12,935 results

Showing: 2401 - 2430 of 12935 results

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pcDNA3.1(+) Drp1 V2

Plasmid

#44599

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 variant 2 (Dnm1l Mouse)

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pTA-Luc SIRT1 promoter (-202)

Plasmid

#10971

Depositor

Toren Finkel

Article

Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

Insert

SIRT1 promoter (Sirt1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (W131G)

Plasmid

#169724

Purpose

Mammalian Expression of mouse SPOP W131G

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

W131G

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

Plasmid

#161580

Purpose

Express Kir2.1 in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

Plasmid

#184395

Purpose

Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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mP58.FL1-pCDNA3

Plasmid

#21883

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mSTING_scrambled_gRNA_2

Plasmid

#196628

Purpose

Scrambled control 2 for STING knock-out in murine cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING scrambled gRNA 2

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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HA-Vim

Plasmid

#46315

Depositor

Xiangli Yang

Article

Lian et al J Biol Chem. 2009 Oct 30;284(44):30518-2

Insert

Vimentin (Vim Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-SUN1 GFP

Plasmid

#187682

Purpose

In vivo visualization of SUN1

Depositor

Mary Beckerle

Insert

SUN1 (Sun1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-Wnt3a

Plasmid

#114287

Purpose

TET-inducible expression of Wnt3a

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

Wnt3a (Wnt3a Mouse)

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pmNFL

Plasmid

#83127

Purpose

Mouse neurofilament protein L cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFL (Nefl Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-ADGRL3-Flag

Plasmid

#201218

Purpose

pCDNA3.1 with kozak (GCC), ADGRL3 and C-terminal Flag

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ran-Q69L-mRFP1-polyA

Plasmid

#104560

Purpose

Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Tags

mRFP1

Expression

Mammalian

Mutation

Q69L

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK K454R

Plasmid

#50501

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

K454R

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-GRAB_NTS1.0

Plasmid

#180006

Purpose

Expresses the neurotensin sensor GRAB_NTS1.0 in neurons

Depositor

Yulong Li

Insert

neurotensin sensor GRAB_NTS1.0

Use

AAV

Promoter

hSyn

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGL6

Plasmid

#48746

Purpose

Expression of luciferase driven by mPer2 promoter region (bases -1128 to +2129 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

mPer2 promoter/enhancer region (-1128 to +2129)

Use

Luciferase

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-FlagMLL2-pL-ENL(5613)

Plasmid

#20874

Depositor

Robert Slany

Article

Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

Insert

MLL2 (Kmt2d, MLLT1 Human, Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only
Opto-ACTU−

Plasmid

#198869

Purpose

Optogenetic recruitment construct to increase inner membrane surface charge

Depositor

Peter Devreotes

Article

Banerjee et al Nat Cell Biol. 2022 Oct;24(10):1499-1515

Insert

Rad17 polyanionic tail (-14)

Use

Synthetic Biology

Tags

CRY2PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGST-DHFR Mm

Plasmid

#83073

Purpose

Expresses the full length DHFR Mm in E.coli

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

2x DHFR (Dhfr Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP680-P2A-GFP

Plasmid

#197164

Purpose

Expresses the protein of miRFP680-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680-P2A-GFP

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-KO

Plasmid

#20331

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Klf4-F2A-Oct4

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCEFL3xHAmGli2

Plasmid

#37671

Depositor

Philip Beachy

Article

Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

Gli2 (Gli2 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

EF1a

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-Trim46

Plasmid

#176402

Purpose

TRIM46 tagged with GFP

Depositor

Casper Hoogenraad, Thomas Schwarz

Article

van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

Insert

trim46 (Trim46 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-CD44v4

Plasmid

#107969

Purpose

expression of CD44 variant

Depositor

Georg Weber

Insert

mCD44v4 (Cd44 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

splice variant; see Depositor Comments below

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Unc5b.z-AP-His

Plasmid

#72053

Purpose

Expresses the extracellular region of the Unc5B, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5b.z (Unc5b Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE Cplx1-GFP KI

Plasmid

#131475

Purpose

Endogenous tagging of Complexin1: C-terminal (amino acid position: L128)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cplx1, Cplx1 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-KIF1A*-strep

Plasmid

#120165

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 1A (Kif1a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF1A: 1-405 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCx43-7-iRFP670-N1

Plasmid

#197222

Purpose

Expresses the fusion protein of Cx43-7-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-iRFP670

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP682

Plasmid

#197204

Purpose

Expresses the protein of iRFP682 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP682

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv2.1 K+ channel scFv [K39/25]

Plasmid

#182067

Purpose

Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K39/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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12,935 results