We narrowed to 39,201 results for: ANT

1. pGreenII 0229/ProRUBY:GOXL2-Citrine:NOSt

   Plasmid

   #175564

   Purpose

   pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-2

   Depositor

   George Haughn

   Article

   Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

   Insert

   GOXL2
   

   Use

   Tags

   Citrine

   Expression

   Plant

   Mutation

   Promoter

   RUBY

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVITRO1-dV-IgE/κ

   Plasmid

   #61878

   Purpose

   Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin Epsilon constant region
   Human immunoglobulin kappa constant region
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   mEF1 Prom and rEF1 Prom

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVITRO1-dV-IgE/λ

   Plasmid

   #61879

   Purpose

   Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin Epsilon constant region
   Human immunoglobulin lambda constant region
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   mEF1 Prom and rEF1 Prom

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVITRO1-dV-IgG1/κ

   Plasmid

   #52213

   Purpose

   Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin gamma 1 constant region
   Human immunoglobulin kappa constant region
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVITRO1-dV-IgG1/λ

   Plasmid

   #52214

   Purpose

   Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin gamma 1 constant region
   Human immunoglobulin lambda constant region
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGB 35s:dCas:BRD:tNos (GB1172)

   Plasmid

   #75401

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas9:BRD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. K785R Smarca4-sfGFP

   Plasmid

   #107057

   Purpose

   Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

   Depositor

   Courtney Hodges, Jerry Crabtree

   Article

   Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
   

   Use

   Lentiviral

   Tags

   Super-folder GFP (sfGFP)

   Expression

   Mammalian

   Mutation

   K785R

   Promoter

   CAG

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. FcD265A-FLAG

   Plasmid

   #65420

   Purpose

   Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production

   Depositor

   Dane Wittrup

   Article

   Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

   Insert

   FcD265A-FLAG
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D265A

   Promoter

   Modified CMV Promoter

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

9. K785R Smarca4-V5 IRES-puro

   Plasmid

   #107059

   Purpose

   Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

   Depositor

   Courtney Hodges, Jerry Crabtree

   Article

   Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-puroR and V5

   Expression

   Mammalian

   Mutation

   K785R

   Promoter

   CAG

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Raichu-OsRac1 DN

    Plasmid

    #133263

    Purpose

    Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(T24N) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 T24N

    Promoter

    Ubi

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGPN-ACE2

    Plasmid

    #187483

    Purpose

    Expression vector - ACE2

    Depositor

    Lewis Cantley

    Article

    Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1D3 HC

    Plasmid

    #170670

    Purpose

    Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 HC (Human)

    Depositor

    Rafel Radi, Ronald Jemmerson

    Article

    Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

    Insert

    HC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. 1D3 LC

    Plasmid

    #170669

    Purpose

    Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 LC (Human)

    Depositor

    Rafel Radi, Ronald Jemmerson

    Article

    Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

    Insert

    LC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid

    #68223

    Purpose

    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    35S

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGB3 alpha1 35s:PIF6:tNos (GB0170)

    Plasmid

    #160606

    Purpose

    TU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    PIF6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWZL-hygro-FAKP712/713A

    Plasmid

    #216544

    Purpose

    This retroviral plasmid expresses a mutated cDNA (dominant negative: P712/713A) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (P712/713A) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK dominant negative mutation P712/713A

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWZL-hygro-FAKS732A/S910A

    Plasmid

    #221642

    Purpose

    This retroviral plasmid expresses a double-mutated cDNA (dominant negative: S732A/S910A) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (S732A/S910A) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK double mutation S732A/S910A (dominant negativ…

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWZL-hygro-FAKR177/178A

    Plasmid

    #216543

    Purpose

    This retroviral plasmid expresses a mutated cDNA (dominant negative: R177/178A) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (R177/178A) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK dominant negative mutation R177/178A

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSB278 - pL0_pT16H2 (pro + 5U)

    Plasmid

    #203889

    Purpose

    T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    T16H2 promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUN1224 - pL0_pT3R (pro + 5U)

    Plasmid

    #203892

    Purpose

    T3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    T3R promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUN1215 - pL0_pDAT (pro + 5U)

    Plasmid

    #203895

    Purpose

    DAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    DAT promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSB55 - pL0_CrGLK (CDS)

    Plasmid

    #203903

    Purpose

    MoClo compatible L0 plasmid containing the CrGLK coding sequence

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    CrGLK
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Silent mutation of BpiI or BsaI cut site for MoCl…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFUSEss-CHIg-mG3_CH2charge

    Plasmid

    #105858

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG3 heavy chain (Ighg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutated heavy chain of mouse IgG3, muatations: Hi…

    Promoter

    hEF1-HTLV prom

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHSN6I01

    Plasmid

    #50587

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-KRAB
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Expression

    Mutation

    dCas9 that is defective in DNA cleavage; the maiz…

    Promoter

    2×35Sp and AtU6-26p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHSN6A01

    Plasmid

    #50586

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-VP64
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Expression

    Mutation

    dCas9 = defective in DNA cleavage; 4×minimal VP16…

    Promoter

    2×35Sp and AtU6-26p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBUN6A11

    Plasmid

    #50579

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-VP64
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Expression

    Mutation

    dCas9 = defective in DNA cleavage; 4×minimal VP16…

    Promoter

    OsU3p and Ubi1p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBUN6I11

    Plasmid

    #50580

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-KRAB
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Expression

    Mutation

    dCas9 that is defective in DNA cleavage; the maiz…

    Promoter

    OsU3p and Ubi1p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHSN501

    Plasmid

    #50589

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    zCas9D10A
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Cas9D10A nickase, was derived from the zCas9 and …

    Promoter

    2×35Sp and AtU6-26p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBUN501

    Plasmid

    #50582

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    zCas9D10A
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Cas9D10A nickase, was derived from the zCas9 and …

    Promoter

    AtU6-26p and Ubi1p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSB63 - pL0_fLUC-I (CDS1)

    Plasmid

    #123180

    Purpose

    Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
    

    Use

    Luciferase; Part for plant expression

    Tags

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge