We narrowed to 5,559 results for: Chia

1. pCis GFPuv-SS Himar1 A7

   Plasmid

   #84686

   Purpose

   Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

   Depositor

   Ulrike Munderloh

   Article

   Munderloh et al Intracellular Pathogens II: Rickettsiale

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBlueKan+cysMPro

   Plasmid

   #187879

   Purpose

   Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

   Depositor

   Gaylen Uhlich

   Article

   Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CysM

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PT7lacO-His6-IF2

   Plasmid

   #224367

   Purpose

   Express IF2 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   IF2 (infB E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PT7lacO-RF3-His6

   Plasmid

   #224373

   Purpose

   Express RF3 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   RF3 (prfB E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PT7lacO-His6-PheRSa-PheRSb

   Plasmid

   #224364

   Purpose

   Express PheRS protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   PheRS (pheS E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PT7lacO-His6-AsnRS

   Plasmid

   #224363

   Purpose

   Express AsnRS protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   AsnRS (asnS E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PT7lacO-RRF-His6

   Plasmid

   #224374

   Purpose

   Express RRF protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   RRF (frr E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PT7lacO-His6-IF3

   Plasmid

   #224368

   Purpose

   Express IF3 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   IF3 (infC E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PT7lacO-His6-IF1

   Plasmid

   #224366

   Purpose

   Express IF1 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   IF1 (infA E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-2(KW)2-mCherry

    Plasmid

    #112738

    Purpose

    Express 2(KW)2-mCherry in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

    Insert

    2(KW)2-mCherry
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAC-BETAipi-ispA/dxs

    Plasmid

    #178302

    Purpose

    Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Inserts

    ispA
    dxs
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJLG039

    Plasmid

    #192982

    Purpose

    BHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoter

    Depositor

    Clay Fuqua

    Article

    Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

    Insert

    T7 polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. G555

    Plasmid

    #216483

    Purpose

    Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

    Depositor

    Christophe Danelon

    Insert

    plsB, plsC, cdsA, pssA
    

    Use

    Cell-free expression

    Tags

    Expression

    Mutation

    Promoter

    T7 for each gene

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-E. coli Initiation Factor 3 (IF3)

    Plasmid

    #31340

    Depositor

    Linda Spremulli

    Article

    Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

    Insert

    E. coli Initiation Factor 3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

15. PAS528 (TJF059)

    Plasmid

    #65602

    Purpose

    pETDuet-1 Containing FadD-His

    Depositor

    Pamela Silver

    Article

    Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

    Insert

    FadD
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lacO

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

16. RP4-7

    Plasmid

    #79811

    Purpose

    Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Chloramphenicol acetyltransferase
    

    Use

    Helper plasmid for mobilization

    Tags

    Expression

    Mutation

    Promoter

    Own promoter

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pJMP8045

    Plasmid

    #220900

    Purpose

    Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    tnsABCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMA2641

    Plasmid

    #25435

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

    Insert

    reverse tetracycline-regulated transactivator advanced
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKSJ331

    Plasmid

    #103063

    Purpose

    Expresses colicin E1 and E1 immunity protein without E1 lysis (kil) gene

    Depositor

    Alan Finkelstein, Karen Jakes

    Article

    Kienker et al J Gen Physiol. 2008 Dec;132(6):693-707.

    Insert

    Colicin E1 and E1 immunity protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    colicin promotor from ColE1

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28b-6His-AcpS

    Plasmid

    #145376

    Purpose

    Expression of E. coli AcpS

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    AcpS (acpP E. coli)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pDEST17-UnaG

    Plasmid

    #203485

    Purpose

    For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMA3735

    Plasmid

    #184848

    Purpose

    gRNA cloning using blue-white screening for recombinants

    Depositor

    Mikhail Alexeyev

    Article

    Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

    Insert

    LacZalpha
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    alpha-fragment of LacZ

    Promoter

    U6

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJB153

    Plasmid

    #92328

    Purpose

    Express mEos3.2 tagged ZapA in E coli

    Depositor

    Jie Xiao

    Article

    Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

    Insert

    ZapA
    

    Use

    Tags

    mEos3.2

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSLS.436

    Plasmid

    #184953

    Purpose

    Express -Eco1 RT and ncRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: ncRNA(wt) and RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. OmpT

    Plasmid

    #213137

    Purpose

    OmpT_native membrane expression

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    OmpT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CAG.mCherry-eDHFR

    Plasmid

    #176201

    Purpose

    Negative control of targeted optogenetic manipulation using TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    mCherry-eDHFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSWAP_Entry_H2_beta_CM

    Plasmid

    #184870

    Purpose

    pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pET28a-clbC

    Plasmid

    #114145

    Purpose

    Expresses ClbC-nHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbC
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-clbH-A1

    Plasmid

    #114149

    Purpose

    Expresses ClbH-A1

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbH-A1
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Contains only the A1 domain of ClbH

    Promoter

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSWAP_Entry_HAIB

    Plasmid

    #184869

    Purpose

    For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pSWAP_Entry_H2_beta_GM

    Plasmid

    #184871

    Purpose

    pSwap plasmid part with H2_beta homology arms and gentamycin cassette

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pICXNHG3

    Plasmid

    #49021

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. vgCam-pRSETb

    Plasmid

    #193362

    Purpose

    A FRET-based calcium probe using Sumire

    Depositor

    Takeharu Nagai

    Article

    Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

    Insert

    vgCam
    

    Use

    E.coli expression

    Tags

    His X6 tag and Strep tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKS104

    Plasmid

    #24632

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    fadD (M3351), atfA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJexpress414_OmpA171_WT

    Plasmid

    #62646

    Purpose

    Wild-type OmpA (N-terminal domain) with an HA tag in the 4th external loop

    Depositor

    Timothy Whitehead

    Article

    Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

    Insert

    OmpA
    

    Use

    Synthetic Biology

    Tags

    6His and FlAsH

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pICXC3GH

    Plasmid

    #49023

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGR-BBa_B0010

    Plasmid

    #46003

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Insert

    BBa_B0010 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJL005

    Plasmid

    #92327

    Purpose

    Express FtsZ tagged with mEos3.2 in E coli

    Depositor

    Jie Xiao

    Article

    Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

    Insert

    FtsZ
    

    Use

    Tags

    mEos3.2

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFF145

    Plasmid

    #61446

    Purpose

    aTc-inducible Beta recombinase

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    Beta recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pTetO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDB328

    Plasmid

    #113672

    Purpose

    expresses Ulp1_R4 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R4
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G

    Plasmid

    #18017

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu D154A Delta13V ECFP-W67G
    

    Use

    Tags

    ECFP-W67G and Venus

    Expression

    Mammalian

    Mutation

    mglB-D154A; non-functional ECFP

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pETM6-M.accABCD

    Plasmid

    #66865

    Purpose

    Encodes accA, accB, accC, accD in monocistronic configuration.

    Depositor

    Mattheos Koffas

    Article

    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

    Inserts

    accA
    accB
    accC
    accD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pFF714

    Plasmid

    #61449

    Purpose

    IPTG-inducible SCRIBE_galK(OFF)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    SCRIBE_galK(OFF)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pFF706

    Plasmid

    #61448

    Purpose

    Light-inducible SCRIBE_kanR(ON)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    SCRIBE_kanR(ON)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pDawn

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pGR-ECK120033127

    Plasmid

    #46011

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Insert

    ECK120033127 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G

    Plasmid

    #18016

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu D154A Delta13V Venus-Y67G
    

    Use

    Tags

    ECFP and Venus-Y67G

    Expression

    Mammalian

    Mutation

    mglB-D154A; non-functional Venus

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAW204

    Plasmid

    #113244

    Purpose

    E. coli expression vector for IHFa F13A + IHFb

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    IHFa F13A (ihfA E. coli)
    IHFb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F13A

    Promoter

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pKDsgRNA-pgaC

    Plasmid

    #154919

    Purpose

    aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting pgaC
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pFF758

    Plasmid

    #61454

    Purpose

    IPTG-inducible msd(wt)_dRT

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    msd(wt)_dRT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    D197A D198A

    Promoter

    pLacO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pECM1

    Plasmid

    #75445

    Purpose

    Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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