We narrowed to 12,106 results for: LEA
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Plasmid#124661PurposeExpresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coilDepositorInsertSpyCatcher002-oDi
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-DogCatcher
Plasmid#171929PurposeExpress Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.DepositorInsertCys-DogCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3-TPC2
Plasmid#80153PurposeIntracellular Ca2+ store markerDepositorAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EGFP-Ahi1
Plasmid#30494DepositorAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET GFP domain 11 LIC cloning vector (GFP C-11)
Plasmid#30183DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), GFP domain 11, and TEV cl…ExpressionBacterialAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pX330S-6
Plasmid#58782PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-RanWT-mRFP1-polyA
Plasmid#59750PurposeExpress wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertRan-mRFP1
ExpressionMammalianPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMBP-AaCas12b
Plasmid#113433PurposeExpresses Expresses 10xHis-MBP fused to AaCas12bDepositorInsertAaCas12b
TagsMBPExpressionBacterialPromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pErCas12a EZ Clone
Plasmid#132641PurposeExpresses ErCas12a and an sgRNA to target a region of interestDepositorInsertErCas12a
UseAAV and CRISPRTags3x HA and SV40 NLSExpressionMammalianMutationQ926R (Please see depositor comments) and BsaI si…PromoterCMV PromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBUN6I11
Plasmid#50580PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorInsertsdCas9-KRAB
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSMutationdCas9 that is defective in DNA cleavage; the maiz…PromoterOsU3p and Ubi1pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA6-N-Myc-SCAP
Plasmid#134285PurposeExpresses Myc-tagged SCAP in mammalian cellsDepositorAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
ExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDG1-PHP.eB
Plasmid#220799PurposeAAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genesDepositorInsertE4, E2A, VA, AAV2 rep and PHP.eB cap genes
UseAAV; Aav helper vectorExpressionMammalianAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGSHU
Plasmid#72244PurposeContains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
hyg
I-SceI
ExpressionBacterialAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ad-Flag-XBP1s
Plasmid#63678PurposeAdenoviral vector for XBP1s expressionDepositorAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-ExRai-AMPKAR(T/A)
Plasmid#192447PurposeNon-phosphorylatable negative-control mutant of ExRai-AMPKAR.DepositorInsertExRai-AMPKAR(T/A)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationThr 6 in AMPK substrate mutated to Ala.PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEJS1099: Dual-sgRNA.Design 4
Plasmid#159537PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 with two guide RNA cassettes
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PLAA
Plasmid#85669PurposeExpression of PLAA with N-terminal GFP tagDepositorAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ITCH
Plasmid#36197PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertITCH (ITCH Human)
TagsHis tag with TEV cleavageExpressionBacterialMutationcontains HECT domainAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pOpen-T5gene12/13
Plasmid#165559PurposeDouble-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction.DepositorInsertT5 Exonuclease
UseSynthetic BiologyAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits