164,577 results

1. pHR-tdMCP-YFP

   Plasmid

   #99151

   Purpose

   Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   Dimeric MS2-coat binding protein
   

   Use

   Lentiviral

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

   Plasmid

   #208942

   Purpose

   The CE1 construct, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKDsgRNA-ack

   Plasmid

   #62654

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 5xGAL4-TATA-luciferase

   Plasmid

   #46756

   Purpose

   Luciferase reporter gene for factors with GAL4 DNA binding domains

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   minimal TATA

   Available Since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. p6XHis-T7(P266L)

   Plasmid

   #174866

   Purpose

   Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

   Depositor

   Anna Pyle

   Article

   Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

   Insert

   T7 RNA polymerase
   

   Use

   Tags

   6XHis-tag

   Expression

   Bacterial

   Mutation

   P266L

   Promoter

   lac promoter

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pANAP

   Plasmid

   #48696

   Purpose

   Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

   Depositor

   Peter Schultz

   Article

   Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

   Inserts

   8 copies of the amber suppressor tRNACUA-Leu
   AnapRS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutant E.coli leucyl synthetase specific for Anap…

   Promoter

   CMV and human H1 promoter

   Available Since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHCMV-AmphoEnv

   Plasmid

   #15799

   Depositor

   Miguel Sena-Esteves

   Article

   Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

   Insert

   4070A Amphotropic Murine leukemia virus envelope gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCXWB-EBNA1

   Plasmid

   #37624

   Purpose

   Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   EBNA-1 (EBNA-1 human herpesvirus 4)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKOR1

   Plasmid

   #133446

   Purpose

   Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection

   Depositor

   Olaf Schneewind, Taeok Bae

   Article

   Bae et al Plasmid. 2006 Jan;55(1):58-63. doi: 10.1

   Type

   Empty backbone

   Use

   E.coli/s.aureus shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATG9A-mCherry

    Plasmid

    #197393

    Purpose

    Expresses ATG9A in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiGuide-mCherry

    Plasmid

    #217005

    Purpose

    lentiviral plasmid for gRNA cloning with selectable mCherry expression

    Depositor

    Deepta Bhattacharya

    Article

    D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV8R-12 Capsid

    Plasmid

    #213968

    Purpose

    AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

    Depositor

    Zhonghua Lu

    Article

    Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28 His6-mEGFP-D4H

    Plasmid

    #226373

    Purpose

    Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4H (pfoA Clostridium perfringens)
    

    Use

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSPgRNA

    Plasmid

    #47108

    Purpose

    Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    SPgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM60

    Plasmid

    #34610

    Depositor

    David Sabatini

    Insert

    deptor (DEPTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pLenti-SF3B1-K700E-reporter

    Plasmid

    #200318

    Purpose

    A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Insert

    BSD-2A-EGFPi
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FH-Tet2-pEF

    Plasmid

    #41710

    Purpose

    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

    Insert

    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

    Plasmid

    #221235

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLTR-RD114A

    Plasmid

    #17576

    Depositor

    Jakob Reiser

    Article

    Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

    Insert

    RD114 envelope glycoprotein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 14, 2008

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pBbA6c-RFP

    Plasmid

    #35290

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LlacO-1

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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