375 results

1. Human SAM Library in lenti sgRNA(MS2)_zeo backbone

   Pooled Library

   #1000000057

   Purpose

   Genome-wide CRISPR gRNA pooled library for activation of human genes

   Depositor

   Feng Zhang

   Available since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pMuSIC pool v1.0

   Pooled Library

   #227193

   Purpose

   Two-way barcodes from 18 fluorescent proteins for mammalian expression. Can be used for clone tracing, or for pairing with perturbations for single cell resolution in spectral detection measurements.

   Depositor

   Marc Birtwistle

   Article

   Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

   Expression

   Mammalian

   Available since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

3. RLC11

   Pooled Library

   #163955

   Purpose

   RLC11 pooled library

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Expression

   Bacterial

   Use

   CRISPR

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

4. mCRISPRi-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

   Pooled Library

   #83994

   Purpose

   Mouse CRISPRi Pooled Library targeting membrane proteins

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

5. RLC12

   Pooled Library

   #163954

   Purpose

   RLC12 pooled library

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Expression

   Bacterial

   Use

   CRISPR

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

6. Mouse Metabolic Gene CRISPRa sgRNA library

   Pooled Library

   #187081

   Purpose

   Lentiviral CRISPRa library targeting mouse metabolic genes for activation of gene expression

   Depositor

   Kivanc Birsoy

   Article

   Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

   Expression

   Mammalian

   Species

   Mus musculus

   Use

   Lentiviral

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

7. mCRISPRi-v2, Stress and Proteostasis (m3), top 5 sgRNAs/gene

   Pooled Library

   #83991

   Purpose

   Mouse CRISPRi Pooled Library targeting stress and proteostasis genes

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

8. Humagne Set D

   Pooled Library

   #172651

   Purpose

   Set D contains best-ranked guides 2 and 3.

   Depositor

   John Doench, David Root

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Expression

   Mammalian

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

9. Xiaole Shirley Liu lab Human CRISPR lentiviral library

   Pooled Library

   #1000000132

   Purpose

   CRISPR gRNA knockout pooled library for targeting the human genome

   Depositor

   Xiaole Shirley Liu

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

10. hCRISPRi_dual_5_6

    Pooled Library

    #187248

    Purpose

    Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Species

    Synthetic

    Use

    Lentiviral

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

11. Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned1

    Pooled Library

    #101932

    Purpose

    Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned1. Coexpresses mCherry.

    Depositor

    Michael Bassik

    Article

    Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

    Expression

    Mammalian

    Use

    CRISPR

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

12. Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050, Set A

    Pooled Library

    #104090

    Purpose

    Crispri

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Use

    Lentiviral

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

13. PCT48

    Pooled Library

    #170392

    Purpose

    Barcoded plasmid library

    Depositor

    Jonathan Weissman

    Article

    Jones et al Genome Biol. 2020 Apr 14;21(1):92. doi:

    Expression

    Mammalian

    Species

    Synthetic

    Use

    CRISPR and Lentiviral

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

14. hCRISPRa_dual_1_2

    Pooled Library

    #187249

    Purpose

    Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Species

    Synthetic

    Use

    Lentiviral

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

15. LX-miR

    Pooled Library

    #112200

    Purpose

    gRNA library targets human primary miRNAs

    Depositor

    Ren-Jang Lin

    Article

    Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

16. Mouse sgRNA library Gouda in pRDA_118 backbone

    Pooled Library

    #136987

    Purpose

    Expresses sgRNAs targeting mouse genes

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

17. hCRISPRi-v2, Mitochondria, Trafficking, and Motility (h4), top 5 sgRNAs/gene

    Pooled Library

    #83974

    Purpose

    Human CRISPRi Pooled Library targeting Mitochondria, Trafficking, and Motility genes

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

18. hCRISPRi-v2, Gene Expression (h5), top 5 sgRNAs/gene

    Pooled Library

    #83975

    Purpose

    Human CRISPRi Pooled Library targeting gene expression

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

19. Barcode Library 0 (BCL0)

    Pooled Library

    #179851

    Purpose

    Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code ACGT.

