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tet_pLKO.1_puro_shNRF2 #1

Plasmid

#136584

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v1 (NFE2L2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMP71-HLA-A0201-His

Plasmid

#108214

Purpose

HLA-A0201 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-A Human)

Use

Retroviral

Tags

his

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLA1

Plasmid

#160807

Purpose

Express POLA1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLA1 (POLA1 Human)

Use

Retroviral

Tags

Expression

Mutation

Codon Optimized

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-P2A-Puro

Plasmid

#110841

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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SYKA-c020

Plasmid

#175489

Purpose

Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

Depositor

Opher Gileadi

Article

Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

Insert

SYK (SYK Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 LRP6-eGFP

Plasmid

#180143

Purpose

to make movies of LRP6 vesicles

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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HA-Rab11-DN (S25N)

Plasmid

#101046

Purpose

Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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LentiGuide-hygro

Plasmid

#160090

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR(Y107A)-Hygro

Plasmid

#176073

Purpose

EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR (Y107A)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Point mutation to convert Try107 to Ala

Promoter

EF1A

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-PHLPP1 full length

Plasmid

#37100

Purpose

Expresses HA tagged, full-length human PHLPP1 in mammalian cells

Depositor

Alexandra Newton

Article

Warfel et al J Biol Chem. 2011 Jun 3;286(22):19777-88

Insert

PHLPP1 (PHLPP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only

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Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP

Plasmid

#170327

Purpose

Double-floxed inverse Orientation HA and IRES-YFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-FNLS-PGK-Puro

Plasmid

#110847

Purpose

Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A and NLS sequence at the N-terminus

Promoter

TRE3G

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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P-rTetO-EGFP-T2A-RPL22-3xHA

Plasmid

#170326

Purpose

Tetracycline-inducible expression of HA and EGFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-BetaActin-SecA5-YFP

Plasmid

#105664

Purpose

Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

Depositor

Qing Deng

Article

Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

Insert

Secreted Annexin5-EYFP (ANXA5 Human)

Use

Tol2-gateway

Tags

EYFP

Expression

Mutation

Promoter

Beta-Actin

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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tet_pLKO.1_puro_shNRF2 #2

Plasmid

#136585

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v2 (NFE2L2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

Plasmid

#170317

Purpose

Expresses HA and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-TK-5UTR-BsmBI-Luciferase

Plasmid

#114670

Purpose

For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter

Depositor

Lynne Postovit

Article

Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi:

Insert

LacZ

Use

Luciferase

Tags

for cloning 5' UTR-Luciferase fusions

Expression

Mammalian

Mutation

Promoter

lac promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-H2B-tdTomato-SRT

Plasmid

#203393

Purpose

AAV transfer plasmid containing H2B fused piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

Depositor

Joseph Dougherty, Rob Mitra

Article

Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

Insert

H2B fused piggyBac tdTomato self-reporting transposon

Use

AAV

Tags

3X SV40 and H2B

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

Plasmid

#170320

Purpose

Expresses HA and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-GCaMP7f-CCR5TC

Plasmid

#216534

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

GCaMP7f

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-rBrokenheart

Plasmid

#203394

Purpose

AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

Depositor

Joseph Dougherty, Rob Mitra

Article

Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

Insert

Brokenheart

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pT2K-LRL-IRES-Luc2

Plasmid

#109230

Purpose

For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

LRL and Luc2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

Plasmid

#170321

Purpose

Expresses HA and mCherry tagged RPL22, neomycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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PiggyBac-FLAG-mProser1-IRES-Blast

Plasmid

#226172

Purpose

Expresses full length mouse PROSER1 with 2X N-terminal FLAG tags

Depositor

Kasper Rasmussen

Article

Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

Insert

Proline Serine Rich protein 1 (Proser1 Mouse)

Use

Tags

2X FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

Plasmid

#170318

Purpose

Expresses FLAG and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo

Plasmid

#170319

Purpose

Expresses HA and EGFP tagged RPL22, neomycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-2X-P2A-Puro

Plasmid

#110840

Purpose

Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-2X

Use

Lentiviral

Tags

Expression

Mutation

D10A and 2 tandem NLS sequences in the XTEN linker

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD6

Plasmid

#195844

Purpose

Expresses human FZD6 with an N-terminal HiBiT; contains M345L

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD6 (FZD6 Human)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

M345L

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLuc.mKO2

Plasmid

#85224

Purpose

shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

Firefly Luciferase

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pUC8 human IFN-gamma

Plasmid

#17600

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

interferon-gamma cDNA (IFNG Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only