4,203 results

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pAAV-mDlx-GCaMP6f-Fishell-2

Plasmid

#83899

Purpose

GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pLX303

Plasmid

#25897

Purpose

3rd generation lentiviral Gateway destination vector. Blasticidin selection.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Expression

Mammalian

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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Stat3-C Flag pRc/CMV

Plasmid

#8722

Depositor

Jim Darnell

Article

Bromberg et al Cell 1999 Aug 6;98(3):295-303.

Insert

Stat3-C (Stat3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

A662C and N664C. Residues changed to Cys renders…

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_LaG16_2_synNotch_Gal4VP64

Plasmid

#85421

Purpose

Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

LaG16_2 GFP nanobody synNotch Gal4VP64

Use

Lentiviral

Promoter

PGK

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEYFP-C1-Drp1

Plasmid

#45160

Depositor

Richard Youle

Article

Frank et al Dev Cell. 2001 Oct;1(4):515-25.

Insert

Drp1 (DNM1L Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6

Plasmid

#39296

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pET-GST

Plasmid

#42049

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

GST

Tags

His

Expression

Bacterial

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ClopHensor

Plasmid

#25938

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

ClopHensor

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP is EGFP-T203Y

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt3A

Plasmid

#35908

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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HA-VHL-pRc/CMV

Plasmid

#19999

Depositor

William Kaelin

Article

Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pCAGGS-ChR2-Venus

Plasmid

#15753

Purpose

Expresses ChR2-Venus for neuronal excitation.

Depositor

Karel Svoboda

Article

Petreanu et al Nat Neurosci. 2007 May . 10(5):663-8.

Insert

Channelrhodopsin2-Venus

Tags

Venus

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has Service

Cloning Grade DNA

Insert

mClover3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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GW1-Mito-pHRed

Plasmid

#31474

Depositor

Gary Yellen

Article

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

Cox8-tagged pHRed

Tags

4xCox8

Expression

Mammalian

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti6-MK1-EHMT2-V5

Plasmid

#31113

Depositor

Bernard Futscher

Insert

euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Stop codon mutated to Ala

Available Since

Aug. 8, 2011

Availability

Academic Institutions and Nonprofits only

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Paxillin-pEGFP

Plasmid

#15233

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

paxillin (PXN Chicken)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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7TGC

Plasmid

#24304

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-eGFP // SV40-mCherry

Use

Lentiviral

Mutation

EGFP is GFP with S65T and F64L mutations

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP11

Plasmid

#22566

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pMXs-3XFLAG-EGFP-OMP25

Plasmid

#83354

Purpose

For tagging mitochondria with Sigma FLAG epitopes

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XFLAG-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ104 - Pmyo-3::mCherry::unc-54

Plasmid

#19328

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-3::mCherry

Tags

mCherry

Expression

Worm

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Plasmid

#41863

Purpose

This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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p415-GalL-Cas9-CYC1t

Plasmid

#43804

Purpose

A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

Depositor

George Church

Article

Dicarlo et al Nucleic Acids Res

Insert

Human Optimized S. pyogenes Cas9

Use

CRISPR

Expression

Yeast

Promoter

GalL

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-tdPP7-3xmCherry

Plasmid

#74926

Purpose

Expresses tdPP7-3xmCherry

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

PP7-3xmCherry

Use

Lentiviral

Expression

Mammalian

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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FUtdTW

Plasmid

#22478

Depositor

Connie Cepko

Article

Rompani et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

tandem dimer tomato

Use

Lentiviral

Expression

Mammalian

Promoter

UbC

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24

Plasmid

#28138

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pMW#3

Plasmid

#13350

Depositor

Marian Walhout

Article

Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

Insert

LacZ reporter

Expression

Yeast

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-RhoA

Plasmid

#23224

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoA (RHOA Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
GFP-Foxo1

Plasmid

#17551

Depositor

Domenico Accili

Article

Frescas et al J Biol Chem. 2005 May 27. 280(21):20589-

Insert

pEGFP-N1 Foxo1 (Foxo1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2008

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta K85A pcDNA3

Plasmid

#14755

Depositor

Jim Woodgett

Article

Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Insert

GSK3 beta K85A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

K85A. Kinase dead mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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4,203 results