    Depositor

    Jason Moffat

    Article

    Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

    Expression

    Mammalian

    Use

    Lentiviral

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

20. E.coli_GW-sgRNA_Lib3

    Pooled Library

    #113136

    Purpose

    Genome-wide gRNA library for E. coli, sub3

    Depositor

    Chong Zhang

    Article

    Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

    Expression

    Bacterial

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

21. E.coli_GW-sgRNA_Lib1

    Pooled Library

    #113134

    Purpose

    Genome-wide gRNA library for E. coli, sub1

    Depositor

    Chong Zhang

    Article

    Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

    Expression

    Bacterial

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

22. mCRISPRi-v2, Gene Expression (m5), top 5 sgRNAs/gene

    Pooled Library

    #83993

    Purpose

    Mouse CRISPRi Pooled Library targeting gene expression

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

23. E.coli_GW-sgRNA_Lib4

    Pooled Library

    #113137

    Purpose

    Genome-wide gRNA library for E. coli, sub4

    Depositor

    Chong Zhang

    Article

    Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

    Expression

    Bacterial

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

24. E.coli_GW-sgRNA_Lib2

    Pooled Library

    #113135

    Purpose

    Genome-wide gRNA library for E. coli, sub2

    Depositor

    Chong Zhang

    Article

    Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

    Expression

    Bacterial

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

25. E.coli_NC_sgRNA_lib

    Pooled Library

    #113146

    Purpose

    Genome-wide gRNA library for E. coli, non-targeting control

    Depositor

    Chong Zhang

    Article

    Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

    Expression

    Bacterial

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

26. TRE-MPRA_minPromega Library

    Pooled Library

    #201013

    Purpose

    Massively parallel reporter assay (MPRA) library with minPromega promoter

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Expression

    Mammalian

    Use

    Luciferase

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

27. SPLINTR BFP v1

    Pooled Library

    #179776

    Purpose

    SPLINTR (Single-cell Profiling and LINeage TRacing) is a high diversity lentiviral barcode library for tracing clonal lineages using bulk and single-cell readouts.

    Depositor

    Mark Dawson

    Article

    Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

    Expression

    Mammalian

    Use

    Lentiviral

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

28. mCRISPRi-v2, Cancer and Apoptosis (m2), top 5 sgRNAs/gene

    Pooled Library

    #83990

    Purpose

    Mouse CRISPRi Pooled Library targeting cancer and apoptosis genes

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

29. mCRISPRi-v2, Mitochondria, Trafficking, and Motility (m4), top 5 sgRNAs/gene

    Pooled Library

    #83992

    Purpose

    Mouse CRISPRi Pooled Library targeting Mitochondria, Trafficking, and Motility genes

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

30. mCRISPRi-v2, Unassigned (m7), top 5 sgRNAs/gene

    Pooled Library

    #83995

    Purpose

    Mouse CRISPRi Pooled Library targeting unassigned genes

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

31. Human CRISPR lncRNA activation library (SAM) in lenti sgRNA(MS2)_zeo backbone

    Pooled Library

    #1000000106

    Purpose

    Human CRISPR Synergistic Activation Mediator (SAM) pooled library for the transcriptional activation of lncRNAs

    Depositor

    Feng Zhang

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

32. Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned3

    Pooled Library

    #101934

    Purpose

    Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned3. Coexpresses mCherry.

    Depositor

    Michael Bassik

    Article

    Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

    Expression

    Mammalian

    Use

    CRISPR

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

33. Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned2

    Pooled Library

    #101933

    Purpose

    Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned2. Coexpresses mCherry.

