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Showing: 1 - 50 of 359 results

  10. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Jill Wildonger, Kate O'Connor-Giles, Melissa Harrison
    Article
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Expression
    Mutation
    Silent change from C to A at bp 834 of insert
    Promoter
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    45945-plasmid-map-sequence-id-116251

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  11. PRE-Hsp70BbCas9_1.2

    Plasmid
    #190796
    Purpose
    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.
    Depositor
    Omar Akbari
    Article
    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024
    Insert
    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    190796-plasmid-map-sequence-id-376571

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  17. PRE-Hsp70BbCas9_1.3

    Plasmid
    #190797
    Purpose
    aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product
    Depositor
    Omar Akbari
    Article
    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024
    Insert
    2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    190797-plasmid-map-sequence-id-376572

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  18. PP-HSP70 (3L6Q)

    Plasmid
    #25289
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd2_20:S12-N394 (cgd2_20 Cryptosporidium parvum)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S12-N394
    Promoter
    Available since
    Sept. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. PP-HSP70 (3L4I)

    Plasmid
    #25291
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd2_20:G15-E396
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains amino acids G15-E396
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

    Plasmid
    #216421
    Purpose
    Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.
    Depositor
    Ellen Bushell
    Article
    Jonsdottir et al bioRxiv 2024.04.20.590404
    Type
    Empty backbone
    Use
    CRISPR; Expression in plasmodium berghei
    Tags
    3xmyc
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216421-plasmid-map-sequence-id-431291

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  27. hsp70l: hAPOB48-mCherry.

    Plasmid
    #140639
    Purpose
    Heat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.
    Depositor
    Steven Farber
    Article
    Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119
    Inserts
    hsp70l (hsp70l Zebrafish)
    APOB (APOB Human)
    Use
    Zebrafish expression
    Tags
    mCherry
    Expression
    Mutation
    APOB48: amino acid 1-2180
    Promoter
    hsp70l
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140639-plasmid-map-sequence-id-308799

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  47. 2xUAS-1xhsp70-attB

    Plasmid
    #220317
    Purpose
    includes 2 UAS cassettes and 1 heat-shock inducible hsp70 casette
    Depositor
    Erdem Bangi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    220317-plasmid-map-sequence-id-442487

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Showing: 1 - 50 of 359 results