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176,453 results

Showing: 2791 - 2820 of 176453 results

  1. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Expression
    Mammalian
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    177938-plasmid-map-sequence-id-429078

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  9. lentiGuide-Hygro-mTagBFP2

    Plasmid
    #99374
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17 Articles
    99374-plasmid-map-sequence-id-430620

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  17. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65767-plasmid-map-sequence-id-471631

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  22. pUC57 APEX

    Plasmid
    #40306
    Purpose
    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103
    Insert
    pea APEX (Synthetic)
    Use
    Unspecified
    Mutation
    K14D, W41F, E112K (relative to wild-type)
    Available since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    40306-plasmid-map-sequence-id-115990

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  27. pLenti-CaMKIIa-ChETA-EYFP

    Plasmid
    #26967
    Purpose
    3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Publication
    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi
    Insert
    hChR2(E123T/H134R)-EYFP
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E123T
    Promoter
    CaMKIIa
    Available since
    Jan. 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    26967-plasmid-map-sequence-id-12770

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Showing: 2791 - 2820 of 176453 results