163,606 results

1. pLentiFUCCI(CA)5

   Plasmid

   #223176

   Purpose

   Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

   Depositor

   Neville Sanjana

   Article

   Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

   Insert

   FUCCI(CA)5
   

   Use

   Lentiviral

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Flag hHMGB1

   Plasmid

   #31609

   Depositor

   Yasuhiko Kawakami

   Article

   Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

   Insert

   HMGB1 (HMGB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBZS-M.CviPII (NDel15)

   Plasmid

   #198356

   Purpose

   Express M.CviPII (NDel15) in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   M.CviPII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cavb3

   Plasmid

   #26574

   Depositor

   Diane Lipscombe

   Insert

   calcium channel beta subunit-III (Cacnb3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEmerald-Sec23A

   Plasmid

   #166893

   Purpose

   mammalian expression of Sec23A tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   SEC23A (SEC23A Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

   Plasmid

   #183790

   Purpose

   A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Inserts

   P2A-eGFP-TargetBC-5xTAPE-1
   pegRNA-InsertBC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   G>A in the gRNA scaffold- please see depositor…

   Promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. px459V2.0_Conc2

   Plasmid

   #134451

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9n(BB)-2A-GFP (PX461)

   Plasmid

   #48140

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-HA-eLACCO2.1-NGR

   Plasmid

   #208023

   Purpose

   Biosensor for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   eLACCO2.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Cas9-GFP_sg_mAMPKa1

    Plasmid

    #79004

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa1 (Prkaa1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCWXPGR-pTF-betaCatenin

    Plasmid

    #114281

    Purpose

    TET-inducible expression of a constitutively active form of the BetaCatenin protein

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    Catenin beta 1 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    deletion of AA 1 to 45

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBT281

    Plasmid

    #122611

    Purpose

    express evoAPOBEC1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Promoter

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cas9-GFP_sg_mAMPKa2

    Plasmid

    #79005

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa2 (Prkaa2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-FLEX-tdTomato

    Plasmid

    #28306

    Depositor

    Edward Boyden

    Has Service

    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    tdTomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPlatTET-gRNA2

    Plasmid

    #82559

    Purpose

    All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo

    Plasmid

    #136904

    Purpose

    Lentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    mScarletGo-p2a-Blas
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC18R6KT-mini-Tn7T-Km

    Plasmid

    #64969

    Purpose

    mini-Tn7 base vector with transcriptional terminator and oriT

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. ER-mNeonGreen

    Plasmid

    #137804

    Purpose

    Visualization of the Endoplasmic Reticulum

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FSW_jGCaMP8f

    Plasmid

    #197034

    Purpose

    Express GCaMP8f from hSyn1 promoter

    Depositor

    Ronald Hart

    Insert

    GCaMP8f
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FUW

    Plasmid

    #14882

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-WRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. spMSP1D1

    Plasmid

    #173482

    Purpose

    Expresses spMSP1D1 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spMSP1D1
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIVM_02

    Plasmid

    #204500

    Purpose

    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

    Depositor

    Alessio Ciulli

    Article

    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

    Insert

    amino acids 54-213 VHL (VHL Human)
    

    Use

    Tags

    6xHis, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    0

    Promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pXR001: EF1a-CasRx-2A-EGFP

    Plasmid

    #109049

    Purpose

    CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Insert

    CasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    2A-eGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AbVec2.0-IGHG1

    Plasmid

    #80795

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hCathepsin L

    Plasmid

    #11250

    Purpose

    Mammalian expression of human cathepsin L

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Has Service

    Cloning Grade DNA

    Insert

    cathepsin L (CTSL Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP-E-Syt2

    Plasmid

    #66831

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt2 (ESYT2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-polyQ74-NLS

    Plasmid

    #231892

    Purpose

    Forms nuclear HTT partial exon 1 Q74-NLS aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge