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We narrowed to 12,214 results for: LEA

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Showing: 1721 - 1740 of 12214 results

  6. pGBW-m4046828

    Plasmid
    #145742
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    145742-plasmid-map-sequence-id-298346

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  7. pDNA2.0 6H-FLAG-TEV-KRAS G12C

    Plasmid
    #111848
    Purpose
    Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Publication
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12C (KRAS Human)
    Tags
    6xHis-tag, FLAG-tag, and TEV protease cleavage se…
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Cysteine
    Promoter
    T5
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111848-plasmid-map-sequence-id-213454

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  8. pcDNA3-RAB-EKARev-NLS

    Plasmid
    #118476
    Purpose
    ddRFP-based single-color ERK biosensor targeted to the nucleus.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-EKARev-NLS
    Tags
    6xHIS, Nuclear localization signal (NLS), T7 tag …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    118476-plasmid-map-sequence-id-243128

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  17. nesprin TSmod 25

    Plasmid
    #125555
    Purpose
    Nesprin-2G force sensor with cerulean-F25-venus TSmodule
    Depositor
    Daniel Conway
    Publication
    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mini-nesprin-2G (Syne2 Mouse)
    Tags
    cerulean, venus
    Expression
    Mammalian
    Mutation
    TSmod changed from mTFP to cerulean, flageliform …
    Promoter
    CMV
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125555-plasmid-map-sequence-id-244038

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  18. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    182287-plasmid-map-sequence-id-363047

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  20. pET30a-HS-Tn5

    Plasmid
    #127916
    Purpose
    Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.
    Depositor
    Silin Zhong
    Publication
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Hyperstable Tn5
    Tags
    E coli elongation factor Ts and Mxe intein - Chit…
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127916-plasmid-map-sequence-id-248754

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Showing: 1721 - 1740 of 12214 results