We narrowed to 18,831 results for: Tec

1. pAG1345 mCherry-FIREtag-DEVDG-MoA

   Plasmid

   #205176

   Purpose

   Expresses mCherry-FIREtag-DEVDG-MoA in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   mCherry-cagedFIREtag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAC575

   Plasmid

   #127976

   Purpose

   AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   ble (bleomycin resistance marker)
   

   Use

   Fungal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans trpC promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DL017:CMV::HaloTag-iPAK4

   Plasmid

   #177882

   Purpose

   HT-iPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS426 Gal-RNQ1-YFP

   Plasmid

   #18686

   Depositor

   Susan Lindquist

   Article

   Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

   Insert

   Rnq1 (RNQ1 Budding Yeast)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

5. Tet-O-FUW-Sox10

   Plasmid

   #45843

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

   Insert

   Sox10 (Sox10 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCpf1-sp

   Plasmid

   #122186

   Purpose

   Bacterial genome editing plasmid with spectinomycin resistance marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-M13-TEV-C-P2A-TdTomato

   Plasmid

   #92389

   Purpose

   expresses Cal-light M13-TEV-C component and TdTomato in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   M13-TEV-C-P2A-TdTomato
   

   Use

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGNW4

   Plasmid

   #122088

   Purpose

   suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfB4586

   Plasmid

   #106124

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTW211b5

    Plasmid

    #136927

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9(N)-NpuN
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    see manuscript

    Promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG1242 NLS-mCherry-FIREtag

    Plasmid

    #205170

    Purpose

    Expresses NLS-mCherry-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    NLS-mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKD238-1a

    Plasmid

    #90958

    Purpose

    Expresses S. baltica TtrS constitutively

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Insert

    Tetrathionate sensor kinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_J23109

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMR-A-3

    Plasmid

    #138517

    Purpose

    P3a

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCfB3405

    Plasmid

    #106166

    Purpose

    EasyCloneYALI system-based yeast episomal vector carrying a nourseothricin-resistance marker for Yarrowia lipolytica, can be used for gRNA expression, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDYU1B/mRuby3–α-tubulin

    Plasmid

    #154302

    Purpose

    Expresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideum

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Insert

    alpha-tubuin
    

    Use

    Dictyostelium expression

    Tags

    mRuby3

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUCIDT-AMP-Bv16SrRNA

    Plasmid

    #218518

    Purpose

    pUCIDT-AMP containing 16S rRNA gene of B. vulgatus for absolute concentration quantification

    Depositor

    Ami Bhatt

    Article

    Maghini et al Nat Biotechnol. 2024 Feb;42(2):328-338.

    Insert

    16S rRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PlayBack-CMV-EcoR1

    Plasmid

    #203305

    Purpose

    For expression of eukaryotic genes of interest with CMV promoter with EcoRI as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDM_T7_HisLacZomega

    Plasmid

    #118815

    Purpose

    T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    lacZ_
    

    Use

    Tags

    histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB UbC Arrestin-uTEV2-V5; UAS-mCherry

    Plasmid

    #173123

    Purpose

    Expresses uTEV2 and reporter receiver construct in DIPG

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    Arrestin-uTEV2-V5; UAS-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMR-A-5

    Plasmid

    #138519

    Purpose

    P3c

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC19/mChe

    Plasmid

    #183161

    Purpose

    Construct for transient expression of cell nucleus-, cytoplasm-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 4xNLS-pMJ915v2-sfGFP

    Plasmid

    #88921

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCfB2197

    Plasmid

    #67551

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MSGV Hu Acceptor

    Plasmid

    #64269

    Purpose

    Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    none

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. IVT-MCP-Phi29

    Plasmid

    #207456

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    MCP-Phi29
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFPL-Ch

    Plasmid

    #61647

    Purpose

    Gateway vector for generating cyan fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced cyan fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p201B Cas9

    Plasmid

    #59177

    Purpose

    Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    BAR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMT445

    Plasmid

    #139386

    Purpose

    aTC inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    aTC inducible Nanoluc expression
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    aTC inducible promoter

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUC19/mChe-C

    Plasmid

    #183160

    Purpose

    Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMT444

    Plasmid

    #139385

    Purpose

    IPTG inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    IPTG inducible Nanoluc expression,
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    IPTG inducible promoter

    Available Since

    July 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUC19/eCFP-C

    Plasmid

    #183156

    Purpose

    Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFP

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1-IL2sp-6His-Asprosin plasmid

    Plasmid

    #190683

    Purpose

    Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

    Depositor

    Atul Chopra

    Article

    Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

    Insert

    IL2-6xHis-Asprosin
    

    Use

    Tags

    IL2 signal peptide, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGEX-4T-1-p19-T7

    Plasmid

    #46306

    Depositor

    Judy Lieberman

    Article

    Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

    Inserts

    p19
    DNA-1 site
    linker
    DNA-2 site
    

    Use

    Pro-sirna

    Tags

    6XHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pBT138b-proB

    Plasmid

    #138515

    Purpose

    P3f

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ttAtCas12a+int

    Plasmid

    #182384

    Purpose

    Gives very high editing efficiency in barley. Also works in Brassica oleracea

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al bioRxiv 2022.04.28.489853

    Insert

    LbCas12a coding sequence with D156R and Arabidopsis introns
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R + 8 Arabidopsis introns added

    Promoter

    None

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Ca-FLARE (protease)

    Plasmid

    #92214

    Purpose

    protease component of FLARE

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    CaM-V5-TEVp(Δ220-242)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available Since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLentiCRISPR-v2 sgATG7

    Plasmid

    #211534

    Purpose

    Deletes ATG7

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgATG7
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. YEp195-CUP1-His-Ubi

    Plasmid

    #99540

    Purpose

    Yeast episomal vector for expression of His-tagged ubiquitin; URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    UBI3(1-76) (RPL40A Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p8.9 NdeltaSB D185K/D186L

    Plasmid

    #132931

    Purpose

    p8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase sequence

    Depositor

    Jeremy Luban

    Article

    De Iaco et al Retrovirology. 2011 Dec 6;8:98. doi: 10.

    Insert

    gag and pol D185K/D186L
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D185K/D186L

    Promoter

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUC-DEST-hPROX1-tdTomato-PuroTK

    Plasmid

    #141027

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge