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We narrowed to 155,986 results for: ins

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Showing: 2851 - 2880 of 155986 results

  7. pAJE3-E7

    Plasmid
    #214359
    Purpose
    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins
    Depositor
    Ryan Mehl
    Article
    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-
    Inserts
    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    Expression
    Bacterial
    Promoter
    AmpR and lpp
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214359-plasmid-map-sequence-id-424910

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  8. pAED2

    Plasmid
    #234599
    Purpose
    Fluorescent reporter of the SOS response
    Depositor
    Tanel Tenson
    Article
    Kaldalu et al NPJ Antimicrob Resist. 2025 Apr 11;3(1):
    Inserts
    mScarlet-I
    gfp-mut2
    Use
    Reporter
    Expression
    Bacterial
    Mutation
    GFPmut2 was derived from avGFP with the following…
    Promoter
    Ptet+dnaK P1 and cda
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234599-plasmid-map-sequence-id-467741

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  9. MCS-3_Endofin_LgBiT

    Plasmid
    #182574
    Purpose
    This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.
    Depositor
    Bradley McConnell
    Article
    Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1
    Insert
    zinc finger FYVE-type (ZFYVE16 Human)
    Tags
    LgBiT (Large BiT)
    Expression
    Mammalian
    Mutation
    FYVE domain Q739-K806
    Promoter
    Herpes simplex virus (HSV)
    Available Since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    182574-plasmid-map-sequence-id-450120

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  11. G protein alpha-q-YFP

    Plasmid
    #55782
    Purpose
    This G protein alpha-q construct contains internal insertions of YFP and the EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    alpha-q EE YFP (Gnaq Mouse)
    Tags
    The EE epitope was introduced into the alpha-q se…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    55782-plasmid-map-sequence-id-119295

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  14. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid
    #139994
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139994-plasmid-map-sequence-id-275519

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  23. pAAV-hsynapsin-DIO-scramble shRNA - mCherry

    Plasmid
    #245064
    Purpose
    AAV transgene designes to express scramble (control) shRNA sequence cell-type selectively. Scramble hairpin expressed from the mir30 cassette together with mCherry.
    Depositor
    William Wisden
    Article
    Anuncibay Soto et al Proc Natl Acad Sci U S A. 2025 Oct 14;12
    Insert
    AAV-DIO-scramble-shRNA-mCherry
    Use
    AAV, Cre/Lox, and RNAi
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    human synapsin
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    245064-plasmid-map-sequence-id-489358

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  25. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid
    #22028
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22028-plasmid-map-sequence-id-213358

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  26. pKK-TEV-ProteinA

    Plasmid
    #105788
    Purpose
    Expression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-ProteinA
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105788-plasmid-map-sequence-id-203379

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  28. pPICZc-ScAct1*-thymosinB-8His

    Plasmid
    #111148
    Purpose
    Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    Act1 (ACT1 Budding Yeast)
    Use
    Pichia pastoris integration plasmid
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Promoter
    AOX1
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111148-plasmid-map-sequence-id-214055

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Showing: 2851 - 2880 of 155986 results