We narrowed to 31,526 results for: Eng
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Plasmid#219436PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInserttwo transposon ends, LoxP-terminator-LoxP gene cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pc3xTC20-alphaTubulin
Plasmid#36327DepositorInsertalpha tubulin 1b with optimized tetracysteine peptides for ReAsH labeling (TUBA1B Synthetic, Human)
UseTags3xTC20ExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1 Sesn2
Plasmid#61873Purposebacterial expression of a GST-Sesn2 (mouse)DepositorInsertSesn2 (Sesn2 Mouse)
UseTagsGSTExpressionBacterialMutationnonePromoterAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG9 stopGFP
Plasmid#114669PurposeExpresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tagDepositorInsertATG9
UseTagsGFPExpressionYeastMutationChanged the three linker base pairs after the las…PromoterATG9Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
2a-copGFP(+HA)
Plasmid#83806PurposeExpress copGFP reporter for HDR-induced knock-inDepositorInsertcopGFP
UseHomologous recombination for gapdh lociTagsExpressionMutationPromoterAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-UAS-Dendra2
Plasmid#97145PurposeFor Gal4/UAS expression systemDepositorInsertDendra2
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L3-NF-L1-dMb2Cas12a
Plasmid#177686PurposeGateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editingDepositorInsert2xFokI-dMb2Cas12a
UseCRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
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PECAM-H2B-GFP
Plasmid#14689DepositorInsertPECAM promoter/enhancer (Pecam1 Mouse)
UseTagsH2B-GFPExpressionMammalianMutationPromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
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Ef1a_NANOG_Ires_Puro
Plasmid#18920DepositorInsertNANOG (NANOG Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c
Plasmid#107599PurposeAn entry vector with U6a and U6c promoter driving guide RNAs expressionDepositorInsertsU6a promoter
U6c promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrex-b-NLS-hSpCas9
Plasmid#62543PurposeExpression of Cas9 in T. cruziDepositorInsertNLS-hSpCas9
UseCRISPR; Trypanosoma cruzi expressionTags3xFLAG and NLSExpressionMutationPromoterRibo-HX1Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF.mHES1.Ubc.GFP
Plasmid#17625DepositorInsertHES1 (Hes1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSM_ser-2prom3p::myr-mCherry
Plasmid#164223PurposeExpression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.DepositorInsertmyr-mCherry
UseTagsExpressionWormMutationPromoterser-2promo3Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
plenti-arRNA-BFP-backbone
Plasmid#180221PurposeFor the cloning of arRNA-expressing vectors containing BFP fluorescence marker in mammalian cellsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Oct4-ires-EGFP(lox neo)
Plasmid#21547DepositorInsertEGFP, Oct4
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
3539 pMIG Bax
Plasmid#8788DepositorInsertBax (Bax Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-Tab1-3'UTR
Plasmid#97155PurposeFor dual luciferase report assayDepositorInsertTab1 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PIGAZFN-L1
Plasmid#37797DepositorInsertPIGA zinc finger L1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR2B-dCas9-KRAB
Plasmid#115547PurposeEntry cassette for dCas9-KRABDepositorInsertdCas9-KRAB
UseTagsHAExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
PIGAZFN-R1
Plasmid#37799DepositorInsertPIGA zinc finger R1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L1-dMb2Cas12a
Plasmid#177683PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editingDepositorInsertFokI-dMb2Cas12a
UseCRISPR; Gateway compatible foki-dmb2cas12a entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS AluYa5rescueA70D
Plasmid#78657PurposeGenerates Alu inserts suitable for rescueDepositorInsertYa5rescue cassette
UseTagsExpressionMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTK_BsmBI_LacZ_Citrine_Nanotag_4
Plasmid#130514PurposeEnhancer cloning nanotag reporter vectorDepositorInsertLacZ
UseEnhancer cloning nanotag reporter vectorTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_BsmBI_LacZ_Cherry_Nanotag_105
Plasmid#130546PurposeEnhancer cloning nanotag reporter vectorDepositorInsertLacZ
UseEnhancer cloning nanotag reporter vectorTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
miR-vector-RFP pCDNA
Plasmid#97143Purposefor expression of miRNA and TagRFPDepositorTypeEmpty backboneUseTagsTagRFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC2xTC1-alphaTubulin
Plasmid#36326DepositorInsertalpha tubulin 1b with tetracysteine peptides for ReAsH labeling (TUBA1B Synthetic, Human)
UseTags2xTC1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds
Plasmid#119059PurposeFluorescent vector with zebrafish pax3a enhancer 5 (strong activity)DepositorInsertpax3a_enh5-E1b:eGFP
UseTagsExpressionBacterialMutationPromoterE1b minimal promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_BsmBI_LacZ_Cerulean_Nanotag_60
Plasmid#130528PurposeEnhancer cloning nanotag reporter vectorDepositorInsertLacZ
UseEnhancer cloning nanotag reporter vectorTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tt-NylC_pET28a
Plasmid#215431PurposeExpression construct for Tt-NylC gene, codon optimized for expression in E. coliDepositorInsertTt-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA8CHmneo
Plasmid#69608PurposeExpresses a neomycin tagged codon optimized L1PA8DepositorInsertL1PA8
UseTagsmneo retrotransposition cassetteExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK_BsmBI_LacZ_Cherry_Nanotag_117
Plasmid#130552PurposeEnhancer cloning nanotag reporter vectorDepositorInsertLacZ
UseEnhancer cloning nanotag reporter vectorTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMZ1001
Plasmid#204398PurposeA pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRpa0002Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
cPE
Plasmid#183549PurposeExpresses compact Prime Editor in mammalian cellsDepositorInsertSpCas9 nickase (H840A), Reverse Transcriptase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
PECAM-H2B-GFP-Hygro
Plasmid#14684DepositorInsertPECAM promoter/enhancer (Pecam1 Mouse)
UseTagsH2B-GFP and PGK-HygroExpressionMammalianMutationPromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIK-GW
Plasmid#126556PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatibleDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_BsmBI_LacZ_Cerulean_Nanotag_91
Plasmid#130539PurposeEnhancer cloning nanotag reporter vectorDepositorInsertLacZ
UseEnhancer cloning nanotag reporter vectorTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Citrine_Nanotag_23_neg_Control
Plasmid#130562PurposeEnhancer cloning nanotag reporter vectorDepositorInsertnegative oligo sequence
UseEnhancer cloning nanotag reporter vectorTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
hsa-miR-100 plix4.03
Plasmid#121128Purposeinducible expression in cellsDepositorInserthsa-miR-100 (MIR100 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA8WTmneo
Plasmid#69609PurposeExpresses a neomycin tagged wildtype L1PA8DepositorInsertL1PA8
UseTagsmneo retrotransposition cassetteExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2_B-act_dCas9-Vp64-T2A-Citrine
Plasmid#200248PurposeZebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesisDepositorInsertdCas9-Vp64-T2A-Citrine
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ4
Plasmid#83559PurposeUsed to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.DepositorInsertGAL7 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL7Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pTK_Citrine_Nanotag_5_neg_Control
Plasmid#130558PurposeEnhancer cloning nanotag reporter vectorDepositorInsertnegative oligo sequence
UseEnhancer cloning nanotag reporter vectorTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS B2rescue A70D
Plasmid#78658PurposeGenerates B2 inserts suitable for rescueDepositorInsertB2rescue cassette
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA4CHmneo
Plasmid#69607PurposeExpresses a neomycin tagged codon optimized L1PA4DepositorInsertL1PA4
UseTagsmneo retrotransposition cassetteExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only