    Depositor

    Michael Bassik

    Article

    Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

    Expression

    Mammalian

    Use

    CRISPR

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

34. pre-associated oBC-minP-GFP-mBC backbone (p043)

    Pooled Library

    #194098

    Purpose

    pre-associated oBC-minP-GFP-mBC backbone (p043), can directly be used to deposit library of regulatory elements

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

35. Human SLC SAM Library

    Pooled Library

    #132561

    Purpose

    Expresses gRNAs for activation of human Solute Carrier family genes

    Depositor

    Giulio Superti-Furga

    Article

    Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

36. Liu Lab MusCK Library A

    Pooled Library

    #174196

    Purpose

    MusCK library contains 23,622 unique sgRNAs targeting 4,787 gene locations in the genome. Sub-library MusCK A contains 5 gRNAs per gene.

    Depositor

    Xiaole Shirley Liu

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5357-5374.e22.

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

37. Splicing-targeting CRISPR-Cas9 library for human lncRNAs

    Pooled Library

    #119977

    Purpose

    The CRISPR sgRNA library was designed to target the 5' or 3' splice sites of lncRNAs in genome-scale, in order to knockout the targeted lncRNAs.

    Depositor

    Wensheng Wei

    Article

    Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

38. Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1

    Pooled Library

    #1000000100

    Purpose

    CRISPR gRNA knockout pooled library (version 1) for targeting the human genome

    Depositor

    David Sabatini, Eric Lander

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

39. E.coli_GW-sgRNA_Lib5

    Pooled Library

    #113138

    Purpose

    Genome-wide gRNA library for E. coli, sub5

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Expression

    Bacterial

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

40. Mouse Liver CRISPR Knockout Library

    Pooled Library

    #192824

    Purpose

    CRISPR screening library containing ~72,000 sgRNAs

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

41. Human messenger-RBP sgRNA library

    Pooled Library

    #168791

    Purpose

    CRISPR library targets sub-pool of human mRNA binding proteins

    Depositor

    Martin Turner

    Article

    Turner et al CRISPR J. 2021 Jun;4(3):427-437. doi: 10

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

42. mCRISPRa-v2, Kinases, Phosphatases, and Drug Targets (m1), top 5 sgRNAs/gene

    Pooled Library

    #83998

    Purpose

    Mouse CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug Targets

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

43. p11-3’_8xN_PAM-site1 (RTW554)

    Pooled Library

    #160132

    Purpose

    Plasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Species

    Synthetic

    Use

    CRISPR

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

44. TP53 MITE Library

    Pooled Library

    #113569

    Purpose

    Pooled lentiviral plasmid library containing ~8,000 single amino acid substitution and nonsense mutant variants of p53

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

45. TBG-mTSG

    Pooled Library

    #113585

    Purpose

    AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes in mouse liver

    Depositor

    Sidi Chen

    Article

    Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

    Expression

    Mammalian

    Use

    AAV, CRISPR, Lentiviral, and Luciferase

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

46. LV2btCRISPRko.v1

    Pooled Library

    #213927

    Purpose

    Uses lentivirus to deliver the library into hard-to-transduce cells

    Depositor

    Wenfang Spring Tan

    Article

    Tan et al PLoS Pathog. 2023 Dec 6;19(12):e1011822.

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

47. Liu Lab MusCK (A + B)

    Pooled Library

    #1000000178

    Depositor

    Xiaole Shirley Liu

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

48. LARRYv2-T-Sapphire library 2

    Pooled Library

    #233214

    Purpose

    Barcoding pooled library

    Depositor

    Alejo Rodriguez-Fraticelli

    Article

    Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

    Species

    Synthetic

    Use

    Lentiviral

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

49. scQers promoter control #5 (high: EEF1A1 promoter, p028)

    Pooled Library

    #217424

    Purpose

    scQers promoter control #5 (high: EEF1A1 promoter, p028)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

50. mCRISPRa-v2, Cancer and Apoptosis (m2), top 5 sgRNAs/gene

    Pooled Library

    #83999

    Purpose

    Mouse CRISPRa Pooled Library targeting cancer and apoptosis genes

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only