...29;7:12266. David Savage Maltose Monitor maltose levels and metabolism (FLIPmal) Fluorescence Resonance...:19562. Takashi Tsuboi Maltose Single fluorescent protein biosensor for maltose Rapid construction of ...Jun 3. 1(1):11. Wolf Frommer Maltose Green fluorescent MBP-based maltose biosensor A genetically encoded...Biosensors Fluorescent Proteins: Biosensors Metal Ions Small Molecules Neurotransmitters Cell Physiology Browse...Diverse Functional Payloads to Cells of Behaving Mammals. Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-...tethering by PDZD8 regulates Ca(2+) dynamics in mammalian neurons. Science. 2017 Nov 3;358(6363):623-630...

...available for expression in mammalian systems, bacteria, plants, and Drosophila. Mammalian ID Plasmid Gene/Insert...GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor Mammalian hU6 epegRNA...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... activity; can be used in PE2–PE5 systems PE6 - small prime editors with optimized RT and/or Cas9 domains...Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6 pegRNA BsaI No mRFP1 David Liu 140448 QPM-... No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI for sgRNAs; BbsI...sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob ...

...3xFNLDD in mammalian cells 48880 3xFNLDD/pMXs-I2 iChIP plasmid, expresses 3xFNLDD in mammalian cells 48972...3xFNLDD in mammalian cells 49536 3xFNLDD/pMXs-puro iChIP plasmid, expresses 3xFNLDD in mammalian cells 51240...7.1 enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...3xFLAG-dCas9/pMXs-puro Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...3xFLAG-dCas9/pMXs-IG Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...3xFLAG-dCas9/pMXs-I2 Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...3xFLAG-dCas9/pMXs-neo Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...

...Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a mammalian cell line...Although we will use the example of CRISPR/Cas9 in mammalian cells, many of these principles apply to using...dCas9-KRAB is more effective than dCas9 alone for mammalian cell lines. Activate (CRISPRa) Increase expression...expression systems and variables for using CRISPR in mammalian cells. Some of the variables include: Species ...Expression System Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive...expression of Cas9 and/or gRNA in a wide variety of mammalian cell lines Useful for difficult to transfect cell... ∼4.5 kb packaging limit (only compatible with smaller Cas enzymes) Transient or stable expression of ...

...-Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled...destination vectors and a reporter vector for mammalian cell-based validation assay that are designed ...vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing unique... contains the VP64 domain (four repeats of the minimal activation domain of VP16) at the C terminus. 47389...Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize...plasmids were optimized for TALEN expression in mammalian systems including hard-to-transfect cell lines...sequence to drive efficient TALEN expression in mammalian cells, (iv) an improved, truncated TALE backbone...

...Bacterial systems were tested for functionality in mammalian cells. The lac system with the inducer isopropylthio-β-galactoside... Gossen and Bujard tested the tet system in a mammalian cell system (HeLa) and found that the tet system...promoters are developed by placing a TRE upstream of a minimal promoter. Tetracycline Off System The initial system...promoter (Pbi); Pbi contains a TRE between two minimal CMV and is silent in absence of binding of tTA ...knockdown of gene expression at a single copy in mammalian cells; Expresses firefly luciferase hairpin and...References Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Gossen...

...affecting females at an incidence of approximately 1:10,000. It is characterized by apparently normal development...Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources...criteria, defined as the presence of relatively normal early development, regression with a loss of spoken... rare cases Rett syndrome can be inherited. In females, random X inactivation results in approximately...PMID: 21074045 2017 - the Bird lab determines a minimally functional MECP2 that rescues neurological defects...opens in a new window) PMID: 29019980 Return to top Animal Models Studies of the MECP2 protein in mouse and...general introduction to CRISPR technology or the mammalian CRISPR resources for a full selection of plasmids...

... nuclease domains: pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in... domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be used as a substitute...domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802 (for expression of...domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette...domain ( Sollu et al., Nucleic Acids Res ) in mammalian cells or zebrafish). pCLR2068 (for expression ...domain. ( Doyon et al., Nat Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al...

