We narrowed to 9,389 results for: Coli

1. pET28a-HsSENP5

   Plasmid

   #71468

   Purpose

   Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 5 (SENP5 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pRSET FLIPE-10u

   Plasmid

   #13538

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207R
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   A207R in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSR26-2

   Plasmid

   #104335

   Purpose

   Expresses ompR from a TetR reuglated promoter under inducible ATC control.

   Depositor

   Jeffrey Tabor

   Article

   Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

   Insert

   OmpR (ompR Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLTetO1

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKDC.015

   Plasmid

   #184992

   Purpose

   Express -Eco2 RT and ncRNA in human cells

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco2: RT and ncRNA(wt), a1/a2 length: 13
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Human codon optimized RT

   Promoter

   TetOn-3g

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSET FLIPE-1m

   Plasmid

   #13540

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   A207W in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EC.SurA.TYB1.a172-274.wt

   Plasmid

   #15934

   Depositor

   David McKay

   Article

   Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

   Insert

   Peptidyl prolyl isomerase domain 1 of SurA (surA E. coli)
   

   Use

   Tags

   intein

   Expression

   Bacterial

   Mutation

   wild type

   Promoter

   Available since

   Nov. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPJ174(JPUB_006779)

   Plasmid

   #83827

   Purpose

   Carries operon 1 final

   Depositor

   Harry Beller

   Article

   Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

   Inserts

   Kuste3603
   kuste3605
   kuste3606
   

   Use

   Pfab217 (p15a ori, kanr)

   Tags

   Expression

   Mutation

   Promoter

   op1_3603_for, op1_3605_for, and op1_3606_for

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSET FLIPglu-600uDelta11 Aphrodite

   Plasmid

   #13569

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16A Delta11 Aphrodite
   

   Use

   Tags

   Aphrodite and ECFP

   Expression

   Bacterial

   Mutation

   Fluorophore-derived linker shortened. F16A mutati…

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFLIPglu-3.2mDelta13

   Plasmid

   #12992

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16A S112A Delta13
   

   Use

   Plant nanosensor

   Tags

   ECFP and EYFP

   Expression

   Plant

   Mutation

   F16A, S112A in binding protein

   Promoter

   Available since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pFLIPglu-170nDelta13

    Plasmid

    #12989

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16F Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    WT binding protein (no F16A)

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pKDsgRNA-ldhA

    Plasmid

    #102288

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    ldhA gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKDsgRNA-rpsL

    Plasmid

    #89953

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rpsL gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDTnLAPP2A frame 0

    Plasmid

    #28226

    Depositor

    Harald von Melchner

    Article

    Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

    Insert

    Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
    

    Use

    Retroviral

    Tags

    EGFP and nLAP-tag

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBP-pET-RBS

    Plasmid

    #72981

    Purpose

    PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    pET RBS derived from pET vectors
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HL 818 (=HL 716 + pHL 98)

    Plasmid

    #30006

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLlacO-1:DsrA-mcherry, pLtetO-1:rpoS::gfp
    

    Use

    Synthetic Biology

    Tags

    mcherry, GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pETM6_Ptac_BmHps_BmPhi_BsMdh

    Plasmid

    #129103

    Purpose

    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Whitaker et al Metab Eng. 2017 Jan;39:49-59. doi: 10.10

    Insert

    BsMDH BmHPS BmPHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pPB-DEST-R1R2-pNeo

    Plasmid

    #141057

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Neomycin resistance gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR-L1-PGK-Neo-L2

    Plasmid

    #141007

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Neomycin resistance gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JBEI-6823

    Plasmid

    #84986

    Purpose

    For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

    Depositor

    Taek Soon Lee

    Article

    George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

    Insert

    MevTaco-PMKco-Mkco
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHCKan-yibDp-mdlC

    Plasmid

    #134590

    Purpose

    Low Phosphate inducible mdlC benzylformate decarboxylase

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mdlC (benzylformate decarboxylase) C -terminal his tag
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDisplay FLIPE-100u

    Plasmid

    #13547

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    S118W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSET FLIPE-100u

    Plasmid

    #13539

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    S118W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)

    Plasmid

    #70210

    Purpose

    1 Part of 4-part switch

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNIC28-BSA4-G-S10

    Plasmid

    #127828

    Purpose

    Production of recombinant human ribosomal protein S10

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HL 3904 (=HL 770 + pHL 991)

    Plasmid

    #30093

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

26. DVA_AG

    Plasmid

    #66042

    Purpose

    MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:G]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSET FLIPglu-600uDelta13 Aphrodite

    Plasmid

    #13570

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta13 Aphrodite
    

    Use

    Tags

    Aphrodite and ECFP

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKDsgRNA-malG

    Plasmid

    #89951

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    malG gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Beta lactamase S68TAG

    Plasmid

    #68305

    Purpose

    Beta lactamase S68TAG gene

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    S68TAG beta lactamase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET3a pro-hRegIIIa

    Plasmid

    #64935

    Purpose

    bacterial expression of full length human RegIIIalpha

    Depositor

    Lora Hooper

    Article

    Cash et al Protein Expr Purif. 2006 Jul;48(1):151-9

    Insert

    RegIIIa (REG3A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations made in 5' end to relax secondary …

    Promoter

    T7

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pKDsgRNA-hisG

    Plasmid

    #89954

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    hisG gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTU2-A-RFP

    Plasmid

    #72954

    Purpose

    Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - A site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDTnLAPP2A frame 2

    Plasmid

    #28228

    Depositor

    Harald von Melchner

    Article

    Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

    Insert

    Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
    

    Use

    Retroviral

    Tags

    EGFP and nLAP-tag

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNH-TrxT-S15

    Plasmid

    #127822

    Purpose

    Production of recombinant human ribosomal protein S15

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS15 (RPS15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNH-TrxT-G-S15

    Plasmid

    #127823

    Purpose

    Production of recombinant human ribosomal protein S15

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS15 (RPS15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV Lex VP16 (F442A) HA (P#1710)

    Plasmid

    #14595

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Use

    Tags

    HA and VP16 (F442A)

    Expression

    Mammalian

    Mutation

    The VP16 sequence contains an F442A mutation. The…

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

37. pZS1[LacI-L]

    Plasmid

    #60745

    Purpose

    Contains PI driving expression dimeric LacI, the Lactose inducible repressor.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Insert

    LacI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    wt LacI without the tetramerization domain

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSL6 SMR1747

    Plasmid

    #28029

    Depositor

    Susan Rosenberg

    Article

    Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

    Insert

    MutS (mutS E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pRSET FLIPE intramol-1m

    Plasmid

    #13551

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIP-E intramol A207W (gltI E. coli)
    

    Use

    Tags

    EYFP

    Expression

    Bacterial

    Mutation

    A207W, ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. DVA_DH

    Plasmid

    #66049

    Purpose

    MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:H]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge