We narrowed to 15,732 results for: LEA

1. pJFRC7-UAS-NPRR_ANP

   Plasmid

   #133243

   Purpose

   Fluorescent reporter for neuropeptide release imaging

   Depositor

   David Anderson

   Article

   Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

   Insert

   rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
   

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMBPCas2/3

   Plasmid

   #89238

   Purpose

   Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas2/3
   

   Tags

   Strep II-MBP

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV HDM2(NES-)

   Plasmid

   #12087

   Depositor

   Tyler Jacks

   Article

   Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

   Insert

   HDM2 (MDM2 Human)
   

   Expression

   Mammalian

   Mutation

   Nuclear-export sequence mutant (NES-): L205A, I20…

   Available Since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pAcGFP-Jpx9C104T

   Plasmid

   #120381

   Purpose

   Reporter for RNA nuclear localization

   Depositor

   Igor Ulitsky

   Article

   Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

   Insert

   JPX (JPX Human)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   C321T

   Promoter

   CMV

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. NKP64 GFP (1007)

   Plasmid

   #62020

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

   Insert

   SLC38A10 (SLC38A10 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

6. HisMBP CFlm25

   Plasmid

   #78458

   Purpose

   Inducible expression in E. coli

   Depositor

   Sylvie Doublie

   Article

   Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-

   Insert

   Human Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
   

   Tags

   His and MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCLR2070

   Plasmid

   #28063

   Depositor

   Keith Joung

   Article

   Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

   Insert

   FokI endonuclease
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains N496D point mutation in FokI domain, as …

   Available Since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCLR2068

   Plasmid

   #28062

   Depositor

   Keith Joung

   Article

   Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

   Insert

   FokI endonuclease
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains H537R point mutation in FokI domain, as …

   Available Since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046921

   Plasmid

   #145755

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pUPD2+pCmYLCV911

    Plasmid

    #170878

    Purpose

    Phytobrick - pCmYLCV911

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    pCmYLCV911
    

    Expression

    Plant

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. NET39 pEGFP-C1 (1045)

    Plasmid

    #62024

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET39 (PPAPDC3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pGBW-m4046854

    Plasmid

    #145759

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 leader protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. CD44I-c001

    Plasmid

    #175448

    Purpose

    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcGFP-Jpx9

    Plasmid

    #120379

    Purpose

    Reporter for RNA nuclear localization

    Depositor

    Igor Ulitsky

    Article

    Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

    Insert

    JPX (JPX Human)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMaCTag-Z03

    Plasmid

    #120046

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    moxCerulean3
    

    Use

    Pcr template

    Tags

    moxCerulean3

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. NET39 mRFP-C1 (1219)

    Plasmid

    #62042

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET39 (PPAPDC3 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

17. H2B-mScarlet- hMeikin(1-332, E151R, R154E)-mNeonGreen

    Plasmid

    #174715

    Purpose

    Stable expression of fluorescent Separase cleavage sensor with human Meikin with Separase-resistant mutations

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin
    

    Use

    Retroviral

    Tags

    H2B-mScarlet and mNeonGreen

    Expression

    Mammalian

    Mutation

    1-332, E151R, R154E

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAcGFP-Jpx9C104A

    Plasmid

    #120380

    Purpose

    Reporter for RNA nuclear localization

    Depositor

    Igor Ulitsky

    Article

    Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

    Insert

    JPX (JPX Human)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    C321A

    Promoter

    CMV

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBW-m4046839

    Plasmid

    #145646

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGBW-m4046770

    Plasmid

    #145594

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 leader protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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