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Showing: 4301 - 4350 of 9382 results

  6. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  8. plRL58

    Plasmid
    #166886
    Purpose
    Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)
    Depositor
    Jeremy Rock
    Article
    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Tet-On
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    166886-plasmid-map-sequence-id-339556

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  33. pRAN473

    Plasmid
    #120813
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1
    Insert
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120813-plasmid-map-sequence-id-235308

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  34. plRL61

    Plasmid
    #163633
    Purpose
    Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).
    Depositor
    Jeremy Rock
    Article
    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Tet-On
    Available Since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163633-plasmid-map-sequence-id-339483

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Showing: 4301 - 4350 of 9382 results