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Showing: 1 - 50 of 12522 results

  1. pET28a-LIC

    Plasmid
    #26094
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26094-plasmid-map-sequence-id-246717

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  2. pET21a-LIC

    Plasmid
    #62303
    Purpose
    E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62303-plasmid-map-sequence-id-102191

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  3. pCW-LIC

    Plasmid
    #26098
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Three consecutive Taq promoters, 2 active
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26098-plasmid-map-sequence-id-251071

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  4. pTRC-LIC

    Plasmid
    #62343
    Purpose
    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter from pKK233-2
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62343-plasmid-map-sequence-id-102266

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  5. p28BIOHTEV-LIC

    Plasmid
    #167852
    Purpose
    Bacterial expression vector with N-terminal AviTag and C-terminal 6X-his tag for protein expression and purification
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 22, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167852-plasmid-map-sequence-id-331065

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  6. pET28GST-LIC

    Plasmid
    #26101
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6XHis, GST, and Thrombin CLeavage Site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26101-plasmid-map-sequence-id-142370

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  7. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pET32a-LIC

    Plasmid
    #62310
    Purpose
    E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis, S-tag, and Trx-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62310-plasmid-map-sequence-id-326570

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  9. p28BIOH-LIC

    Plasmid
    #62352
    Purpose
    E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62352-plasmid-map-sequence-id-102272

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  10. pFBOH-LIC

    Plasmid
    #26099
    Purpose
    SGC Empty baculovirus expression/transfer vector
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    6x His and Thrombin protease cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26099-plasmid-map-sequence-id-12255

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  11. LIC-Z

    Plasmid
    #153543
    Purpose
    perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication
    Depositor
    Katharina Gaus
    Article
    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    NA
    Promoter
    CMV
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    153543-plasmid-map-sequence-id-305901

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  12. pET28GSTlinker-LIC

    Plasmid
    #119970
    Purpose
    Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119970-plasmid-map-sequence-id-232695

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  18. IDG_CLIC3_OE_1

    Plasmid
    #161657
    Purpose
    Overexpresses CLIC3
    Depositor
    Mike McManus
    Insert
    CLIC3 (CLIC3 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    161657-plasmid-map-sequence-id-319638

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  20. IDG_CLIC5_OE_1

    Plasmid
    #161641
    Purpose
    Overexpresses CLIC5
    Depositor
    Mike McManus
    Insert
    CLIC5 (CLIC5 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    161641-plasmid-map-sequence-id-322807

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  21. pSLICER

    Plasmid
    #197288
    Purpose
    Conjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transfer
    Depositor
    Bogumil Karas
    Article
    Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.
    Insert
    I-SceI Endonuclease
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197288-plasmid-map-sequence-id-393036

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  27. IDG_CLIC2_OE_1

    Plasmid
    #161642
    Purpose
    Overexpresses CLIC2
    Depositor
    Mike McManus
    Insert
    CLIC2 (CLIC2 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    161642-plasmid-map-sequence-id-319579

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  34. LIC-Z-Y-L

    Plasmid
    #153546
    Purpose
    this is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucine
    Depositor
    Katharina Gaus
    Article
    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    with 6 Y in 3 ITAMs mutated to L
    Promoter
    CMV
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    153546-plasmid-map-sequence-id-305909

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  35. SPLICSFAST-ER-PO-P2A

    Plasmid
    #214894
    Purpose
    Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-ER-PO-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214894-plasmid-map-sequence-id-423480

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  36. DiLiCre 2.0

    Plasmid
    #198752
    Purpose
    A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    Cre-PhoCI-ERT2-ERT2
    Use
    Lentiviral
    Tags
    Cre, kept in a photocleavable site, followed by 2…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TRE3GV
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198752-plasmid-map-sequence-id-397647

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  42. SPLICSFAST-LY-MT-P2A

    Plasmid
    #214897
    Purpose
    Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LY-MT-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214897-plasmid-map-sequence-id-423483

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  43. SPLICSFAST-PO-MT-P2A

    Plasmid
    #214899
    Purpose
    Expresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-PO-MT-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214899-plasmid-map-sequence-id-423486

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  44. SPLICSFAST-NU-MT-P2A

    Plasmid
    #214898
    Purpose
    Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-NU-MT-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214898-plasmid-map-sequence-id-423484

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  45. ER-SPLICSs-cFAST10

    Plasmid
    #210431
    Purpose
    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    ER-SPLICSs-cFAST10
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210431-plasmid-map-sequence-id-415626

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  46. SPLICSFAST-ER-MT-P2A

    Plasmid
    #214892
    Purpose
    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-ER-MT-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214892-plasmid-map-sequence-id-423478

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  47. pETcLIC-GFP

    Plasmid
    #183514
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183514-plasmid-map-sequence-id-362488

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  49. pFHMSP-LIC-C

    Plasmid
    #26100
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    8x His tag and MKFLVNVALVFMVVYISYIYAAA (Honeybee …
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  50. pFHMSP-LIC-N

    Plasmid
    #26095
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Sept. 15, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1 - 50 of 12522 results