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pTYF-super-mfCCK-0.5k-Venus-WPRE

Plasmid

#156392

Purpose

Expresses Venus under CCK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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untargeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45215

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Aurora B FRET sensor

Tags

CyPET, YPET

Expression

Mammalian

Promoter

t7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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pMAL-BRT17

Plasmid

#45358

Depositor

Scott Banta

Article

Shur et al Biotechniques. 2013 Apr;54(4):197-206. d

Insert

17 Repeat Beta Roll Tag

Tags

Maltose Binding Protein (MBP)

Expression

Bacterial

Promoter

Ptac

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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pETM33_SIN3A_PAH

Plasmid

#178486

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SIN3A_PAH (SIN3A Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-yomRuby3-SpHIS5

Plasmid

#204287

Purpose

For C-terminal tagging using yeast optimized mRuby3

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pFA6-yomRuby3-SpHIS5

Tags

yomRuby3

Expression

Yeast

Promoter

none

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pscALPSblasti-TMPRSS2 Blasti

Plasmid

#158088

Purpose

Expresses TMPRSS2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP2B

Plasmid

#183325

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2B gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2B

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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3xflag-APEX2-NES

Plasmid

#164623

Purpose

Expresses a nuclear exporting signal (NES) tagged with APEX2 and 3xflag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

NES

Tags

3xFLAG and APEX2

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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Histone H2B targeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45216

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

Histone H2B-targeted Aurora B FRET sensor

Expression

Mammalian

Promoter

T7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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pYS2

Plasmid

#117490

Purpose

Generation of unmarked gene deletions in Mycobacterium smegmatis

Depositor

Eyal Gur

Article

Shenkerman et al Gene. 2014 Jan 1;533(1):374-8. doi: 10.1

Insert

gfp, hyg

Use

Deletion generation

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBMN-MEF2C

Plasmid

#232627

Purpose

Lentiviral plasmid expressing mouse MEF2C.

Depositor

Claes Wadelius

Article

Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

Insert

MEF2C (Mef2c Mouse)

Use

Lentiviral

Tags

HA-3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ATF4

Plasmid

#192911

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ATF4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ATF4 (ATF4 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_Z17-ehaA_LacI

Plasmid

#186313

Purpose

Plasmid for inducible E coli expression of the Z17 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

Z17-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC29A1

Plasmid

#131927

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC29A1 (SLC29A1 Human)

Expression

Mammalian

Available Since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pJFRC153-20XUAS-IVS-B2::PEST

Plasmid

#32134

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B2::PEST

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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CENP-B targeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45214

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

CENP-B targeted Aurora B FRET sensor

Expression

Mammalian

Promoter

t7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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pGWF1

Plasmid

#18856

Depositor

Wolf Frommer

Article

Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

Type

Empty backbone

Tags

CFP and Venus

Expression

Bacterial

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_Z18-ehaA_LacI

Plasmid

#186314

Purpose

Plasmid for inducible E coli expression of the Z18 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

Z18-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINEneo-3C-GFP

Plasmid

#53534

Purpose

pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system

Depositor

Ray Owens

Insert

GFP

Tags

GFP-His8

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA

Plasmid

#140221

Purpose

Constitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

dLbCpf1 (Cas12a) N terminal half (406) with zipper

Expression

Mammalian

Promoter

PGK

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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