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Plasmid#189851PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP**-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP** (modified T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-cat-P-R
Plasmid#189853PurposeCre-inducible activation of cat for chloramphenicol resistance.DepositorInsertP-loxP-TT-loxP-R-cat
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
dTAG-IRF8
Plasmid#190620PurposeLentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradationDepositorInsertIRF8 (IRF8 Human)
UseLentiviralTagsFKBP12_G36VExpressionMammalianMutationsynonymous mutation for sgRNA resistance for sgIR…PromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pPPC009.106
Plasmid#171142PurposeExpression of J106 scRNA on pBBR1-KmR plasmidDepositorInsertJ106 scRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCF806-shABC1
Plasmid#186713PurposeExpresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.DepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B ActR-IB
Plasmid#14050DepositorInsertActR-IB (ACVR1B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NR
Plasmid#89625PurposeExpresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG inductionDepositorInsertNitrate reductase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
mApple-PCNA-19-NLS-4
Plasmid#54937PurposeLocalization: Nucleus, Excitation: 568, Emission: 592DepositorInsertPCNA-19 NLS (PCNA Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PfICH pET15b
Plasmid#60684PurposeRecombinant protein expression of Isocyanide Hydratase from Pseudomonas fluorescensDepositorInsertinhA
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PS-CFP2-TBRII-N-18
Plasmid#57249PurposeLocalization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 / 511DepositorInsertTBRII (TGFBR2 Human)
UseTagsPS-CFP2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMIIS928
Plasmid#238211PurposeExpresses TaTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.DepositorInsertTaTasR
UseTagsHA-NLSExpressionMammalianMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV.tetO.Ascl1.U6.shRest1.U6.shRest2
Plasmid#234846Purpose3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promotersDepositorInsertAscl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
eGFP-MYO5B
Plasmid#239265PurposeExpression of N terminal GFP tagged Myo5BDepositorInsertMYO5B (MYO5B Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMIIS948-CXCR4
Plasmid#238213PurposeTasR tigRNA expression to target CXCR4 under U6 promoter in human cells.DepositorInserttigRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-EGFP-PA-BAX(ER)
Plasmid#228941PurposeStable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.DepositorUseRetroviralTagsEGFPExpressionMammalianMutationN-terminus and C-terminus are deleted. BAX(3-171)…PromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-EGFP-LOV2-Cyb5a
Plasmid#233348PurposeStable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.DepositorInsertsUseRetroviralTagsEGFPExpressionMammalianMutationN-terminus is deleted (Cyb5a 95-134) and N538EPromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-SEC61B-mCherry-FRB
Plasmid#233349PurposeStable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.DepositorInsertsUseRetroviralTagsExpressionMammalianMutationT2098L mutant of FKBP-rapamycin binding domain of…PromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-FKBP-HaloTag
Plasmid#233350PurposeStable expression of FKBP-Halotag in mammalian cells. FKBP-halotag localizes in the cytosol and binds to the FRB tag in the presence of AP21967 (A/C Heterodimerizer).DepositorInsertFKBP
UseRetroviralTagsHaloTag7ExpressionMammalianMutationPromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-TetOn-mCherry-OAZ1_FS-YFP
Plasmid#232356PurposeMammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)DepositorInsertOAZ1 derived polyamine sensing module (OAZ1 Human)
UseLentiviralTagseYFP and mCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3KB5
Plasmid#234225PurposeHeterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coliDepositorInsert3KB5 (TRIM72 Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRExT2A-NT-PmNG
Plasmid#232200PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDB_270
Plasmid#231111PurposeC>T base editorDepositorInsertTadA-CDd-nCas9-SpG-UGI-HA
UseCRISPR and LentiviralTagsExpressionMutationD10APromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA_867
Plasmid#231109PurposeA>G base editorDepositorInsertABE8e-nCas9-SpG-HA
UseCRISPR and LentiviralTagsExpressionMutationD10APromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sasgRNACMV- mCherry-WPREpA
Plasmid#217015PurposeS. aureus sgRNAs backbone along with mCherry expressionDepositorInsertmCherry
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMVmin-BC0538-luc2
Plasmid#227140PurposeBarcoded assay, Ca2+ sensor; barcode BC05038DepositorInsert6x clustered NFAT element linked to CMV minimal promoter driving barcode 05038 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro-H2BC11
Plasmid#227332PurposeDonor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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AfNitB_pET29b
Plasmid#215401PurposeExpression construct for AfNitB gene, codon optimized for expression in E. coliDepositorInsertAfNitB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MEK1-E102-I103del-V5
Plasmid#217301PurposeLentivirus vector for expression of MEK1 E102-I103deletionDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsV5ExpressionMutationE102-I103deletionPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
EXT1- D164A-pLJC2-3XFLAG
Plasmid#215828PurposeEctopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cellsDepositorInsertExostosin glycosyltransferase 1 Mutant (EXT1 Human)
UseLentiviralTags3X FlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W
Plasmid#211969PurposeExpress gRNA against LIN28A with puro and BFPDepositorInsertsgRNA targeting LIN28A (LIN28A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W
Plasmid#211968PurposeExpress gRNA against LIN28A with puro and BFPDepositorInsertsgRNA targeting LIN28A (LIN28A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
p194-RV-CAG-dio-mScarlet-T2A-Brn2
Plasmid#204741PurposeConditional expression of target gene Brn2 with fluorescent reporter mScarletDepositorInsertBrn2
UseRetroviralTagsExpressionMutationPromoterCAGAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPPC005
Plasmid#171140PurposeFor integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertXylS-Pm
UseCRISPRTagsExpressionBacterialMutationPromoterPmAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT121_GalL_nCDA1-VQRBE3
Plasmid#145086PurposeExpressing base editor nCDA1-VQRBE3 in yeast cellsDepositorInsertnCDA1-VQRBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, D1135V, R1335Q, T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-D769H
Plasmid#116327PurposeLentiviral expression of ERBB2 D769HDepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationD769HPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN51 (R40A)
Plasmid#80109PurposeLate1-O3-33-myc-PH(R40A)DepositorInsertEPN-51 (R40A)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV Marina-T2A-nls-mCherry
Plasmid#74216Purposegenetically-encoded fluorescent voltage indicatorDepositorInsertfluorescent protein voltage sensor
UseTagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only