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Showing: 281 - 320 of 348 results

  1. pCAG-VHC

    Plasmid
    #112618
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112618-plasmid-map-sequence-id-222032

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  2. pCAG-tdTHC

    Plasmid
    #112621
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-tdTomato (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Cre/Lox
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112621-plasmid-map-sequence-id-222036

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  3. pCAG-mTHC

    Plasmid
    #112620
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112620-plasmid-map-sequence-id-226488

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  4. ptdTHC

    Plasmid
    #112617
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-tdTomato (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112617-plasmid-map-sequence-id-224553

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  5. pVHC

    Plasmid
    #112614
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112614-plasmid-map-sequence-id-224545

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  6. pCAG-EHC

    Plasmid
    #112619
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112619-plasmid-map-sequence-id-226490

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  7. pEHC

    Plasmid
    #112615
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Emerald
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112615-plasmid-map-sequence-id-226350

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  8. pmTHC

    Plasmid
    #112616
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112616-plasmid-map-sequence-id-224548

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  9. MTK0_046

    Plasmid
    #123976
    Purpose
    Encodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemh
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    hThal5.10-2_hg18 CAS9 Destination - HygroR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123976-plasmid-map-sequence-id-251798

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  11. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

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  12. pCol1a1-frt (FVB)

    Plasmid
    #63575
    Purpose
    Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Col1A-frt-hygro-pA
    Use
    Mouse Targeting; Frt/flpe
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    None
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63575-plasmid-map-sequence-id-105719

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  13. pAG32-L-IFP-F[2] (clon 3)

    Plasmid
    #52900
    Purpose
    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    L-IFP-F[2]
    Use
    Vector for homologous recombination
    Tags
    Expression
    Mutation
    Promoter
    TEF promoter
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. CMV+ episome

    Plasmid
    #169742
    Purpose
    CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169742-plasmid-map-sequence-id-334220

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  16. PGK+ episome

    Plasmid
    #170041
    Purpose
    PGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    170041-plasmid-map-sequence-id-334558

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  17. pYUBcLIC-GFP

    Plasmid
    #183509
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    183509-plasmid-map-sequence-id-362497

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  19. pGoldenGreenGate-M (pGGG-M)

    Plasmid
    #165422
    Purpose
    A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology; Level 2 golden gate (moclo) du…
    Tags
    Expression
    Mutation
    Promoter
    Contain GG cassette and MCS with LacZ for blue/wh…
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165422-plasmid-map-sequence-id-331272

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  21. PB-SAM-Dest

    Plasmid
    #102563
    Purpose
    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    N/A
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102563-plasmid-map-sequence-id-193893

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  24. pMDC32B-AtMIR390a-B/c

    Plasmid
    #51776
    Purpose
    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtMIR390a-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51776-plasmid-map-sequence-id-80341

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  25. pMDC32B-AtTAS1c-B/c

    Plasmid
    #51773
    Purpose
    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtTAS1c-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51773-plasmid-map-sequence-id-80112

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  30. Equalizer-L episome

    Plasmid
    #169738
    Purpose
    Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169738-plasmid-map-sequence-id-334158

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  32. pMDC32B-B/c

    Plasmid
    #227963
    Purpose
    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka
    Insert
    B/c
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227963-plasmid-map-sequence-id-454828

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  33. pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a

    Plasmid
    #213401
    Purpose
    Plant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursor
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:
    Insert
    Arabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    213401-plasmid-map-sequence-id-422351

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  34. pCas3-APOBEC1

    Plasmid
    #229536
    Purpose
    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229536-plasmid-map-sequence-id-458911

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  35. pCas3-CDA1

    Plasmid
    #229534
    Purpose
    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229534-plasmid-map-sequence-id-458891

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  36. lentiGuide-chr20-49345720-gRNA1

    Plasmid
    #232623
    Purpose
    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.
    Depositor
    Claes Wadelius
    Article
    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1
    Insert
    S. pyogenes sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    April 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232623-plasmid-map-sequence-id-465706

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  37. lentiGuide-chr20-49345720-gRNA2

    Plasmid
    #232624
    Purpose
    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.
    Depositor
    Claes Wadelius
    Article
    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1
    Insert
    S. pyogenes sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    April 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232624-plasmid-map-sequence-id-465707

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  38. lentiGuide-chr20-49345720-gRNA3

    Plasmid
    #232625
    Purpose
    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.
    Depositor
    Claes Wadelius
    Article
    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1
    Insert
    S. pyogenes sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    April 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232625-plasmid-map-sequence-id-465708

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Showing: 281 - 320 of 348 results