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Addgene

We narrowed to 8,764 results for: Plat

Showing: 2821 - 2850 of 8764 results
  1. ITGB3

    Plasmid
    #51921
    Purpose
    Expresses full-length Integrin beta-3 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    ITGB3 (ITGB3 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 718, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. qTAG-C-Solo-mStayGold-COX8A

    Plasmid
    #227309
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)
    Depositor
    Insert
    COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. qTAG-C-Solo-mStayGold-LAMP1

    Plasmid
    #227322
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)
    Depositor
    Insert
    LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. FGG

    Plasmid
    #52031
    Purpose
    Expresses full-length Fibrinogen gamma chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    FGG (FGG Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 454, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. FGB

    Plasmid
    #52030
    Purpose
    Expresses full-length Fibrinogen beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    FGB (FGB Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 492, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. qTAG-C-mStayGold-Puro-CLTC

    Plasmid
    #227314
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)
    Depositor
    Insert
    CLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. qTAG-C-Solo-mStayGold-PEX3

    Plasmid
    #227304
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the PEX3 locus. For peroxisome visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PEX3 (Addgene #227303)
    Depositor
    Insert
    PEX3 Homology Arms flanking a mStayGold Tag (PEX3 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. qTAG-N-Solo-mStayGold-RAB7A

    Plasmid
    #227298
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the RAB7A locus. For endosome visualization. To be co-transfected with sgRNA plasmid px330-RAB7A (Addgene #227297)
    Depositor
    Insert
    RAB7A Homology Arms flanking a mStayGold Tag (RAB7A Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. qTAG-N-Solo-mStayGold-GOLGA2

    Plasmid
    #227325
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)
    Depositor
    Insert
    GOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. DAG1-bio-His

    Plasmid
    #51651
    Purpose
    Expresses full-length Dystroglycan precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    DAG1 (DAG1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. qTAG-N-Solo-mScarlet-LMNB1

    Plasmid
    #207771
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Insert
    LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. LTBP1-bio-His

    Plasmid
    #53421
    Purpose
    Expresses full-length Latent-transforming growth factor beta-binding protein 1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    LTBP1 (LTBP1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  13. BSG-bio-His

    Plasmid
    #53146
    Purpose
    Expresses full-length Basigin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    Basigin (BSG Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
  14. qTAG-C-mNeon-Puro-TOMM20

    Plasmid
    #207790
    Purpose
    Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Insert
    TOMM20 Homology Arms flanking a mNeon-Puro Cassette (TOMM20 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  15. CD154-His-bio

    Plasmid
    #52337
    Purpose
    Expresses full-length Tumor necrosis factor ligand superfamily member 5 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Insert
    CD154 (CD40LG Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  16. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid
    #170185
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.
    Depositor
    Inserts
    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and hU6
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. pODN-mNG-ANLN

    Plasmid
    #183834
    Purpose
    Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.
    Depositor
    Insert
    ANLN homology arms with mNeonGreen-linker (ANLN Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  18. qTAG-N-Blast-3xHA-LMNB1

    Plasmid
    #207778
    Purpose
    Donor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Insert
    LMNB1 Homology Arms flanking a Blast-3xHA Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  19. pLP-PIGA (pLP140)

    Plasmid
    #168461
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 2821 - 2850 of 8764 results