We narrowed to 5,258 results for: pcdna3.1

1. pTS2365-Tier1-PhCMV-TetR

   Plasmid

   #169566

   Purpose

   Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven tetracycline repressor protein class B from transposon Tn10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSL2621 (pCas8_Vch)

   Plasmid

   #200893

   Purpose

   Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCas8
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSL2623 (pCas6_Vch)

   Plasmid

   #200895

   Purpose

   Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCas6
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FLAG SMG1 T3550A/S3556A

   Plasmid

   #199574

   Purpose

   Expresses FLAG-tagged mutated SMG1

   Depositor

   Michael Kastan

   Article

   McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

   Insert

   SMG1 (SMG1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T3550A and S3556A

   Promoter

   CMV

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Vh-HA-3xFRB

   Plasmid

   #20223

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   3xFRB (MTOR Human)
   

   Use

   Tags

   HA and Vh

   Expression

   Mammalian

   Mutation

   One copy of FRB has a T2098L mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

6. mc2 blast

   Plasmid

   #206217

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDN42

   Plasmid

   #61345

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-biNLS10 and PKIt NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSL2622 (pCas7_Vch)

   Plasmid

   #200894

   Purpose

   Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCas7
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA-3xFLAG-TCAB1

   Plasmid

   #51630

   Purpose

   Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

   Depositor

   Steven Artandi

   Article

   Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

   Insert

   TCAB1 (WRAP53 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCMV2-hNCOA6:V5

    Plasmid

    #63585

    Purpose

    A subunit of the Trithorax-related (Trr) methyltransferase complex.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

    Insert

    NCOA6 (NCOA6 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

11. VinTS-tCRMod-GGSGGS5

    Plasmid

    #111763

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAL188

    Plasmid

    #22277

    Purpose

    Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    TiamDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EYFP-Clathrin

    Plasmid

    #20921

    Depositor

    Xiaowei Zhuang

    Article

    Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

    Insert

    Clathrin, light polypeptide (Lca) (Clta Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-H2B-Venus

    Plasmid

    #87614

    Purpose

    Venus (YFP) fused to histone H2B.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    H2B-Venus (H2BC8 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mc2 mCherry EF1a

    Plasmid

    #206225

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pspCas9-3'UTR-Tetra-com-vector

    Plasmid

    #132553

    Purpose

    Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA flag-Pcbp2

    Plasmid

    #184028

    Purpose

    Codon optimized Pcbp2 clone with N-terminal flag tag

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Pcbp2 (Pcbp2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    CMV

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FLAG-Fos DD

    Plasmid

    #8968

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S362D, S374D

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pFH2.21_mCherry-MS2x6

    Plasmid

    #205536

    Purpose

    Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-2xFLAG-Atoh1

    Plasmid

    #101734

    Purpose

    Expresses mouse Atoh1 with FLAG tag

    Depositor

    Albert Edge

    Article

    Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HA-EcPPK1

    Plasmid

    #159279

    Purpose

    Expresses a HA-tagged E. coli PPK1 in mammalian cell lines

    Depositor

    Michael Downey

    Article

    Bondy-Chorney et al Cell Rep. 2020 Oct 27;33(4):108318. doi:

    Insert

    HA-tagged Polyphosphate Kinase 1
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. hGFAP R239C

    Plasmid

    #169476

    Purpose

    Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    EGFP-hGFAP (GFAP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed R 239 to C

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EGFP-PH

    Plasmid

    #96948

    Purpose

    EGFP-PLC pleckstrin homology domain for CALI validation

    Depositor

    Marcel Bruchez

    Article

    He et al Nat Methods. 2016 Mar;13(3):263-268. doi

    Insert

    Pleckstrin homology domain
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNIR-FbGFPY58/C-JIP

    Plasmid

    #184684

    Purpose

    NIR-Fb-based JNK inhibitor

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbGFPY58/C-JIP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTS2396-Tier1-PhCMV-4xCOX8_Ypet

    Plasmid

    #169534

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven miochondria-localized yellow fluorescent protein Ypet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. perbB2K732M-ECFP

    Plasmid

    #40269

    Depositor

    Martin Offterdinger

    Article

    Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

    Insert

    erbB2 (ERBB2 Human)
    

    Use

    Tags

    ecfp

    Expression

    Mammalian

    Mutation

    K732M

    Promoter

    CMV

    Available since

    Jan. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-optoB2-SS-YFP

    Plasmid

    #75270

    Purpose

    Arrestin independent version of optoB2 with serine mutations at S362A,S363G

    Depositor

    Michael Bruchas

    Article

    Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

    Insert

    Arrestin independent version of optoB2
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    serine mutations at S362A,S363G

    Promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3 SWS cone opsin

    Plasmid

    #109363

    Purpose

    Human SWS cone opsin with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    SWS cone opsin (OPN1SW Human)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFH2.16_MiniGagZip-MCP_Zip-P2A-eGFP

    Plasmid

    #205531

    Purpose

    Express HIV MiniGagZip-MCP_Zip-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    MiniGagZip-MCP_Zip
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Lmajor_gp63_10_0460_E265A

    Plasmid

    #171641

    Purpose

    Expression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cells

    Depositor

    Dennis Ko

    Article

    Antonia et al Front Cell Infect Microbiol. 2019 Aug 7;

    Insert

    LmjF.10.0460 E265A
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    E265A

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBT140

    Plasmid

    #27493

    Depositor

    Liqun Luo

    Article

    Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

    Insert

    Nuclear localization signal-Cre
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mc2 neo EF1a

    Plasmid

    #206220

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDN41

    Plasmid

    #61344

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS9 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMM585-Tier1-PhCMV-Igk

    Plasmid

    #169486

    Purpose

    Tier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Igk secretion signal (IGK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. BLING 1

    Plasmid

    #171647

    Purpose

    Expresses bioluminescent glutamate indicator in mammalian cells

    Depositor

    Assaf Gilad

    Article

    Petersen et al ACS Synth Biol. 2023 Jul 14. doi: 10.102

    Insert

    BLING 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-HCMVgH (Merlin)

    Plasmid

    #136448

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Venus wGBD

    Plasmid

    #85222

    Purpose

    Venus tagged Cdc-42 sensor

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Insert

    wGBD (WASL Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Cdc42 binding domain of N-WASP

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA-NBid-PhoCl1-CBid

    Plasmid

    #164050

    Purpose

    Optogenetic manipulation of cell apoptosis using PhoCl1

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-PhoCl1-CBid
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 3xFRB-myc

    Plasmid

    #20226

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

40. mc2 blast EF1a

    Plasmid

    #206223

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge