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Plasmid#114193PurposeCreation of a stable cell line expressing APP mutation (AD in vitro study)DepositorInsertAmyloid-Precursor-Protein (APP Human)
ExpressionMammalianMutationwild type splice variant 695: 695AA (2085bp, lack…PromoterCMVAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dCas9-CBP FL
Plasmid#179550Purposeencodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-WD40
Plasmid#25073DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLRRK2 (LRRK2 Human)
Tags2XMycExpressionMammalianMutationContains only the WD40 domain aa 2155-2527Available sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0037
Plasmid#181934PurposeDual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsGaussia luciferase
Cypridina luciferase
ExpressionMammalianPromoterCMV and EF1alphaAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAJM.847
Plasmid#108524PurposePhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.DepositorInsertPhlFAM + PPhlF-YFP
ExpressionBacterialMutationPhlF: S50G S73I R95H A121V M137I N144D T155I R200CAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFS461
Plasmid#89064Purposeleu1 integration plasmid with adh1pr:Z3EVDepositorInsertZ3EV artificial transcription factor
UseLeu1 integrating vectorPromoteradh1Available sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pScarlessHD-DsRed-w+
Plasmid#80801PurposeVector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for identifying backbone incorporation.DepositorInsertspiggyBac-3xP3-DsRed
GMR-white
UseHigh copy, amp resistancePromoter3xP3 and GMR::hsp70Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dCas9-CBP core
Plasmid#179543Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertS. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
2XMyc-LRRK2-RCKW
Plasmid#25064DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLRRK2 (LRRK2 Human)
Tags2XMycExpressionMammalianMutationContains only the ROC-COR-Kinase-WD40 domains …Available sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)
Plasmid#197511PurposeCbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacerDepositorInsertAAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
UseAAV and CRISPRTagsBPNLS and BPNLS-NpuC(intein)MutationC-terminal mutations of SpRY(L1111R/D1135L/S1136W…PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-rABE-Flag
Plasmid#191383PurposeDoxycycline inducible expression of RNA A-to-I editor rABEDepositorInsertrABE-Flag
UseLentiviral and Synthetic BiologyTagsFlagExpressionMammalianPromotertet-inducible promoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-uvsY
Plasmid#163914PurposeExpresses uvsY for bacterial expression and affinity purificationDepositorAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
opto-RhoA-mCherry_pcDNA3.1
Plasmid#164472PurposeExpresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertopto-RhoA
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CRY2high-mCherry
Plasmid#104063PurposeExpresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRY2high-mCherry
ExpressionMammalianMutationCRY2PHR E490RPromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PGK plasmid
Plasmid#169744PurposeUnregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyExpressionMammalianPromoterPGK promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-Ubiquitin-K6R
Plasmid#121153PurposeMammalian expression of HA tagged ubiquitin K6RDepositorAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-ARP2-C-14
Plasmid#54979PurposeLocalization: Actin Related Protein, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertARP2 (ACTR2 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEMS-II-PEST
Plasmid#204766PurposeGEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-PEST-m6Alinker-DHFR
Mutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMTL3
Plasmid#127966Purposeconstitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAAVS1-CAG-KRAB-dCas9-BFP-KRAB
UseTALENExpressionMammalianPromoterCAGAvailable sinceNov. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI H2B-RFP
Plasmid#92398PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIRES H2B RFP
ExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by