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Showing: 4851 - 4900 of 13002 results

  9. pBbB8k-csg-NbSARS

    Plasmid
    #171703
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA is fused to a cross-reactive nanobody targeting the spike protein of betacoronaviruses via a flexible linker.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1
    Insert
    csgBACEFG, with nanobody fused to CsgA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171703-plasmid-map-sequence-id-336471

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  11. pCAG-EGxxFP-CD55

    Plasmid
    #153959
    Purpose
    Used for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequences
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    CD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
    Use
    A plasmid specific for egxxfp assay
    Tags
    N/A
    Expression
    Mammalian
    Mutation
    No mutation
    Promoter
    CAG promoter
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    153959-plasmid-map-sequence-id-319276

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  28. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

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  29. pcDNA3/er-(n2)oxStayGold(c4)v2.0

    Plasmid
    #186296
    Purpose
    A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.
    Depositor
    Atsushi Miyawaki
    Article
    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103
    Insert
    er-(n2)oxStayGold(c4)v2.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186296-plasmid-map-sequence-id-366145

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  30. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

    Plasmid
    #121966
    Purpose
    Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.
    Depositor
    Clifford Brangwynne
    Article
    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.
    Insert
    scFv-GCN4
    Use
    Lentiviral
    Tags
    superfold GFP-GB1-NLS-iLID
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    121966-plasmid-map-sequence-id-236121

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  42. pMXs-IP- SCAT3.2

    Plasmid
    #172497
    Purpose
    SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    Amcyan
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    MLV LTR
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172497-plasmid-map-sequence-id-340999

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  50. pCherryGFP-CoV2-Inframe-stop

    Plasmid
    #177620
    Purpose
    Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).
    Depositor
    Junjie Guo
    Article
    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11
    Insert
    SARS-CoV-2 FSE
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177620-plasmid-map-sequence-id-350046

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Showing: 4851 - 4900 of 13002 results