We narrowed to 44,084 results for: cha

1. pBbA5c-MevT-MBIS

   Plasmid

   #35150

   Depositor

   Jay Keasling, Taek Soon Lee

   Article

   Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

   Insert

   AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LacUV5,

   Available Since

   March 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRG713_lexO-HO_LexA-TF_URA3MX

   Plasmid

   #154821

   Purpose

   Inducible expression of HO endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   HO endonuclease (HO Budding Yeast)
   LexA-ER-B112
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NET47 pEGFP-N2 (593)

   Plasmid

   #61986

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET47 (TM7SF2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pVDM1001

   Plasmid

   #134660

   Purpose

   CRISPR delivery and repair template delivery vector

   Depositor

   Willem van Schaik

   Article

   de Maat et al FEMS Microbiol Lett. 2019 Nov 1;366(22).

   Insert

   CRISPR guide RNA array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFUGW-hGtACR1-EYFP

   Plasmid

   #67795

   Purpose

   Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

   Depositor

   John Spudich

   Article

   Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

   Insert

   hGtACR1
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVB3011

   Plasmid

   #113389

   Purpose

   Galactose-inducible L-BC gag: cure killer

   Depositor

   Suzanne Sandmeyer

   Article

   Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

   Insert

   L-BC Gag
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Please see depositor comments

   Promoter

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MXS_CMV::ZeoR-bGHpA

   Plasmid

   #62441

   Purpose

   MXS_chaining vector with CMV::ZeoR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Zeocin with CMV Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSF-CjPglB-LpxE

   Plasmid

   #128389

   Purpose

   Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Inserts

   PglB oligosaccharylktransferase
   LpxE phosphatase
   

   Use

   Tags

   Flag tag

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and pBAD

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFAP-Cre

   Plasmid

   #24704

   Depositor

   Michael Sofroniew

   Article

   Bush et al Neuron. 1999 Jun . 23(2):297-308.

   Insert

   Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP (glial fibrillary acidic protein)

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-VEGFR2-780

    Plasmid

    #21307

    Depositor

    Donald Ingber

    Article

    Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

    Insert

    VEGFR2 promoter (KDR Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP(C3)-Nup153-N

    Plasmid

    #64318

    Purpose

    GFP-tagged mammalian expression of N-terminal domain of Nup153

    Depositor

    Birthe Fahrenkrog

    Article

    Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

    Insert

    Nup153 (NUP153 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N-terminal domain

    Promoter

    CMV

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

12. NylCA_pET28a

    Plasmid

    #215418

    Purpose

    Expression construct for NylCA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NylCp2_pET28a

    Plasmid

    #215420

    Purpose

    Expression construct for NylCp2 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCp2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. L1_UBE2:mS-Lt-Ck3

    Plasmid

    #136131

    Purpose

    L1 in position 3, mark cell outline with mScarletI

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:mScarletI-Lti6b_3tNos-35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. AfNitB_pET29b

    Plasmid

    #215401

    Purpose

    Expression construct for AfNitB gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    AfNitB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP

    Plasmid

    #200277

    Purpose

    Characterisation of the sialic acid-responsive circuit with fluorescent reporter

    Depositor

    Matthew Chang

    Article

    Koh et al Nat Commun. 2022 Jul 4;13(1):3834. doi:

    Inserts

    CadC
    NanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAUC40

    Plasmid

    #71298

    Purpose

    Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

    Depositor

    Susanne von Bodman

    Article

    Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mOtop1_pcDNA3

    Plasmid

    #114677

    Purpose

    This construct was used to express mouse Otop1 in HEK-293 cells

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 1 (Otop1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SNP (rs49325632): Glycine 396 to Alanine

    Promoter

    CMV

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-flex-oChIEF-citrine

    Plasmid

    #50973

    Purpose

    flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    inverted and inserted between two lox sites

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PROM5_MpEF1a

    Plasmid

    #136113

    Purpose

    Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM5_MpEF1a Mapoly0024s0116
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pLenti-CaMKIIa-C1C2-EYFP

    Plasmid

    #35519

    Depositor

    Karl Deisseroth

    Article

    Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

    Insert

    ChR1-ChR2 Chimera
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    June 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NylB'_pET21b

    Plasmid

    #215416

    Purpose

    Expression construct for NylB' gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. KBB418

    Plasmid

    #185088

    Purpose

    Swi6L-GFP with nuclear export signal mutated to alanines

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Swi6 nuclear export signal L to A mutations

    Promoter

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mOtop3_pGEMHE

    Plasmid

    #114676

    Purpose

    This construct was used to express mouse Otop3 in Xenopus laevis oocytes

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 3 (Otop3 Mouse)
    

    Use

    For generating the mrna, which can highly express…

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRII-1.1-1.10a

    Plasmid

    #69589

    Purpose

    OT-II TCR a chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR alpha clone 1.1 (Tcra Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pJH001

    Plasmid

    #67641

    Purpose

    High copy Cas9 expression vector with LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    Empty
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ZJOM153

    Plasmid

    #133642

    Purpose

    Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    Vph1-2GFP (VPH1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUDP293

    Plasmid

    #204228

    Purpose

    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

    Depositor

    Jean-Marc Daran

    Article

    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

    Inserts

    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    Saccharomyces cerevisiae PGK1 and Saccharomyces c…

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ZJOM31

    Plasmid

    #133645

    Purpose

    Integration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-TGL3-GFP (TGL3 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFA6a-GST-His3MX6

    Plasmid

    #41604

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. B52_puro_empty_gRNA

    Plasmid

    #197557

    Purpose

    sites for cloning two gRNA with puromycin cassette

    Depositor

    Darius Bägli

    Type

    Empty backbone

    Use

    Empty grna backbone with puromycin resistance gene

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for gRNA, CMV for puromycin resistance.

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBPhsFlp2::PEST

    Plasmid

    #63171

    Purpose

    Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    hsFlp2::PEST
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MXS_CMV::PuroR-bGHpA

    Plasmid

    #62439

    Purpose

    MXS_chaining vector with CMV::PuroR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Puromycin with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRG646_lexO-HO_LexA-TF_LEU2MX

    Plasmid

    #154815

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Codon Optimized Dysferlin

    Plasmid

    #67878

    Purpose

    The human dysferlin cDNA was codon optimized in an attempt to increase Dysferlin production in human cells.

    Depositor

    Matthew Hirsch

    Article

    Hirsch et al Mol Ther. 2013 Dec;21(12):2205-16. doi:

    Insert

    codon optimized Dysferlin
    

    Use

    Unspecified

    Tags

    No

    Expression

    Mutation

    Promoter

    No Promoter

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pYZ173

    Plasmid

    #98410

    Purpose

    Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. ptrc99a-pVhb-tdTER-fdh

    Plasmid

    #215649

    Purpose

    pBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Inserts

    trans-enoyl-CoA reductase
    formate dehydrogenase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. gRNA_tdtomato_reporter1_SP-2x

    Plasmid

    #79370

    Purpose

    Assays activity of transcriptional activators fused to SP Cas9

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    tdtomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX-2T-RNAPII-CTD wt #14-HA

    Plasmid

    #160688

    Purpose

    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

    Depositor

    Jeffry Corden

    Article

    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

    Insert

    RNA Polymerase II CTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C terminal domain only

    Promoter

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pXZX501

    Plasmid

    #169455

    Purpose

    pRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassette

    Depositor

    Jef Boeke

    Article

    Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

    Insert

    gRNA for MATa
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. mOtop2_pGEMHE

    Plasmid

    #114675

    Purpose

    This construct was used to express mouse Otop2 in Xenopus laevis oocytes

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 2 (Otop2 Mouse)
    

    Use

    For generating the mrna, which can highly express…

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pXZX351

    Plasmid

    #169454

    Purpose

    pRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassette

    Depositor

    Jef Boeke

    Article

    Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

    Insert

    gRNA for MATα
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGL3-VEGFR2-225

    Plasmid

    #21306

    Depositor

    Donald Ingber

    Article

    Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

    Insert

    VEGFR2 promoter (KDR Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

44. pET-NtLNtC60αC20C60β

    Plasmid

    #160874

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcL: pBAD, chaperones: T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pPMVAu5 (R)

    Plasmid

    #98296

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. mOtop2_pcDNA3

    Plasmid

    #114678

    Purpose

    This construct was used to express mouse Otop2 in HEK-293 cells

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 2 (Otop2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLenti-CaMKIIa-C1C2-TS-EYFP

    Plasmid

    #35520

    Depositor

    Karl Deisseroth

    Article

    Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

    Insert

    ChR1-ChR2 Chimera
    

    Use

    Lentiviral

    Tags

    Trafficking signal and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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48. NavBp

    Plasmid

    #100014

    Purpose

    Electrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFP

    Depositor

    David Clapham, Paul DeCaen

    Article

    DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

    Insert

    NavBp
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pRK010

    Plasmid

    #69874

    Purpose

    expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

    Depositor

    Robert Kittel, Georg Nagel

    Article

    Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

    Insert

    Channelrhodopsin-2
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    D156C (XXL)

    Promoter

    Drosophila Hsp70

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pYZ146

    Plasmid

    #98406

    Purpose

    Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.leu1 (leu1 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge