We narrowed to 7,279 results for: gnal

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pcDNA3.2/pr-Membrane

Plasmid

#120817

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

pr-Membrane ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Mito-SNAP

Plasmid

#206480

Purpose

Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)

Tags

SNAPf

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pET-26b-Nb127D01-ALFA-His

Plasmid

#171568

Purpose

Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-ALFA-His (CXCR2 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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BB3cK_pGAP_23*_pLAT1_Cas9

Plasmid

#104908

Purpose

hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PH-Btk-mRuby2

Plasmid

#124657

Purpose

PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

BTK (BTK Human)

Tags

mRuby2

Expression

Mammalian

Mutation

contains only the PH domain of Bruton tyrosine ki…

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

Plasmid

#63565

Purpose

Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysines at 5, 9, 118, 119, 125, 127, and 129 are…

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
Wnt3A STOP

Plasmid

#35871

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK15

Plasmid

#23697

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK15 (MAPK15 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
IMS-HyPer2

Plasmid

#60248

Purpose

Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

IMS-HyPer2

Tags

Mitochondrial intermembrane space targeting signa…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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GBSGFP insulator

Plasmid

#121975

Purpose

Gli activity reporter, mouse transgenesis

Depositor

James Briscoe

Article

Balaskas et al Cell. 2012 Jan 20;148(1-2):273-84. doi:

Insert

GBS(long)-GFP-insulator (Gnb5 Mouse)

Use

Mouse transgenesis

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only
pMH4-SYN-EGFP-ER

Plasmid

#22285

Depositor

Thomas Oertner

Article

Holbro et al Proc Natl Acad Sci U S A. 2009 Sep 1;106

Insert

EGFP-ER

Tags

EGFP-ER

Expression

Mammalian

Mutation

EGFP alanine 206 changed to Lysin

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pGFH-9

Plasmid

#39538

Depositor

Katharina Strub

Article

Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

Insert

SRP9 (SRP9 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L S468A

Plasmid

#27037

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Tags

HA

Expression

Mammalian

Mutation

S468A

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pCMV7.1_3xFLAG-LATS2

Plasmid

#172987

Purpose

constitutive expression of FLAG-tagged LATS2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-LATS2 (LATS2 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLck-mVenus-C1

Plasmid

#84337

Purpose

Expresses a mVenus tagged with a membrane localization sequence

Depositor

Dorus Gadella

Article

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Insert

Lck (MGCVCSSNPELPVAT)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Wnt1 STOP

Plasmid

#35868

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1-mPRAS40

Plasmid

#15480

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 shRNA (Akt1s1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-YFP-STM1(24-685) EF hand 3 mut (A79K, N80D, D82G)

Plasmid

#18865

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal interaction molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

3 mutations (A79K, N80D, D82G) in the EF hand of …

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

Plasmid

#89587

Purpose

For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) turboRFP

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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