Addgene

We narrowed to 158,806 results for: Gene

Showing: 4401 - 4450 of 158806 results

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pCD227

Plasmid

#113318

Purpose

AraC-pBAD-dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Tags

Expression

Bacterial

Mutation

R93A

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pALACE

Plasmid

#31853

Depositor

Yossef Av-Gay

Article

Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

Type

Empty backbone

Use

Escherichia coli-mycobacterium shuttle plasmid

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

Acetamidase operone

Available since

July 9, 2012

Availability

Academic Institutions and Nonprofits only
iDuet011a

Plasmid

#17628

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

TetO7 - UBC promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pAB164

Plasmid

#101667

Purpose

Opto-T7RNAP*(563), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), half expr.

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pNB648

Plasmid

#60140

Purpose

NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Use

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pRU1161

Plasmid

#14474

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

mRFP1-GusA (uidA Aequorea)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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pNC-BiFC-ED4

Plasmid

#193429

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEV-G3-Ph

Plasmid

#46818

Purpose

TEF1 and GAL10 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Claudia Vickers, Lars Nielsen

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Use

Tags

FLAG and c-myc

Expression

Yeast

Mutation

Promoter

TEF1 and GAL10

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi Bla mAmetrine

Plasmid

#183257

Purpose

Stable expression of blue fluorescent mAmetrine protein in a Sleeping Beauty transposon vector.

Depositor

Alexander McLellan

Article

He et al Int J Mol Sci. 2020 Dec 4;21(23). pii: i

Insert

mAmetrime

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Cabp5 (445bp crit)-tdTomato-IRES-AP

Plasmid

#18812

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Cabp5 (445bp crit)-tdTomato-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pASR102

Plasmid

#122636

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSADdeltaG-GFP-rtTA

Plasmid

#32648

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
rtTA

Use

Rabies virus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
SV40 basal promoter-tdTomato-IRES-AP

Plasmid

#18809

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-tdTomato-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pTRETightBI-RY-HMGA2wt

Plasmid

#31469

Depositor

Phil Sharp

Article

Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

Insert

eYFP and mCherry

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pMG13

Plasmid

#122932

Purpose

Expression of the regulator tTA.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

tTA

Use

Tags

Expression

Yeast

Mutation

Promoter

Pcln3

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCEV-G4-Ph

Plasmid

#46820

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Claudia Vickers, Lars Nielsen

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Use

Tags

FLAG and c-myc

Expression

Yeast

Mutation

Promoter

TEF1 and HXT7

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSADdeltaG-DsRed2

Plasmid

#32638

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

DsRed2

Use

Rabies virus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV AP-4

Plasmid

#12101

Depositor

Robert Tjian

Article

Hu et al Genes Dev. 1990 Oct . 4(10):1741-52.

Insert

AP-4 (TFAP4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pCWU6-xylR-E-lepB

Plasmid

#190725

Purpose

fine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible system

Depositor

Chenggang Wu

Article

Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

Insert

xylR-E-lepB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pXWS20tetO

Plasmid

#160826

Purpose

20 repeats of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(20 X tetO)-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHES875

Plasmid

#89192

Purpose

Expresses mCherry under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pASR130

Plasmid

#122638

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRIT TAP

Plasmid

#17520

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

TAP

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBSKΔB-TRE-dCas9-EGFP

Plasmid

#62347

Purpose

Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

dCas9-EGFP

Use

CRISPR

Tags

HA and SV40 NLS

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
SV40 basal promoter-GFP-IRES-AP

Plasmid

#18807

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-GFP-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
PKC eta CAT

Plasmid

#21247

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC eta (Prkch Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

delta 1-341

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pMAL-c5X-35-H-6

Plasmid

#67008

Purpose

Expresses malE-35-H-6 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-35-H-6

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2774

Plasmid

#160667

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRA03

Plasmid

#103141

Purpose

Triple gRNA expression separated with 28nt Csy4 motif

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

gRNAs

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hHpr1-GST

Plasmid

#11200

Depositor

Robin Reed

Article

Masuda et al Genes Dev. 2005 Jul 1. 19(13):1512-7.

Insert

Hpr1 (THOC1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
M3926 leu2::KanMX3 Disruptor Converter

Plasmid

#51680

Purpose

Yeast marker swap plasmid for converting LEU2 to KanMX3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pNTC OSCAR

Plasmid

#29641

Depositor

David_L Andrews, Scott Gold

Article

Paz et al Fungal Genet Biol. 2011 Mar 5. ():.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pJMP2836

Plasmid

#160674

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p2Lox

Plasmid

#34635

Depositor

Michael Kyba

Article

Iacovino et al Stem Cells. 2011 Oct;29(10):1580-8.

Type

Empty backbone

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

PGK promoter

Available since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pQFT-lacZ

Plasmid

#208896

Purpose

pQFT with lacZ under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Contains two silent mutations and a missense muta…

Promoter

PQJ

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCVhyg-3xT7

Plasmid

#171028

Purpose

Expresses 3xT7-tagged target in mammalian cells

Depositor

Kai Ge

Article

Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

Type

Empty backbone

Use

Retroviral

Tags

3x-T7

Expression

Mammalian

Mutation

Promoter

5' LTR

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-2A-TAL6-MSTN

Plasmid

#72597

Purpose

MSTN TALEN with 2A-GFP tagged

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

TALEN for MSTN

Use

TALEN

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB783

Plasmid

#60162

Purpose

Firefly luciferase (Promega) driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

A4V & S504G (no functional effect)

Promoter

RNR1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBFv-U6.2B

Plasmid

#138401

Purpose

gRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vector

Depositor

Shu Kondo

Article

Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

Insert

U6-2

Use

Tags

Expression

Insect

Mutation

Promoter

U6-2

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A5801

Plasmid

#127223

Purpose

35S::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NAT-TET-OFF -MET - 3xHA

Plasmid

#182526

Purpose

Doxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

7 Copies of TetO Box

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A5803

Plasmid

#127224

Purpose

CmYLCV::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RSV CREB

Plasmid

#22394

Depositor

Marc Montminy

Article

Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

Insert

CREB (Creb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
Hy_pMT EGFP SV40 pA

Plasmid

#69911

Purpose

Expresses EGFP in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNeDaKO-Hyg

Plasmid

#16414

Depositor

Bert Vogelstein

Article

Kohli et al Nucleic Acids Res. 2004 . 32(1):e3.

Insert

PGK-HygR & ZeoR

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-Ii31

Plasmid

#64598

Purpose

Expression of invariant chain as model antigen for study of MHC class II presentation

Depositor

Martin Zenke, Sandra Diebold

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

MHC II Invariant Chain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pXWS10tetO

Plasmid

#160825

Purpose

10 repeats of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(10 X tetO)-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Rad18

Plasmid

#119003

Purpose

Mammalian expression of human Rad18 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Rad18

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSADdeltaG-GCaMP3

Plasmid

#32644

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

GCaMP3

Use

Rabies virus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2824

Plasmid

#160671

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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