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We narrowed to 1,052 results for: FER

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Showing: 101 - 150 of 1052 results

  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...function of the Cas protein (cut, nick, activate, interfere, etc.). gRNA-only plasmids are not limited to ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Lentiviral Preps CRISPR Protocols...

  2. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  3. Plasmid Collections

    Type
    Collection
    ...Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus...plasmids all built with the same backbone and only differing in a small region. Click here to view all pooled...

  4. Serotype Testing AAV

    Type
    Collection
    ...reporters and can be used to compare the tropism of different serotypes. In addition to the standard 100 µL ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  5. Adenovirus Plasmids

    Type
    Collection
    ...need to use two plasmids: A shuttle plasmid (or transfer plasmid) containing the transgene of interest,...contains β-gal and GFP and a ∼10 Kb genomic DNA stuffer Vogelstein 18703 pEZYshuttle Modified pShuttle-...

  6. Recombinases AAV Preps

    Type
    Collection
    ... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  7. AAV for Neuronal Tracing

    Type
    Collection
    ...Resources Addgene Blog: Rabies and Neuronal Tracing References Wickersham IR, Finke S, Conzelmann KK, Callaway...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  8. Antibody Plasmid Collection

    Type
    Collection
    ... a smaller fragment of an antibody, which could offer increased stability and ease of production due to...antibodies. BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system to rapidly engineer the constant...

  9. Chemogenetics AAV Preps

    Type
    Collection
    ... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  10. Genetic Code Expansion

    Type
    Collection
    ... directed evolution to charge the tRNA with a different amino acid. To achieve these modifications, two...Farren Isaacs Return to top Other Resources and References GCE4All Research Center GCE4All: Making Genetic...Phosphorylation Rinehart Lab Phosphorilation Reagents References Chin, J. (2017). Expanding and reprogramming ...

  11. Microbiology Resources

    Type
    Collection
    ...by Microbe System Plasmids for Archaea Species Haloferax sp. Methanococcus maripaludis Methanothermobacter...introduction to CRISPR technology. Check out our CRISPR reference page for CRISPR resources, such as depositor protocols...

  12. TALEN Guide

    Type
    Collection
    ...of 33-35 amino acid repeats. These repeats only differ from each other by two amino acids, their repeat-variable... Addgene’s TAL effector and TALEN kits here . References Efficient design and assembly of custom TALEN...

  13. Viral Production

    Type
    Collection
    ...Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding Rep and serotype-specific... taken off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching...

  14. Caltech Systemic Capsids

    Type
    Collection
    ...performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  15. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... is especially amenable to genome engineering, offering flexibility and easy multiplexing/scaling. Prokaryotes.... cerevisiae and S. pombe ) Zebrafish Xenopus References Barrangou R, Fremaux C, Deveau H, Richards M,...

  16. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...interest with a fluorescent protein that has a different emission wavelength. For example, if the marker... Return to top Looking for more constructs or different fluorescent protein markers? Check out the Michael...

  17. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  18. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  19. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  20. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  21. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

  23. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  26. CRISPR Plasmids - Parasites

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  28. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  29. Fluorescent Protein Guide

    Type
    Collection
    ...receptors that affect cell signalling pathways. Luciferase Plasmids Use bioluminescent reporters for studying...

  31. AAV Viral Preps

    Type
    Collection
    ... Packaged on Request service. You can choose a transfer plasmid from our repository and request packaging...

  34. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  36. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...photoreceptor and bipolar cell fate specification, yet differs from deleting both genes . Development. doi: 10.1242...

  39. CRISPR Plasmids and Resources

    Type
    Collection
    ... activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor peptide, ...

  40. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  41. Synthetic Biology - Overview

    Type
    Collection
    ...Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard...

  43. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  44. AAV Molecular Tools

    Type
    Collection
    ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  45. Brain Armamentarium

    Type
    Collection
    ...molecular genetic reagents to gain access to many different brain cell types. The expanded collection of Armamentarium...

  46. The Pleiades Promoter Project

    Type
    Collection
    ...NLS N/A CAG promoter pEMS1488 intron-lacZ/NLS References Portales-Casamar, E., Swanson, D. J., Liu, L....

  47. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Plasmids scFv Plasmids Nanobody Plasmids Protocols References The UC Davis/NIH NeuroMab Facility (Link opens...NeuroMab Protocols (Link opens in a new window) References Andrews NP, et al. 2019. A toolbox of IgG subclass-switched...

  48. Molecular Biology Reference

    Type
    Guide
    ...Educational Resources Molecular Biology Reference Molecular Biology Reference Jump To: Origins of Molecular Genetics...plasmids contain a reporter gene (e.g., luciferase or GFP) that offers a readout of the activity of the genetic...Next-generation Sequencing Long-read Sequencing Reference Tables Additional Resources Plasmids are one of...same amino acid — a phenomenon called degeneracy. Refer to the amino acid table below for codon-amino acid... Joshua Lederberg coined the term plasmid, in reference to any extrachromosomal heritable determinant....bacterium to another by a type of horizontal gene transfer called conjugation. Like chromosomal DNA, plasmid...information about promoters, check out our promoters reference page . Selectable Marker Used to select for cells...

  49. Lentiviral Vector Guide

    Type
    Guide
    ...packaging genes. However, they are different viruses and require different isoforms of these packaging components...second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — ...vectors separate transfer, envelope, and packaging components of the virus between different plasmids. The...activate the promoter on the transfer plasmid. Many of the lentiviral transfer plasmids that have been deposited...genes. From an experimental standpoint, the main difference between lentiviruses and gamma-retroviruses is...three (or four, for third generation) plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA ...systems, due to a wide range of infectivity for different cell types (known as tropism). Lentiviral vectors...

  50. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...optimizing your transfer buffer.  Adjusting the buffer For wet and semi-dry transfers, you can adjust ...methanol concentration in transfer buffer is < 20% Wet transfer Increase transfer time Increase voltage...decrease alcohol in transfer buffer Small proteins: Over-transferring Transfer with two membranes...increase alcohol in transfer buffer Mixed proteins: Transferring issues Transfer with two membranes...be under-transferring.  Membrane pore size Membranes come in different pore sizes for different proteins...large.  Transfer method   Wet transfers are more efficient, so if you’re struggling to transfer a large...consider using a wet transfer with a long transfer time. Be aware that the transfer works by electricity...
Showing: 101 - 150 of 1052 results