EE (MAP2K6 Human)
    

    Use

    Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    S207E, T211E

    Promoter

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LAMTOR3 gRNA (BRDN0001147669)

    Plasmid

    #77136

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LAMTOR3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LAMTOR3 (LAMTOR3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LAMTOR3 gRNA (BRDN0001145475)

    Plasmid

    #77134

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LAMTOR3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LAMTOR3 (LAMTOR3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LAMTOR3 gRNA (BRDN0001145977)

    Plasmid

    #77135

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LAMTOR3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LAMTOR3 (LAMTOR3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LAMTOR3 gRNA (BRDN0001147910)

    Plasmid

    #77137

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LAMTOR3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LAMTOR3 (LAMTOR3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge