We narrowed to 5,620 results for: SUP

1. pCF.111

   Plasmid

   #227187

   Purpose

   pORTMAGE-Ec1 + supplement E. coli SSB

   Depositor

   Seth Shipman

   Article

   Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

   Insert

   CspRecT, mutL_E32K, E. coli SSB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutL E32K

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLei-sfGFP-D134*

   Plasmid

   #191110

   Purpose

   An E. coli sfGFP reporter with one TAG at position 134

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   superfolder green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changing D134 to TAG

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLei-sfGFP-V2*D134*

   Plasmid

   #191111

   Purpose

   An E. coli sfGFP reporter with two TAG at positions 2 & 134

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   superfolder green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changing V2 & D134 to TAG

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-iGlu3slow1

   Plasmid

   #226501

   Purpose

   Superslow-decay iGluSnFR3 variant 1 for population imaging

   Depositor

   Katalin Torok

   Article

   Tran et al bioRxiv, August 30th, 2024

   Insert

   iGluSnFR3 D25A
   

   Use

   Tags

   IgK, Myc tag, and PDGFR Beta TM domain

   Expression

   Mammalian

   Mutation

   D25A

   Promoter

   CMV

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-iGlu3slow2

   Plasmid

   #226502

   Purpose

   Superslow-decay iGluSnFR3 variant 2 for population imaging

   Depositor

   Katalin Torok

   Article

   Tran et al bioRxiv, August 30th, 2024

   Insert

   iGluSnFR3 D25R
   

   Use

   Tags

   IgK, Myc tag, and PDGFR Beta TM domain

   Expression

   Mammalian

   Mutation

   D25R

   Promoter

   CMV

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPSt

   Plasmid

   #203590

   Purpose

   Base plasmid for Plant STARR-seq terminator libraries.

   Depositor

   Stan Fields

   Article

   Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSliceRep_A_miR-196a.M3

   Plasmid

   #220467

   Purpose

   Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Superfolder GFP
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSliceRep_A_miR-7

   Plasmid

   #220464

   Purpose

   Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Superfolder GFP
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSliceRep_A_miR-7.M4

   Plasmid

   #220465

   Purpose

   Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Superfolder GFP
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSliceRep_A_miR-196a

    Plasmid

    #220466

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GB1203

    Plasmid

    #215369

    Purpose

    TU for the expression of the silencing suppressor P19 driven by the 35s promoter

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    35s:P19:tNos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-msEGFP1.8

    Plasmid

    #208619

    Purpose

    Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.

    Depositor

    Quanfu Ma

    Insert

    msEGFP1.8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    (compared with pEGFP-C1, protein level) S30R, Y39…

    Promoter

    CMV

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. UBE2G2 shRNA

    Plasmid

    #201813

    Purpose

    siRNA

    Depositor

    Allan Weissman

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Jan 10. 1

    Insert

    UBE2G2 (UBE2G2 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDA313

    Plasmid

    #195214

    Purpose

    Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

    Insert

    sfGFP with tetO and a hairpin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET30-T7-lacO-His-sfGFP

    Plasmid

    #180754

    Purpose

    pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    G140C- Please see depositor comments

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET30-T7-lacO-sfGFP

    Plasmid

    #180756

    Purpose

    pET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DT039A

    Plasmid

    #159400

    Purpose

    Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23117-sfGFP
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    J23117 (weak) promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DT038A

    Plasmid

    #159399

    Purpose

    Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23106 (medium) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23106-sfGFP
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    J23106 (medium) promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1026L=U6-3-gRNA#1[Pol-γ35]

    Plasmid

    #159674

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#1[Pol-γ35]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1026M=U6-3-gRNA#2[Pol-γ35]

    Plasmid

    #159675

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#2[Pol-γ35]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. DT040A

    Plasmid

    #159401

    Purpose

    Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23117/J23106-sfGFP
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    hybrid J23117-J23106 promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

    Plasmid

    #162814

    Purpose

    STELLA plasmid for amber suppression of C-terminally labelled SMIM38

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    SMIM38(C-TAG)-Intein-DD
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    SMIM38(Ochre52,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAS1_4x7SKPylT_EF1_Ub-*SMIM38

    Plasmid

    #162808

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled SMIM38

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)SMIM38
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    SMIM38 (Met1, Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAS_4x7SKPylT_EF1_Ub-*PIGBOS

    Plasmid

    #162809

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)PIGBOS
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    PIGBOS (Met1, Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E

    Plasmid

    #125797

    Purpose

    Histone H2B-superfolder GFP and tdTomato fused by unskippable linker

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    H2BsfGFP-tdTomato
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUPD-SOD2MTS (12)

    Plasmid

    #101692

    Purpose

    SOD2MTS in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    CCAT-[MTS from S. cerevisiae Superoxide dismutase [Mn] (YHR008C)]-AATG
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO.1P shNFS1_4

    Plasmid

    #102966

    Purpose

    Suppression of NFS1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shNFS1_4 (NFS1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO.1P shNFS1_3

    Plasmid

    #102965

    Purpose

    Suppression of NFS1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shNFS1_3 (NFS1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CC1-Pos6

    Plasmid

    #101656

    Purpose

    CC1-Pos6 is a modified version of CC1-M240 that has 4 arginines in its linker. (* super ecliptic pHluorin A227D)

    Depositor

    Bradley Baker

    Article

    Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

    Insert

    CC1-Pos6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CC1-M240

    Plasmid

    #101657

    Purpose

    CC1 with shorter linker length and the 'GDP' fusion site. (* super ecliptic pHluorin A227D)

    Depositor

    Bradley Baker

    Article

    Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

    Insert

    CC1-M240
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR221-10X-tRNA

    Plasmid

    #70123

    Purpose

    Gateway DONR vector with cassette for expression of 10 intronless S. cerevisiae tRNAs

    Depositor

    Jay Hesselberth

    Article

    Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

    Insert

    10X-tRNA block
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None – SUP4 pol III promoter provided by insert

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

32. pVHed07

    Plasmid

    #82350

    Purpose

    Bxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmid

    Depositor

    Richard Murray

    Article

    Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

    Inserts

    mKate2 RFP
    Superfolder GFP
    Temporal logic gate with TP901 and Bxb1 attachment sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PP152

    Plasmid

    #78986

    Purpose

    Supplemental Figure 4. Promoter long ppTEF1. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PP153

    Plasmid

    #78997

    Purpose

    Supplemental Figure 4. Promoter GAP7. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PP87

    Plasmid

    #78987

    Purpose

    Supplemental Figure 4. Promoter scTEF1. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJBL-RNAINS4

    Plasmid

    #69456

    Purpose

    RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-IN S4 + SFGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    mutations for S4 variant (from wt)

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJBL-RNAINS3

    Plasmid

    #69455

    Purpose

    RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-IN S3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    mutations for S3 variant (from wt)

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LI D-E 11GFP

    Plasmid

    #54430

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Insert

    FLAG tag + and short linker + superfolder GFP 11 domain
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pQTEV-SUHW4

    Plasmid

    #34807

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    suppressor of hairy wing homolog 4 (Drosophila) (ZNF280D Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a T7pCONS TIR-1 sfGFP

    Plasmid

    #154463

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET28a T7pCONS TIR-2 sfGFP

    Plasmid

    #154464

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid

    #50474

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCI pHluorin Syt12

    Plasmid

    #184513

    Purpose

    Mammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 12 (Syt12 Rat)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV-CaMKIIa-hM4D(Gi)-mCherry

    Plasmid

    #50477

    Purpose

    Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Has Service

    AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM4D(Gi)-mCherry (CHRM4 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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45. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid

    #50454

    Purpose

    Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBI-EGFP-MnSOD

    Plasmid

    #16612

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    MnSOD (SOD2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMuLE_ENTR_TOP-iRFP_L5-L4

    Plasmid

    #113866

    Purpose

    Single pathway reporter; wnt promoter-driven expression of iRFP; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    TOP (wnt dependent promoter)
    iRFP713
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET-11b_PcrA_M6_H93A

    Plasmid

    #194156

    Purpose

    Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

    Depositor

    Taekjip Ha

    Article

    Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

    Insert

    Engineered helicase PcrA_M6
    

    Use

    Tags

    6x His tag and FLAG tag

    Expression

    Bacterial

    Mutation

    H93A, C96A, N187C, C247A, L384V, L409C

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. PTEN shRNA

    Plasmid

    #86645

    Purpose

    expression of shRNA targeting PTEN

    Depositor

    Jaime Modiano

    Article

    Lin et al Mol Cancer. 2007 Feb 14;6:16.

    Insert

    PTEN (PTEN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCI-SEP_NR2B

    Plasmid

    #23998

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    NR2B (Grin2b Rat)
    

    Use

    Tags

    NR2B signal sequence (aa1-31) and SEP (supereclip…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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