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We narrowed to 5,238 results for: codon optimized

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Showing: 4801 - 4850 of 5238 results

  2. CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid
    #171385
    Purpose
    Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Article
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Insert
    humanized minidCas13X.1-RESCUE-S
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171385-plasmid-map-sequence-id-337353

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  7. pSCAR_Cas9-hygro_GFP

    Plasmid
    #162075
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available Since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162075-plasmid-map-sequence-id-325304

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  8. pYLCRISPR/Cas9P35S-N

    Plasmid
    #66191
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66191-plasmid-map-sequence-id-404047

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  10. EFS-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222500
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    EFS
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222500-plasmid-map-sequence-id-441137

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  11. pDONR207 SARS-CoV-2 NSP3

    Plasmid
    #141257
    Purpose
    Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141257-plasmid-map-sequence-id-288044

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  13. pAB2076 pAAV REPAIR.t1

    Plasmid
    #176323
    Purpose
    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    Use
    AAV and CRISPR
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    EFS (short EF1alpha) and hU6
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176323-plasmid-map-sequence-id-345286

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  15. pYLCRISPR/Cas9P35S-B

    Plasmid
    #66190
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66190-plasmid-map-sequence-id-169495

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  19. pDONR207 SARS-CoV-2 E

    Plasmid
    #141273
    Purpose
    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141273-plasmid-map-sequence-id-288100

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  20. pDONR207 SARS-CoV-2 M

    Plasmid
    #141274
    Purpose
    Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141274-plasmid-map-sequence-id-288102

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  21. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

    Plasmid
    #197031
    Purpose
    Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized denOsCas12f1-VPR
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197031-plasmid-map-sequence-id-388973

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  22. pDONR223 SARS-CoV-2 S

    Plasmid
    #149329
    Purpose
    Gateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149329-plasmid-map-sequence-id-296492

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  24. pDONR223 SARS-CoV-2 N

    Plasmid
    #149330
    Purpose
    Gateway-compatible Entry vector, with insert of the nucleocapsid (N) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    N (N SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149330-plasmid-map-sequence-id-296491

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  33. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

    Plasmid
    #197028
    Purpose
    AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Use
    AAV
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197028-plasmid-map-sequence-id-388968

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  49. pYeDP60-hATP13A2-E343A

    Plasmid
    #171378
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Peter Vangheluwe
    Article
    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:
    Insert
    human ATP13A2 variant 2 wild-type
    Tags
    BAD tag
    Expression
    Yeast
    Mutation
    E343A
    Promoter
    URA3
    Available Since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171378-plasmid-map-sequence-id-342930

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Showing: 4801 - 4850 of 5238 results