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We narrowed to 12,157 results for: pcDNA

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Showing: 4851 - 4900 of 12157 results

  5. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

    Plasmid
    #192445
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available Since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192445-plasmid-map-sequence-id-379137

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  7. pcDNA3-mCherry-TSC2(AA)-NLS

    Plasmid
    #192443
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192443-plasmid-map-sequence-id-379076

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  13. pcDNA-zeo-hFJX1-myc

    Plasmid
    #46930
    Purpose
    expression of human FJX1-myc in mammalian cells
    Depositor
    R.D. Beauchamp, Natasha Deane
    Insert
    FJX1 (FJX1 Human)
    Tags
    myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H

    Plasmid
    #108375
    Purpose
    FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    108375-plasmid-map-sequence-id-209429

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  20. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid
    #206114
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Expression
    Mammalian
    Mutation
    E1658A
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206114-plasmid-map-sequence-id-415366

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  29. pcDNA3-HCMVgO-8his (Merlin)

    Plasmid
    #136454
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tag
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein O (UL74 Human betaherpesvirus 5)
    Tags
    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136454-plasmid-map-sequence-id-269878

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  32. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid
    #114143
    Purpose
    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    Expression
    Mammalian
    Available Since
    Oct. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    114143-plasmid-map-sequence-id-224089

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  42. GFP Cav2.2 HA D665A pcDNA3

    Plasmid
    #206113
    Purpose
    expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional
    Depositor
    Annette Dolphin
    Insert
    cacna1b (CACNA1B Rabbit)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    D665A
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206113-plasmid-map-sequence-id-415392

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  43. cacna2d1 R241A D491A HA pcDNA3

    Plasmid
    #206109
    Purpose
    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding
    Depositor
    Annette Dolphin
    Insert
    cacna2d1 (Cacna2d1 Rat)
    Expression
    Mammalian
    Mutation
    R241A D491A
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206109-plasmid-map-sequence-id-415382

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Showing: 4851 - 4900 of 12157 results