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Plasmid#206025PurposeAn expression vector for mouse LINE1 ORF1DepositorInsertORF1 derived from mouse LINE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry Tshld-GFP
Plasmid#53211PurposepEntry Tshld-GFP is a product of BP recombination reaction between attB flanked Tshld system-encoded PCR product and pDonor 201 plasmid.DepositorInsertTshld-GFP
UseTagsEGFP, Flag, and HAExpressionMutationPromoternoneAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas9–mKate2ps–CgRNA
Plasmid#64955PurposeControl gRNA (GTCAAGGCACTCTTGCCTA)DepositorInsertCas9–mKate2ps
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJC20cdc8-cerulean3
Plasmid#99358PurposeBacterial expression of Cerulean3 fused to the 3' of cDNA of the fission yeast tropomyosin, cdc8.DepositorInsertcdc8 (cdc8 Fission Yeast)
UseTagsCerulean3 and Gly3-His6ExpressionBacterialMutationPromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET16
Plasmid#107442PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET16 (MET16 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET10
Plasmid#107440PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET10 (MET10 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFGL1258
Plasmid#116898PurposeTetOFF:GFP:CenpA (centromere marker)DepositorInsertsCenpA promoter region
CenpA
UseFungal expression (in magnaporthe oryzae)TagsExpressionMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMYs-IRES-mitoRFP
Plasmid#121996PurposeExpression of mitoRFP in mammalian cellsDepositorInsertIRES-mitoRFP
UseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL50015
Plasmid#50318PurposePlease note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)DepositorInsertCDS, mNEON (Branchistoma lanceolatum)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenLox_U6 Nlgn3
Plasmid#59359PurposeLentiviral expression vector: Neuroligin 3 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.DepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterCAG and mouse U6Available sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV.PARP1#3
Plasmid#14546DepositorInsertPARP1 shRNA (PARP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMLC-BB1
Plasmid#166922PurposeBackbone for assembly of multi-level gene expression controllersDepositorInsertCyOFP - Orange fluorescent protein - flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100 constitutiveAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL2_164
Plasmid#199096PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpIP501 ORI plus NGS barcode 4, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_165
Plasmid#199097PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpSC101ts ORI plus NGS barcode 10, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_161
Plasmid#199093PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpAL5000 ORI plus NGS barcode 1, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_162
Plasmid#199094PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpUB110 ORI plus NGS barcode 2, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH87633
Plasmid#50271DepositorInsertpromoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorInsertSpCas9-p (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHS
Plasmid#166938PurposeToehold switch multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Toehold switch
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHES841
Plasmid#89194PurposeExpresses YFP under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-YFP
UseTagsExpressionYeastMutationPromoterpZAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES874
Plasmid#89196PurposeExpresses mCherry under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-mCherry
UseTagsExpressionYeastMutationPromoterpZAvailable sinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPYCdKN0001
Plasmid#196148PurposeGateway destination vector for protein expression of 9xHis_AtTGA1_HiBitDepositorInsert9xHis_AtTGA1_HiBit
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMLS253
Plasmid#114271PurposeSapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)DepositorInsertCbr-Unc-119
UseCRISPR and Cre/LoxTagsExpressionMutation*please see depositor comment belowPromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p123shy1-3HA
Plasmid#67876Purposeencodes shy1 with a C-terminal 3xHA-tag under control of its endogenous promoter.DepositorInsertshy1
UseExpression in ustilago maydisTagsHAExpressionMutationPromotershy1Available sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenLox_U6 Nlgn1
Plasmid#59339PurposeLentiviral expression vector: Neuroligin 1 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.DepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterCAG and mouse U6Available sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
DT037A
Plasmid#159398PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoterDepositorInsertpr.J23101-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterJ23101 (strong) promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Banshee-Sh-Ctrl
Plasmid#85587PurposeControl hairpin sequence used in gene knockdown experiementsDepositorInsertsh-Ctrl
UseRetroviralTagsExpressionMammalianMutationPromoterH1Available sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2_174
Plasmid#199106PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpNG2 ORI plus NGS barcode 22, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_177
Plasmid#199109PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpBC1 ORI plus NGS barcode 25, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL70002
Plasmid#50333DepositorInsertBiotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL0_17 [AMA1 short ORI]
Plasmid#198930PurposeLevel 0 partDepositorInsertAMA1 short ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-H2B-mCherry
Plasmid#135604PurposeExpression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratusDepositorInsertH2B-mCherry
UseIn vitro transcription (mrna synthesis)TagsmCherryExpressionMutationPromoterUnknownAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHES834
Plasmid#87947PurposeExpresses mKate2 under control of GAL1 promoter.DepositorInsertpGAL1-mKate2
UseSynthetic BiologyTagsExpressionYeastMutationPromoterpGal1Available sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAct-dCBEevoAPOBEC1
Plasmid#195514PurposeUbiquitous expression of dCBEevoAPOBEC1 in DrosophilaDepositorInsertdCBE-evoAPOBEC1
UseCRISPRTagsExpressionInsectMutationPromoteract5CAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
myc-NLRC5 K234A
Plasmid#37523DepositorInsertNLRC5 (NLRC5 Human)
UseTagsmycExpressionMammalianMutationK234APromoterCMVAvailable sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-TO-Myc-rZAP
Plasmid#17381DepositorInsertZAP (Zc3hav1 Rat)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
RCASBP-AP (E) (CC#39)
Plasmid#15162DepositorInsertPLAP (ALPP Human)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pICH51277
Plasmid#50268DepositorInsertpromoter (0.4 kb), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPYCeGM0001
Plasmid#196141PurposeGateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tagDepositorInsertAtTGA1_HiBit
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -