We narrowed to 3,637 results for: mpo

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p3XFLAG-TAK1-K63A

Plasmid

#43933

Depositor

Bryant Darnay

Article

Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

Insert

Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)

Tags

3XFLAG

Expression

Mammalian

Mutation

K63A

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAL204

Plasmid

#22282

Purpose

Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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ZIKV_Sensor_27B_LacZ

Plasmid

#75006

Purpose

Toehold switch sensor to detect Zika Virus

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_sensor_27B_LacZ

Tags

LacZ

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

Plasmid

#86194

Purpose

beta Globin NMD construct with and without PTC under Bi-directional TET on promoter

Depositor

Robert Singer

Article

Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

Insert

beta Globin (HBB, Hbb Human, Mouse)

Use

Tet on

Expression

Mammalian

Mutation

39TER in beta Globin in MCS II

Promoter

Tet on

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSR358.4

Plasmid

#125114

Purpose

Expression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-liaR(DBD)159
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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PA-Dre

Plasmid

#169706

Purpose

Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

Dre246-nMag-T2A-pMag-Dre247

Use

AAV

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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Cre-responsive Cas9 expressing transposon library vector

Plasmid

#164138

Purpose

Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Synthetic Biology

Expression

Mammalian

Promoter

human U6, EF-1a

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBX_RPL7a_smRuby2_HA

Plasmid

#236087

Purpose

Constitutively encodes RPL7a-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting the 60S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPL7a-smRuby2-HA (RPL7A Human, Synthetic)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-PermE

Plasmid

#209446

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Promoter

PtipA; sgRNA expression from ermE* promoter

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Ubc13-C87A

Plasmid

#43939

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

ubiquitin-conjugating enzyme E2N (UBE2N Human)

Tags

2x FLAG

Expression

Mammalian

Mutation

C87A

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT2h

Plasmid

#208721

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2h

Use

AAV

Promoter

hSyn

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Plasmid

#182063

Purpose

Co-expression of Arch-based voltage indicator (QuasAr6a) and membrane-localized calcium reporter (jRGECO1a-CAAX)

Depositor

Adam Cohen

Article

Landau et al eLife. 2022 Mar 23;11. pii: 76993. doi:

Insert

QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Use

Lentiviral

Promoter

CAG

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1

Plasmid

#138419

Purpose

CAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Insert

dCas9

Use

Lentiviral

Tags

BioTAP-tag

Promoter

EF1a

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-FLJ20436

Plasmid

#29757

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

FLJ20436 (C12orf41 Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only
pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster nanos

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU

Plasmid

#31726

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

*

Promoter

None

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pMGIB-3xFLAG-pontin

Plasmid

#53608

Purpose

Retroviral expression of human pontin

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

pontin (RUVBL1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

pontin CDS mutated to confer resistance to shRNA.…

Promoter

LTR

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR

Plasmid

#174883

Purpose

Targeting vector for generating Pou5f1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Pou5f1

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP C1

Plasmid

#202424

Purpose

Encodes GFP alone

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(T31N)-HA

Plasmid

#79429

Purpose

Expresses C-terminally HA-tagged Arf5(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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