...2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell culture. Five ... specialized Cas proteins snip foreign DNA into small fragments approximately 20 bp in length and paste...characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9... improving targeting in AT-rich genomes. Cpf1's small size also makes it suitable for multiplexing and...permanent genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require a specific... this non-specific cleavage does not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also ...has CRISPR plasmids for the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. ...

...are sporadic, having no prior family history. A small percentage (5-10%) are familial ALS cases having...These familial cases, usually inherited in an autosomal dominant manner, are associated with known genetic... Disease Huntington’s disease (HD) is a fatal autosomal dominant genetic disorder that causes the progressive...difficulty speaking and swallowing. HD is caused by abnormal expansion of a repeating CAG triplet series within...mutated HTT gene will have huntingtin proteins with abnormally long polyglutamine sequences. These glutamine-rich...medication and surgery. PD primarily involves the malfunction and death of dopamine-producing neurons in the... specific known cause) nature of the disease, a small portion of cases can be attributed to known genetic...

...(SARS classic) sequences. Mammalian Targets : Plasmids containing mammalian sequences linked to SARS-CoV...Return to top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as...protein constructs for making VSV pseudo virus Mammalian constructs Due to the size and complexity of the...entry into cells. ( 4 ) Cathepsin L - a cysteine endosomal protease that triggers proteolysis of SARS-CoV...plasmids from Andrew Ellington's lab. High-surety isothermal amplification and detection of SARS-CoV-2, including...Andersen, KG, et al, 2020, Nature Medicine The proximal origin of SARS-CoV-2 . TMPRSS2 - Hoffmann M, et...Andersen, KG, et al, 2020, Nature Medicine The proximal origin of SARS-CoV-2 . BSG - Wang, K et al, 2020...

...sources of antibodies for research purposes include animals or hybridoma cells, but are difficult to generate...into plasmids and expressing these in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits...recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are derived from ...the plasmids are transiently transfected into mammalian tissue culture cells, the R-mAbs are secreted ...use. Other scientists may choose to work with a smaller fragment of an antibody, which could offer increased...increased stability and ease of production due to their small size. These recombinant antibody fragments can be...antibody heavy- and light-chain DNAs into a single mammalian expression vector A tool kit for rapid cloning...

...DHLAG, HLADG, Ia-GAMMA CD8A CD8a molecule CD8, Leu2, MAL, p32 CD8B CD8b molecule CD8B1, LYT3, Leu2, Ly3, MGC119115...cytotoxicity triggering receptor 3 1C7, CD337, LY117, MALS, NKp30 NFAT5 nuclear factor of activated T-cells...TRAP, gp39, hCD40L CD8A CD8a molecule CD8, Leu2, MAL, p32 CD8B CD8b molecule CD8B1, LYT3, Leu2, Ly3, MGC119115... HSCR, HSCR2 EGF epidermal growth factor (beta-urogastrone) HOMG4, URG EGFR epidermal growth factor receptor...Categories Plasmid Tables Additional Resources Mammalian organisms are exposed to millions of potential...Chemokines Chemokines, or chemotactic cytokines, are small proteins that can be secreted by immune cells and...X-associated protein - SHFM1 split hand/foot malformation (ectrodactyly) type 1 DSS1, ECD, SEM1, SHFD1...

...RPS6KA2 RPS6KA3 RPS6KA6 Ribosomal protein S6 kinase RPS6KB RPS6KB1 RPS6KB2 Ribosomal protein S6 kinase B1...collection of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active Ras is the most...Plasmids Ras Gene List Resources Background Ras is a small GTPase and is a member of the G protein (guanine...addition and removal of a phosphate group. Under normal physiological conditions GTPases play prominent...factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor EIF4EBP EIF4EBP1 EIF4EBP2...Ras-related C3 botulinum toxin substrate (rho family, small GTP binding protein Rac) RAF AFAF BRAF RAF1 Serine...

...localization. ACP and MCP tags ACP- and MCP-tags are small protein tags based on the acyl carrier protein. ...SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag...CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B Control Plasmid CLIP-tag...Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag...MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI Control Plasmid ACP... Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic role of the ...

... that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems, Cas13a does...available for expression in mammalian systems, bacteria, and plants. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

...for expression in mammalian systems, bacteria, plants, yeast, and zebrafish. Mammalian ID Plasmid Gene/...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...and they can convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently...editors are designed to work in a very narrow window proximal to the PAM sequence, some base editing systems...

...GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal Brain vascular cells...GRE or transgenic animal L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4 - AAV.CAP-B10...with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For PNS applications ...

...fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector Hillel Adesnik 107708...channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by...export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome...genetically encoded dopamine sensor RdLight1 in mammalian cells. Lin Tian 125708-AAV9 pAAV-hSynapsin1-RdLight1...genetically encoded dopamine sensor RdLight1 in mammalian neurons. Lin Tian 127090-PHPeB pAAV-CAG-DIO-ChR2... in AAV production vector expressed under the mammalian promoter (EF1a) Francois St-Pierre 179460-AAV1...AAV production vector under the control of the mammalian promoter (EF1a) Francois St-Pierre 179463-AAV9...

...into cells. Protocol for Genomic Deletions in Mammalian Cell Lines CasPEDIA : An encyclopedia of Class...tool for CRISPR/Cas design Program for designing optimal gRNAs. Provides feedback on number of potential...PAMs. Currently supports: mouse and human. CRISPR Optimal Target Finder Tool from the O'Connor-Giles Lab ...cloning pCRISPR PDF 106.7 KB Mendenhall & Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor...Orkin and Bauer Protocol for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) Link Parrott NEBuilder...

...for CRISPR plasmids for RNA editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI Publication...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...truncation retain RNA editing capabilities but are small enough to be packaged in AAV particles. Want more...

...are available for expression in mammalian systems and bacteria. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... used in bacteria genome editing, though some mammalian systems have been developed. Figure 1: Overview...

...nuclease system to facilitate genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ....Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9... are two sets of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene ...adeno-associated virus (AAV). The single vector system uses a smaller Cas9 from S. aureus (SaCas9) that has been human...In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich...

...CAGAACACCCCCATCGGCGA 72619 cut S. pyogenes 26493208 Guigo, Johnson Malat1 H. sapiens gRNA1: GAACCGGTGGGGCTGCGTCA; gRNA2: ...GGCAGGAGAGGCCAGTTGCG 72620 cut S. pyogenes 26493208 Guigo, Johnson Malat1 H. sapiens gRNA1: GCAACTTCCATTTTCAGTCT; gRNA2: ...GGAAGCCTCAGCTCGCCTGA 72621 cut S. pyogenes 26493208 Guigo, Johnson Malat1 H. sapiens gRNA1:GCTGGGGCTCAGTTGCGTAA; gRNA2:AGGTTTCTAAAACATGACGG...AGGTTTCTAAAACATGACGG 72622 cut S. pyogenes 26493208 Guigo, Johnson Malat1 H. sapiens gRNA1:GTTGAGATGAAGCTTCTTCA; gRNA2:TCAACCGTCCCTGCAAGGCT...TCAACCGTCCCTGCAAGGCT 72623 cut S. pyogenes 26493208 Guigo, Johnson Malat1 H. sapiens gRNA1: GAAACCTCGTGTAGCTATCA; gRNA2: ...

...bind to a small molecule, the reporter plasmid can be used to detect the presence of that small molecule... have been well studied or can be controlled by small molecules, light, temperature, or even intracellular...plasmid. Other similar reporters for detecting maltose can be found here . pAra Series Various Plasmids...

...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...Human Bradley 3rd ~9 pairs/exon 9,508 Mouse CRISPR Proximal Poly(A) Site Knockout Library 190744 Knockout ... 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout Human Sabatini...Pan-Druggable Cancer Library 182133 Knockout Human Mali 3rd 1 74 Perturb-seq Guide Barcodes (GBC) 85968 ...

... available for expression in mammalian systems and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

...tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell...Fibroblasts Cholinergic Neurons Lentiviral Human Small molecules enable neurogenin 2 to efficiently convert... Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts Neurons Lentiviral Human REST suppression...

... for epigenetic modification in mammalian or plant systems. Mammalian ID Plasmid Gene/Insert Promoter ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

...available for expression in mammalian systems, bacteria, plants, and yeast. Mammalian ID Plasmid Gene/Insert...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...